Hsd11b1 (NM_017080) Rat Tagged ORF Clone

CAT#: RR201081

Hsd11b1 (Myc-DDK-tagged ORF) - Rat hydroxysteroid 11-beta dehydrogenase 1 (Hsd11b1), (10 ug)

Product Images

Circular map for RR201081

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hsd11b1
• Synonyms: LRRGT00065
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR201081 representing NM_017080
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAAAATACCTCCTCCCCGTCCTGGTGCTCTGCCTGGGTTACTACTATTCTACAAATGAAGAGTTCA
  GACCAGAAATGCTCCAGGGGAAGAAAGTGATTGTCACTGGGGCCAGCAAAGGGATCGGAAGAGAAATGGC
  ATATCATCTGTCAAAAATGGGAGCCCATGTGGTATTGACTGCAAGGTCGGAGGAAGGGCTCCAGAAGGTG
  GTGTCTCGCTGCCTTGAACTCGGAGCAGCCTCTGCTCACTATATTGCCGGCACTATGGAAGACATGGCTT
  TTGCAGAGCGATTTGTTGTTGAGGCAGGAAAGCTCTTGGGTGGACTGGACATGCTCATTCTCAATCACAT
  CACACAGACCACTATGTCTCTCTTCCACGATGATATCCACTCTGTGCGAAGAAGCATGGAGGTCAACTTC
  CTCAGCTATGTGGTCCTGAGCACAGCAGCCTTGCCCATGCTGAAACAGAGCAATGGCAGCATTGCCATCA
  TCTCCTCCATGGCTGGGAAAATGACCCAACCTCTGATTGCTTCCTACTCTGCAAGCAAGTTTGCTCTGGA
  TGGGTTCTTTTCCACCATTAGAAAAGAACACTTGATGACCAAGGTCAACGTGTCCATCACTCTCTGTGTC
  CTCGGCTTCATAGACACAGAAACAGCTTTGAAAGAAACCTCTGGGATAATCTTGAGTCAAGCTGCTCCCA
  AGGAGGAATGCGCCCTGGAGATCATCAAAGGCACAGTTCTGCGCAAAGATGAGGTATACTATGACAAATC
  ATCTTGGACTCCACTTCTGCTTGGGAATCCAGGAAGGAGGATCATGGAATTTCTTTCATTACGGTCATAT
  AACAGGGACCTATTTGTAAGCAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR201081 representing NM_017080
  Red=Cloning site Green=Tags(s)
  
  MKKYLLPVLVLCLGYYYSTNEEFRPEMLQGKKVIVTGASKGIGREMAYHLSKMGAHVVLTARSEEGLQKV
  VSRCLELGAASAHYIAGTMEDMAFAERFVVEAGKLLGGLDMLILNHITQTTMSLFHDDIHSVRRSMEVNF
  LSYVVLSTAALPMLKQSNGSIAIISSMAGKMTQPLIASYSASKFALDGFFSTIRKEHLMTKVNVSITLCV
  LGFIDTETALKETSGIILSQAAPKEECALEIIKGTVLRKDEVYYDKSSWTPLLLGNPGRRIMEFLSLRSY
  NRDLFVSN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017080
• ORF Size: 864 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017080.2, NP_058776.2
• RefSeq Size: 1585 bp
• RefSeq ORF: 867 bp
• Locus ID: 25116
• UniProt ID: P16232
• Cytogenetics: 13q27
• MW: 31.9 kDa
• Gene Summary: catalyzes the interconversion of cortisol and cortisone; plays a role in glucocorticoid metabolism; may regulate blood pressure [RGD, Feb 2006]