Gnai1 (NM_013145) Rat Tagged ORF Clone

CAT#: RR200923

Gnai1 (Myc-DDK-tagged ORF) - Rat guanine nucleotide binding protein (G protein), alpha inhibiting 1 (Gnai1), (10 ug)

Product Images

Circular map for RR200923

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gnai1
• Synonyms: BPGTPB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200923 representing NM_013145
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTGCACACTGAGCGCTGAGGACAAGGCGGCCGTGGAGCGCAGCAAGATGATCGACCGCAACCTCC
  GGGAGGACGGAGAGAAGGCAGCGCGCGAGGTCAAGCTGCTGCTGCTGGGTGCTGGTGAATCCGGGAAGAG
  CACAATTGTGAAGCAGATGAAAATTATCCACGAGGCTGGCTACTCAGAGGAAGAGTGTAAGCAGTACAAA
  GCAGTGGTCTACAGCAACACCATCCAGTCCATCATTGCCATCATTAGAGCCATGGGGAGATTGAAAATCG
  ACTTTGGAGACGCTGCTCGTGCGGATGATGCTCGCCAACTCTTCGTGCTTGCTGGGGCTGCAGAGGAAGG
  CTTTATGACCGCGGAGCTCGCCGGCGTCATAAAGAGACTGTGGAAGGACAGCGGTGTGCAAGCCTGCTTC
  AACAGATCCCGGGAGTACCAGCTGAACGATTCGGCGGCGTACTACCTGAATGACTTGGACAGAATAGCAC
  AACCAAATTACATCCCAACCCAGCAGGATGTTCTCAGAACTAGAGTGAAAACGACGGGAATTGTGGAAAC
  CCACTTTACTTTCAAAGATCTTCATTTTAAAATGTTTGACGTGGGAGGCCAGAGATCAGAGCGGAAGAAG
  TGGATTCACTGCTTTGAAGGCGTGACTGCCATCATCTTCTGTGTGGCCCTGAGTGACTATGACCTGGTTC
  TTGCTGAGGATGAAGAAATGAACCGGATGCATGAAAGCATGAAGCTGTTCGATAGCATATGTAACAACAA
  GTGGTTTACGGACACATCCATCATCCTTTTCCTGAACAAGAAGGACCTCTTCGAAGAGAAGATCAAAAAG
  AGTCCCCTCACGATATGCTATCCAGAATATGCAGGCTCAAACACATATGAAGAGGCGGCTGCGTATATCC
  AGTGTCAGTTTGAAGACCTCAATAAAAGGAAGGACACAAAGGAAATTTACACCCACTTCACTTGCGCCAC
  GGATACGAAGAATGTGCAGTTTGTGTTCGATGCTGTAACGGACGTCATCATAAAGAATAACCTAAAAGAC
  TGTGGTCTCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200923 representing NM_013145
  Red=Cloning site Green=Tags(s)
  
  MGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEAGYSEEECKQYK
  AVVYSNTIQSIIAIIRAMGRLKIDFGDAARADDARQLFVLAGAAEEGFMTAELAGVIKRLWKDSGVQACF
  NRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKK
  WIHCFEGVTAIIFCVALSDYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKK
  SPLTICYPEYAGSNTYEEAAAYIQCQFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKD
  CGLF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013145
• ORF Size: 1062 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013145.1, NP_037277.1
• RefSeq Size: 1945 bp
• RefSeq ORF: 1065 bp
• Locus ID: 25686
• UniProt ID: P10824
• Cytogenetics: 4q11
• MW: 40.3 kDa
• Gene Summary: plays a role in presynaptic inhibition and regulation of synaptic transmission [RGD, Feb 2006]