L1cam (NM_017345) Rat Tagged ORF Clone

CAT#: RR200937

L1cam (Myc-DDK-tagged ORF) - Rat L1 cell adhesion molecule (L1cam), (10 ug)

Product Images

Circular map for RR200937

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L1cam
• Synonyms: Hsas; Hyd; N-CAM L1; NCAML1; NgCAM
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200937 representing NM_017345
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCATGATGCTGTGGTACGTGTTGCCTCTTCTCCTCTGCAGCCCCTGCCTGCTCATACAGATTCCTG
  ATGAATATAAAGGACACCATGTACTGGAGCCACCTGTCATCACAGAACAGTCTCCACGGCGCCTGGTTGT
  CTTCCCAACAGATGACATAAGCCTCAAATGTGAAGCCAGAGGCAGACCCCAAGTGGAGTTCCGCTGGACG
  AAAGATGGCATCCACTTCAAACCTAAGGAAGAATTGGGTGTAGTGGTACACGAGGCACCCTATTCTGGCT
  CCTTCACCATCGAAGGCAACAACAGCTTTGCCCAGAGGTTTCAGGGCATCTATCGCTGCTATGCCAGCAA
  TAATCTAGGAACTGCCATGTCGCATGAGATCCAGCTCGTGGCTGAGGGTGCCCCCAAATGGCCGAAGGAG
  ACTGTAAAACCCGTGGAAGTGGAGGAAGGAGAATCAGTAGTTCTACCTTGCAATCCTCCACCCAGTGCAG
  CCCCACTTAGGATCTACTGGATGAACAGCAAGATTTTGCACATCAAACAAGATGAGCGGGTGTCCATGGG
  CCAGAACGGAGACCTATATTTTGCCAATGTGCTTACCTCAGACAATCATTCAGACTACATCTGCAATGCC
  CACTTCCCTGGCACCCGGACCATCATTCAAAAGGAACCTATTGACCTCCGGGTCAAGCCCACCAACAGCA
  TGATTGACCGGAAGCCACGCCTGCTCTTCCCCACAAACTCCAGCAGTCACCTCGTGGCCTTGCAGGGCCA
  GTCATTAATCCTGGAGTGCATTGCTGAGGGATTCCCTACACCCACCATCAAGTGGCTGCACCCCAGTGAC
  CCTATGCCAACAGACCGTGTTATCTACCAGAACCATAACAAGACACTGCAGCTCCTCAATGTGGGCGAGG
  AAGATGATGGCGAGTATACCTGCCTTGCTGAGAACTCACTGGGCAGTGCTCGGCATGCCTACTATGTCAC
  TGTGGAAGCTGCCCCATACTGGCTGCAGAAGCCCCAGAGTCATTTGTATGGGCCAGGAGAGACTGCCCGC
  CTAGACTGCCAAGTCCAGGGCAGGCCCCAACCAGAGGTCACCTGGAGAATCAACGGAATGTCTATAGAGA
  AGGTGAACAAGGACCAGAAGTACCGGATTGAGCAGGGGTCTTTGATCCTGAGTAATGTGCAACCAAGTGA
  CACAATGGTGACCCAGTGTGAAGCTCGCAACCAGCATGGGCTCCTACTAGCCAATGCCTATATCTATGTT
  GTCCAGCTGCCAGCCAGGATCCTAACAAAAGACAATCAGACATACATGGCAGTAGAGGGCAGTACTGCTT
  ACTTGCTGTGCAAAGCCTTTGGAGCTCCTGTTCCCAGTGTCCAGTGGCTGGATGAGGAAGGAACCACAGT
  GCTTCAGGATGAAAGATTTTTCCCCTATGCCAATGGACACCTGGGCATCAGAGATCTCCAGGCCAATGAC
  ACTGGACGCTATTTCTGCCAGGCTGCCAATGACCAGAACAATGTGACCATTTTGGCTAACCTACAGGTTA
  AAGAAGCAACCCAGATCACACAAGGACCCCGGAGCACAATTGAGAAGAAAGGTGCAAGGGTGACATTCAC
  GTGCCAGGCCTCCTTTGACCCCTCTTTACAAGCCAGCATCACTTGGCGTGGAGATGGGAGAGACCTCCAG
  GAACGTGGAGACAGTGACAAGTATTTCATAGAAGATGGGCAACTTGTCATCAAGAGCCTGGACTACAGTG
  ACCAGGGAGACTACAGTTGTGTGGCCAGCACTGAACTGGATGAGGTGGAGAGCAGGGCACAACTCTTAGT
  GGTGGGAAGCCCTGGGCCAGTGCCTCACCTGGAGCTGTCCGACCGCCACTTGCTGAAGCAGAGCCAGGTG
  CACTTGTCTTGGAGCCCTGCTGAAGACCACAACTCTCCCATTGAGAAATATGACATTGAATTTGAGGACA
  AGGAAATGGCTCCTGAGAAATGGTTCAGTCTAGGCAAGGTGCCAGGAAATCAGACCTCTACTACCCTCAA
  GCTGTCCCCCTATGTCCACTACACCTTTCGGGTCACTGCCATTAACAAATATGGTCCTGGAGAACCCAGC
  CCTGTCTCTGAGACTGTAGTCACACCTGAGGCAGCCCCAGAGAAGAACCCTGTGGATGTGAGAGGGGAAG
  GAAATGAGACCAACAATATGGTCATCACATGGAAGCCCCTTCGGTGGATGGATTGGAATGCCCCCCAGAT
  TCAGTACCGTGTACAGTGGCGACCACTGGGCAAACAAGAGACCTGGAAGGAACAGACCGTGAGCGACCCC
  TTCCTGGTGGTGTCTAACACTTCCACATTTGTGCCTTATGAGATCAAAGTCCAGGCAGTGAACAACCAGG
  GGAAGGGCCCTGAGCCCCAGGTCACCATTGGCTATTCAGGGGAAGACTACCCCCAGGTGAGCCCTGAGCT
  TGAAGACATCACAATCTTCAACTCAAGCACTGTGCTGGTCAGGTGGAGGCCTGTGGACTTGGCCCAGGTT
  AAGGGCCACCTCAGGGGATACAATGTAACGTACTGGTGGAAGGGCAGTCAGAGAAAGCACAGCAAGAGGC
  ATGTCCACAAAAGTCACATGGTGGTACCTGCGAACACCACCAGTGCCATCCTCAGTGGTTTGCGTCCTTA
  CAGCTCTTATCATGTAGAGGTACAGGCCTTTAATGGGCGGGGCTTAGGGCCTGCAAGTGAATGGACCTTC
  AGCACCCCAGAGGGAGTGCCTGGCCACCCTGAGGCATTACATCTGGAGTGCCAGTCGGACACTAGCCTGC
  TACTGCACTGGCAGCCACCACTCAGCCACAATGGAGTGCTCACTGGCTACCTGCTCTCTTACCATCCCTT
  GGATGGGGAAAGCAAAGAGCAGTTGTTCTTCAACCTTTCGGACCCAGAGCTCCGGACTCATAATCTCACC
  AACCTCAACCCTGATCTACAGTACCGCTTCCAGCTTCAGGCCACCACCCATCAGGGTCCTGGTGAGGCCA
  TTGTGCGTGAAGGAGGCACTATGGCCCTATTTGGCAAGCCAGATTTTGGCAACATTTCAGTCACAGCAGG
  TGAAAACTACAGTGTGGTCTCCTGGGTCCCTCGGGAGGGCCAGTGCAATTTCAGGTTCCACATCCTGTTC
  AAAGCCTTGCCAGAAGGGAAAGTGAGCCCTGATCACCAGCCTCAGCCTCAATATGTGAGCTACAATCAGA
  GCTCCTACACACAGTGGGACCTACAGCCTGACACCAAATATGAGATCCACCTGATGAGGGAGAAGGTCCT
  CTTGCACCATCTGGCTGTGAAGACTAATGGCACTGGCCCCGTGCGAGTTTCTACTACAGGTAGCTTTGCC
  TCCGAGGGCTGGTTCATCGCCTTTGTCAGTGCTATCATTCTCTTGCTCCTCATCCTGCTCATCCTCTGCT
  TCATCAAACGCAGCAAGGGCGGCAAATATTCAGTGAAGGACAAGGAGGACACTCAGGTAGATTCCGAGGC
  CCGGCCCATGAAAGACGAGACCTTCGGCGAGTACAGTGACAATGAAGAGAAGGCCTTCGGCAGCAGCCAG
  CCATCTCTCAATGGAGACATCAAACCCCTAGGCAGTGATGACAGTCTGGCTGATTATGGGGGCAGTGTGG
  ATGTCCAGTTCAATGAGGATGGCTCTTTCATCGGCCAATACAGTGGCAAAAAAGAGAAGGAGGCAGCGGG
  AGGCAATGACAGCTCAGGGGCTACCTCTCCTATCAATCCTGCAGTAGCCCTAGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200937 representing NM_017345
  Red=Cloning site Green=Tags(s)
  
  MVMMLWYVLPLLLCSPCLLIQIPDEYKGHHVLEPPVITEQSPRRLVVFPTDDISLKCEARGRPQVEFRWT
  KDGIHFKPKEELGVVVHEAPYSGSFTIEGNNSFAQRFQGIYRCYASNNLGTAMSHEIQLVAEGAPKWPKE
  TVKPVEVEEGESVVLPCNPPPSAAPLRIYWMNSKILHIKQDERVSMGQNGDLYFANVLTSDNHSDYICNA
  HFPGTRTIIQKEPIDLRVKPTNSMIDRKPRLLFPTNSSSHLVALQGQSLILECIAEGFPTPTIKWLHPSD
  PMPTDRVIYQNHNKTLQLLNVGEEDDGEYTCLAENSLGSARHAYYVTVEAAPYWLQKPQSHLYGPGETAR
  LDCQVQGRPQPEVTWRINGMSIEKVNKDQKYRIEQGSLILSNVQPSDTMVTQCEARNQHGLLLANAYIYV
  VQLPARILTKDNQTYMAVEGSTAYLLCKAFGAPVPSVQWLDEEGTTVLQDERFFPYANGHLGIRDLQAND
  TGRYFCQAANDQNNVTILANLQVKEATQITQGPRSTIEKKGARVTFTCQASFDPSLQASITWRGDGRDLQ
  ERGDSDKYFIEDGQLVIKSLDYSDQGDYSCVASTELDEVESRAQLLVVGSPGPVPHLELSDRHLLKQSQV
  HLSWSPAEDHNSPIEKYDIEFEDKEMAPEKWFSLGKVPGNQTSTTLKLSPYVHYTFRVTAINKYGPGEPS
  PVSETVVTPEAAPEKNPVDVRGEGNETNNMVITWKPLRWMDWNAPQIQYRVQWRPLGKQETWKEQTVSDP
  FLVVSNTSTFVPYEIKVQAVNNQGKGPEPQVTIGYSGEDYPQVSPELEDITIFNSSTVLVRWRPVDLAQV
  KGHLRGYNVTYWWKGSQRKHSKRHVHKSHMVVPANTTSAILSGLRPYSSYHVEVQAFNGRGLGPASEWTF
  STPEGVPGHPEALHLECQSDTSLLLHWQPPLSHNGVLTGYLLSYHPLDGESKEQLFFNLSDPELRTHNLT
  NLNPDLQYRFQLQATTHQGPGEAIVREGGTMALFGKPDFGNISVTAGENYSVVSWVPREGQCNFRFHILF
  KALPEGKVSPDHQPQPQYVSYNQSSYTQWDLQPDTKYEIHLMREKVLLHHLAVKTNGTGPVRVSTTGSFA
  SEGWFIAFVSAIILLLLILLILCFIKRSKGGKYSVKDKEDTQVDSEARPMKDETFGEYSDNEEKAFGSSQ
  PSLNGDIKPLGSDDSLADYGGSVDVQFNEDGSFIGQYSGKKEKEAAGGNDSSGATSPINPAVALE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017345
• ORF Size: 3765 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017345.1, NP_059041.1
• RefSeq Size: 5093 bp
• RefSeq ORF: 3768 bp
• Locus ID: 50687
• UniProt ID: Q05695
• Cytogenetics: Xq37
• MW: 140.4 kDa
• Gene Summary: member of the Ig superfamily of cell adhesion molecules; involved in neuronal cell development; neural cell adhesion molecule expressed in brain; isoform L1cs is a likely alternative splice variant expressed in the peripheral nervous system [RGD, Oct 2007]