Habp4 (NM_001079940) Rat Tagged ORF Clone

CAT#: RR201069

Habp4 (Myc-DDK-tagged ORF) - Rat hyaluronan binding protein 4 (Habp4), (10 ug)

Product Images

Circular map for RR201069

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Habp4
• Synonyms: IHABP-4; IHABP4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR201069 representing NM_001079940
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGGGGCCCTGGGGAGCCCGGTAGCCGCCGCGGGCGCCGCGATGCAGGAGACGTTCGGCTGCGTCG
  TGGCCAACCGCTTCCATCAGCTGCTGGACGACGAGTCGGACCCGTTCGACATCCTGCGGGAGGCCGAGCA
  CCGGCGCCAGCAGCAGCTGCAGCGCAAGCGTCGCGATGAGGCGGCGGCGGCCAGCGGGGCTGGGCACCGT
  GGCGGCAGGAGCCCTGCCGTGGCCTCAGGACACAGGCCCGGCGCGGCCGGCCGCAGGGAGTCGCAGAAGG
  AGCGCAAGAGCCTCGCGGTGTCCAGCGCACAGCAGCCAGACAGCCCCGGGGGCCCTCAGCCGCCAGGCCA
  GAAGCGGACTCCTAGAAGAGGGGAGCAGCAAGGATGGAATGACAACAGGGGGACAGACGTGGTGCTTGAT
  AGAGCAGAGCGAAGGTCCTACAGGGAATACCGGCCCTACGACACTGAGAGACAGGCCGAGTCGACAGCGG
  AGAAGTTTACTGACGAAAAACCAGTTGACAGGTTTGATCGAGACAGACCACTGAGAGGACGTGGAGGCCC
  CAGAGGAGGCCTGAGGAACAGAGGCCGAGGTGGTCCTGGGAACAGAGCCTTTGACTCCTTTGACCAGAGA
  GGGAAACGAGATTTTGAAAGATATGGTAGCAGTGATAAAGCAAACAGAATGGAGGACAGTATGGGTGGCT
  GTGGCGTTCGCACCTGGGGATCAGGTAAAGACACTAGTGACACAGAGCCACCTGCACCCATGGAAGAGAC
  CTCGATGATGGAGGAGTGCCAGGGCGTCCTGGACGAGGAGTCTGCATCCAAAGTCCCTGAGTTGGAGGTG
  GAAGAGGAAAATCAAGTCCAAGAGATGACCTTAGATGAGTGGAAAAACCTTCAAGAACAAACCAGACCAA
  AGCCTGAGTTCAATATCCGGAAGCCAGAGTCCACAGTTCCTTCCAAGGCAGTGGTGATTCACAAGTCCAG
  ATACAGAGATGATATTGTGAAGGATGACTATGAGGATGAGTCTCACGTCTTCCGGAAAGCTGCCAATGAT
  ATCACCTCCCAGCTGGAGATTAACTTTGGTAACCTCCCTCGCCCTGGACGGGGAGCCAGAGGCAGCACCC
  GGGGAGGCCGAGGAAGAATCAGAAGAACGGAGAACTATGGCCCCCGAGCAGAAGTGGTGACCCAAGATGT
  TGCTCCCAACCCGGATGACCCTGAGGACTTCCCCGCCCTGGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR201069 representing NM_001079940
  Red=Cloning site Green=Tags(s)
  
  MKGALGSPVAAAGAAMQETFGCVVANRFHQLLDDESDPFDILREAEHRRQQQLQRKRRDEAAAASGAGHR
  GGRSPAVASGHRPGAAGRRESQKERKSLAVSSAQQPDSPGGPQPPGQKRTPRRGEQQGWNDNRGTDVVLD
  RAERRSYREYRPYDTERQAESTAEKFTDEKPVDRFDRDRPLRGRGGPRGGLRNRGRGGPGNRAFDSFDQR
  GKRDFERYGSSDKANRMEDSMGGCGVRTWGSGKDTSDTEPPAPMEETSMMEECQGVLDEESASKVPELEV
  EEENQVQEMTLDEWKNLQEQTRPKPEFNIRKPESTVPSKAVVIHKSRYRDDIVKDDYEDESHVFRKAAND
  ITSQLEINFGNLPRPGRGARGSTRGGRGRIRRTENYGPRAEVVTQDVAPNPDDPEDFPALA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001079940
• ORF Size: 1233 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001079940.1, NP_001073409.1
• RefSeq Size: 1451 bp
• RefSeq ORF: 1236 bp
• Locus ID: 361196
• UniProt ID: A1L1K8
• Cytogenetics: 17p14
• MW: 45.8 kDa
• Gene Summary: RNA-binding protein that plays a role in the regulation of transcription, pre-mRNA splicing and mRNA translation (PubMed:15862299). Negatively regulates DNA-binding activity of the transcription factor MEF2C in myocardial cells in response to mechanical stress (PubMed:15862299). Plays a role in pre-mRNA splicing regulation. Binds (via C-terminus) to poly(U) RNA. Involved in mRNA translation regulation, probably at the initiation step (By similarity). Seems to play a role in PML-nuclear bodies formation (By similarity).[UniProtKB/Swiss-Prot Function]