Plin5 (NM_001134637) Rat Tagged ORF Clone

CAT#: RR201130

Plin5 (Myc-DDK-tagged ORF) - Rat similar to lipid droplet associated protein (LOC501283), (10 ug)

Product Images

Circular map for RR201130

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Plin5
• Synonyms: Oxpat
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR201130 representing NM_001134637
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCAGAGAGGCAAAGACACCACCCTAGCTCTACACAGCAGGATGTCCGGTGATCAGACAGCTCAGG
  ACCCTGGATCCAGCCTGGGGGAACTGGACCAGCACAACGTGGTGAAGCGAGTGGTGGCCTTGCCCCTGGT
  CAGGGCCACGTGCACTGCTGTGTCTGGTGCCTACAACTCAGCCAAGGACAGGCACCCGCTGCTGGGCTCT
  GCCTGCCGCTTTGCTGAACACTGTGTGTGTAGTGTGGCTACCTGTGCCCTGGACCACGCACAGCCACTGC
  TGGAGCACCTGCAGCCCAAGTTGGCCACAGTGAATGATCTTGCCTGCAGGGGACTAGACAAATTGGAAGA
  GAAGCTGCCCTTCCTGCAGCAGCCATCGGACACGGTGGTCACCTCAGCCAAGGATGCAGTGGCCAAAAGT
  GTCACAGGCGTGGTGGACCTGGCCCAAAGGGGCCGGCGTTGGAGCGGGGAGCTGAGGCGCTCCGTGAGCC
  AAGCCATGGACACTGTGCTGAGGCGCTCCGTGAGCCAAGCCATGGACACTGTGCTGGGCAAATCAGAGGA
  GCTGGTGGACCATTTCCTGCCCATGACTGAAGCTGAGCTAGTGGCCCTGGCGACTGAGTCCCAGGGCCCA
  GAAGTGGGCTCAGTGGAGGAGCAGAGGCAGAAACAGGGCTACTTTGTGCGTCTGGGATCCCTATCGGCAC
  GCCTCCGCCATCTTGCCTATCAACACTCTTTGGGGAAACTGAGGGAGAGCAAACACCGCACCCAGGAGAT
  GCTGGCCCAGCTGCAGAAAACTCTGGAGCTGATCCAGCATATGCAGAGTAGGGCAAGCCCCACCCCTACT
  TTCCATCACCCAAAGGTCCAGGAGCTTTGCGGGGACTGGAGCCCATGTCTGGAGAATGGCTACCGCCACA
  GCCAGGTGGAGCTGGAGACACTGGCTCTGTCGAGAAGTCTGACCCTGGAGCTGCAGAGTGCAGTGGATGC
  CCTTGCAGGCTGTGTTCGGGGCCTGCCCCCTAGTGCCCAGGCCAAGGTGGCTGAAGTGCAGCGCAGTGTG
  GATGCTCTACAGGCCACCTTTGCTGATGCACACTGCCTTGGTGACGTGGCACCCACTGCTCTGGCTGAGG
  GCCAGGACAGCGTGGCCCAGGCACATGCCTGTGTGGATGAGTTCCTGGATTTGGTCCTTCGAGCCATGCC
  ACTGGCCTGGCTTGTGGGGCCCTTTGCGCCCATCTTGGTGGAGCGGTCAGAGCCCCTGATCAACCTGGCC
  ACCTGTGTGGATGAGGTGGTGGGTGACCCTGACCCCCGCTGGGCACACATGGACTGGCCAGCCCAGCAGA
  GGGCCTGGGAGGCTGAGCCTGCAGACCCTGGGGGACAAGAGGCTGAGCCCCCACTGGGCCAGGTCAAGCA
  CACAATGATGCCAGAGCTAGACTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR201130 representing NM_001134637
  Red=Cloning site Green=Tags(s)
  
  MDQRGKDTTLALHSRMSGDQTAQDPGSSLGELDQHNVVKRVVALPLVRATCTAVSGAYNSAKDRHPLLGS
  ACRFAEHCVCSVATCALDHAQPLLEHLQPKLATVNDLACRGLDKLEEKLPFLQQPSDTVVTSAKDAVAKS
  VTGVVDLAQRGRRWSGELRRSVSQAMDTVLRRSVSQAMDTVLGKSEELVDHFLPMTEAELVALATESQGP
  EVGSVEEQRQKQGYFVRLGSLSARLRHLAYQHSLGKLRESKHRTQEMLAQLQKTLELIQHMQSRASPTPT
  FHHPKVQELCGDWSPCLENGYRHSQVELETLALSRSLTLELQSAVDALAGCVRGLPPSAQAKVAEVQRSV
  DALQATFADAHCLGDVAPTALAEGQDSVAQAHACVDEFLDLVLRAMPLAWLVGPFAPILVERSEPLINLA
  TCVDEVVGDPDPRWAHMDWPAQQRAWEAEPADPGGQEAEPPLGQVKHTMMPELDF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001134637
• ORF Size: 1425 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001134637.1, NP_001128109.1
• RefSeq Size: 1937 bp
• RefSeq ORF: 1428 bp
• Locus ID: 501283
• UniProt ID: M0R7Z9
• Cytogenetics: 9q12
• MW: 51.8 kDa
• Gene Summary: Lipid droplet-associated protein that maintains the balance between lipogenesis and lipolysis and also regulates fatty acid oxidation in oxidative tissues. Recruits mitochondria to the surface of lipid droplets and is involved in lipid droplet homeostasis by regulating both the storage of fatty acids in the form of triglycerides and the release of fatty acids for mitochondrial fatty acid oxidation. In lipid droplet triacylglycerol hydrolysis, plays a role as a scaffolding protein for three major key lipolytic players: ABHD5, PNPLA2 and LIPE. Reduces the triacylglycerol hydrolase activity of PNPLA2 by recruiting and sequestering PNPLA2 to lipid droplets. Phosphorylation by PKA enables lipolysis probably by promoting release of ABHD5 from the perilipin scaffold and by facilitating interaction of ABHD5 with PNPLA2. Also increases lipolysis through interaction with LIPE and upon PKA-mediated phosphorylation of LIPE.[UniProtKB/Swiss-Prot Function]