Zp1 (NM_053509) Rat Tagged ORF Clone

CAT#: RR200920

Zp1 (Myc-DDK-tagged ORF) - Rat zona pellucida glycoprotein 1 (sperm receptor) (Zp1), (10 ug)

Product Images

Circular map for RR200920

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Zp1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200920 representing NM_053509
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTGGGGTTGTTTTGTGGTCCTGCTTCTGCTGGTGGCAGCTCCCCTGAGATTGGGTCAGCATCTGC
  ATCTCAAGCCTGGCTTTCAATACAGCTATGACTGTGGGGTACAGGGCATGCAGCTGCTGGTGTTCCCCAG
  GCCAAACCAGACTATCCAATTCAAGGTGCTGGATGAATTTGGGAACCGGTTTGAGGTGAATAACTGCTCT
  ATCTGCTACCACTGGGTGATCAGTGAGGCCCAGAAGCCCGCAGTGTTCTCAGCTGATTACAAAGGTTGCC
  ACGTGCTGGAGAAGCAGGATGGGCGTTTCCACCTGAGGGTGTTCATACAAGCTGTACTACCCAATGGCCG
  TGTGGATACAGCACAAGATGTCACTCTGATCTGTCCCAAACCAGACCACATCTTGACTCCAGAGTCCTAC
  CTGGCACCACCCACCACACCTCAGCCTTTTATACCTCATACTTTTGCCCTTCACCCCATCTCTGGCCACA
  CCTTGGCTGGGTCTGGCCACACTGGTCTCACTACATTGTACCCAGAGACCCATCCAACTCCCGCTCCACC
  ATCCTCAGAACCTGGACCTGTTGGACCCACCGTGCCTCAGTCCCAGTGGGGCACCTTGGGGTCCTGGGAA
  TTGACTGAGCTTGATTCTATAGGTACCCATCTGCTCCAGGAGCGGTGTCAGGTAGCCTCTGGGCACATCC
  CCTGCATGGTAAAAGGTAGTTCCGAGGAAGCCTGTCAGCAGGCTGGCTGCTGCTATGACAATACCAAAGA
  AATGCCCTGTTACTATGGCAACACAGTCACTCTCCAGTGTTTCAGAAGTGGCTACTTTACCTTGGTCATG
  TCGCAAGAAACAGCCTTGACACACGGGGTCATGCTGGACAATGTCCACCTAGCCTATGCCCCCAACGGAT
  GCCCCCCTACCCAGAAGACAAGTGCTTTTGTGGTTTTCCATGTCCCTCTCACCCTCTGTGGAACAGCAAT
  CCAGGTGGTTGGTAAGCAGCTCGTCTATGAGAACCAGCTGGTGTCTAACATAGAGGTCCAAACGGGACCG
  CAGGGTTCCATCACTCGGGATGGTGTCTTCCGGCTTCATGTTCGCTGTATCTTCAATGCTAGTGATTTCC
  TGCCCATCCGGGCATCTATCTTCTCACCACAACCACCTGCCCCTGTGACCCGGTCTGGACCCCTGAGGCT
  GGAGCTGAGGATTGCCACGGATAAGACTTTCAGCTCCTACTACCAGGGGAGTGACTATCCCCTTGTGAGA
  CTGCTCCAGGAACCAGTTTACATAGAGGTCCGTCTCCTGCAGAGAACTGATCCTGGTCTGGCTCTCATGC
  TTCATCAGTGCTGGGCCACACCCTCTGCCAGCCCCTTTGAGCAGCCCCAATGGCCCATTCTGTCAGATGG
  GTGTCCTTTCAAGGGTGACAACTACAGAACACAAATGGTGGCTGCAGACAGGGCAACGCTGCCCTTCTGG
  TCTCACTATCAGCGCTTCACCATTGCCACCTTCACTCTCCTTGACTCCAGCTCCCAGAATGCCCTTCGGG
  GACAGGTCTATTTCTTCTGTAGTGCCTCTGCCTGCCACCCTGTGGGGTCCGAGACGTGCTCTACCACATG
  TGATTCTGAGATAGCAAGGCATCGACGATCCTCTGGTCACCACAATAGCACTATCCGGGCCCTGGACATT
  GTGAGCTCTCCAGGAGCAGTGGGCTTTGAGGATGCTCCTAAACTGGAGCCTTCAGGTTCCACCAGGAACT
  CCGGTTCAAGACCCTTACTCTGGGTGCTCCAACTGCTGGCCCTCACCCTAGTCCTGGGGGATGGTGTCTT
  GGTGGGTCTGAGCTGGGCCTGGGCCTGGGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200920 representing NM_053509
  Red=Cloning site Green=Tags(s)
  
  MAWGCFVVLLLLVAAPLRLGQHLHLKPGFQYSYDCGVQGMQLLVFPRPNQTIQFKVLDEFGNRFEVNNCS
  ICYHWVISEAQKPAVFSADYKGCHVLEKQDGRFHLRVFIQAVLPNGRVDTAQDVTLICPKPDHILTPESY
  LAPPTTPQPFIPHTFALHPISGHTLAGSGHTGLTTLYPETHPTPAPPSSEPGPVGPTVPQSQWGTLGSWE
  LTELDSIGTHLLQERCQVASGHIPCMVKGSSEEACQQAGCCYDNTKEMPCYYGNTVTLQCFRSGYFTLVM
  SQETALTHGVMLDNVHLAYAPNGCPPTQKTSAFVVFHVPLTLCGTAIQVVGKQLVYENQLVSNIEVQTGP
  QGSITRDGVFRLHVRCIFNASDFLPIRASIFSPQPPAPVTRSGPLRLELRIATDKTFSSYYQGSDYPLVR
  LLQEPVYIEVRLLQRTDPGLALMLHQCWATPSASPFEQPQWPILSDGCPFKGDNYRTQMVAADRATLPFW
  SHYQRFTIATFTLLDSSSQNALRGQVYFFCSASACHPVGSETCSTTCDSEIARHRRSSGHHNSTIRALDI
  VSSPGAVGFEDAPKLEPSGSTRNSGSRPLLWVLQLLALTLVLGDGVLVGLSWAWAWA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053509
• ORF Size: 1851 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053509.1, NP_445961.1
• RefSeq Size: 1947 bp
• RefSeq ORF: 1854 bp
• Locus ID: 85271
• UniProt ID: O54766
• Cytogenetics: 1q43
• MW: 67.9 kDa
• Gene Summary: glycoprotein component of the zona pellucida [RGD, Feb 2006]