Galr3 (NM_019173) Rat Tagged ORF Clone

CAT#: RR201207

Galr3 (Myc-DDK-tagged ORF) - Rat galanin receptor 3 (Galr3), (10 ug)

Product Images

Circular map for RR201207

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Galr3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR201207 representing NM_019173
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGACATCCAGAACATTTCGCTGGACAGCCCAGGGAGCGTAGGGGCTGTGGCAGTGCCTGTGATCT
  TTGCCCTCATCTTCCTGTTGGGCATGGTGGGCAATGGGCTGGTGTTGGCTGTGCTACTGCAGCCTGGCCC
  AAGTGCCTGGCAGGAGCCAAGCAGTACCACAGATCTCTTCATCCTCAACTTGGCCGTGGCCGACCTTTGC
  TTCATCCTGTGCTGCGTGCCCTTCCAGGCAGCCATCTACACACTGGATGCCTGGCTCTTTGGGGCTTTCG
  TGTGCAAGACGGTACATCTGCTCATCTACCTCACCATGTATGCCAGCAGCTTCACCCTGGCGGCCGTCTC
  CCTGGACAGGTACCTGGCTGTGCGGCACCCACTGCGCTCCAGAGCCCTGCGCACCCCGCGCAACGCGCGC
  GCCGCCGTGGGGCTCGTGTGGCTGCTGGCGGCTCTCTTTTCCGCGCCCTACCTAAGCTATTACGGCACGG
  TGCGCTACGGCGCGCTCGAGCTCTGCGTGCCCGCTTGGGAGGACGCGCGGCGGCGCGCGCTGGACGTGGC
  CACCTTCGCCGCGGGCTACCTGCTGCCGGTGGCCGTGGTGAGCCTGGCCTACGGACGCACGCTATGTTTC
  CTATGGGCCGCCGTGGGTCCCGCGGGCGCGGCGGCAGCAGAGGCGCGCAGACGGGCGACCGGCCGGGCGG
  GACGCGCCATGCTGGCAGTGGCCGCGCTCTACGCGCTTTGCTGGGGCCCGCACCACGCGCTCATCCTCTG
  CTTCTGGTACGGCCGCTTCGCCTTCAGCCCGGCCACCTACGCCTGTCGCCTGGCCTCGCACTGCCTCGCC
  TACGCCAACTCCTGCCTTAACCCGCTCGTCTACTCGCTCGCCTCGCGCCACTTCCGCGCGCGCTTCCGCC
  GCCTGTGGCCCTGCGGCCGTCGCCGCCACCGCCACCACCACCGCGCTCATCGAGCCCTCCGTCGTGTCCA
  GCCGGCGTCTTCGGGCCCCGCCGGTTATCCCGGCGACGCCAGGCCTCGTGGTTGGAGTATGGAGCCCAGA
  GGGGATGCTCTGCGTGGTGGTGGAGAGACTAGACTAACCCTGTCCCCCAGGGGACCTCAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR201207 representing NM_019173
  Red=Cloning site Green=Tags(s)
  
  MADIQNISLDSPGSVGAVAVPVIFALIFLLGMVGNGLVLAVLLQPGPSAWQEPSSTTDLFILNLAVADLC
  FILCCVPFQAAIYTLDAWLFGAFVCKTVHLLIYLTMYASSFTLAAVSLDRYLAVRHPLRSRALRTPRNAR
  AAVGLVWLLAALFSAPYLSYYGTVRYGALELCVPAWEDARRRALDVATFAAGYLLPVAVVSLAYGRTLCF
  LWAAVGPAGAAAAEARRRATGRAGRAMLAVAALYALCWGPHHALILCFWYGRFAFSPATYACRLASHCLA
  YANSCLNPLVYSLASRHFRARFRRLWPCGRRRHRHHHRAHRALRRVQPASSGPAGYPGDARPRGWSMEPR
  GDALRGGGETRLTLSPRGPQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019173
• ORF Size: 1110 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019173.1, NP_062046.1
• RefSeq Size: 1163 bp
• RefSeq ORF: 1113 bp
• Locus ID: 29235
• UniProt ID: O88626
• Cytogenetics: 7q34
• MW: 40.3 kDa
• Gene Summary: binds galanin; mediates activation of GIRK1 and GIRK4 channel inward K+ currents; may play a role in neurotransmitter release [RGD, Feb 2006]