Gfra3 (NM_053398) Rat Tagged ORF Clone

CAT#: RR200905

Gfra3 (Myc-DDK-tagged ORF) - Rat GDNF family receptor alpha 3 (Gfra3), (10 ug)

Product Images

Circular map for RR200905

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gfra3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200905 representing NM_053398
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCTCTCCCGGAGCCCGCGACCGCCGCCGCTAGTGATCCTGCTACTGGTGCTGTCGCTGTGGCTAC
  CCCTTGGAACAGGAAACTCCCTTCCCACAGAGAACAGGCTTGTGAACAGCTGTACCCAGGCCAGAAAAAA
  ATGCGAGGCTAATCCCGCTTGCAAGGCTGCCTACCAGCACCTGGACTCCTGCACCCCCAGTCTCAGCAGT
  CCACTGCCCTCAGGGGAGTCTGCCACATCTGCAGCGTGCCTTGAAGCAGCACAGCAACTCAGGAACAGCT
  CTCTCATAGACTGCAGGTGCCACCGGCGCATGAAGCACCAAGCTACCTGTCTGGACATTTATTGGACCGT
  TCACCCTGTCCGAAGCCTTGGTGACTACGAGTTGGACGTCTCACCCTATGAAGACACAGTGACCAGCAAA
  CCCTGGAAAATGAATCTCAGCAAGCTGAGCATGCTCAAACCAGACTCCGACCTCTGCCTCAAATTTGCTA
  TGCTGTGTACTCTTAACGACAAGTGCGACCGCCTCCGAAAGGCCTACGGGGAGGCGTGCTCAGGGATCCG
  CTGCCAGCGCCACCTCTGCCTAGCTCAGCTGCGCTCCTTCTTCGAGAAGGCGGCAGAGTCCCACGCTCAG
  GGCCTGCTGCTGTGTCCCTGTGCACCCGAAGATGCGGGCTGTGGGGAGCGCCGGCGCAACACCATCGCCC
  CCAGTTGCGCCCTCCCGTCTGTGGCCCCCAACTGCCTAGATCTTCGGAGCTTCTGCCGTGCGGACCCTCT
  GTGCAGATCACGCCTGATGGACTTCCAGACCCACTGCCACCCTATGGACATCCTCGGGACTTGTGCAACT
  GAGCAGTCCAGATGTCTGCGGGCATACCTGGGGCTAATTGGGACTGCCATGACCCCAAACTTCATCAGCA
  AGGTCAACACTACTGTTGCCTTAGGCTGTACCTGCCGAGGCAGTGGCAACCTGCAGGACGAGTGTGAACA
  GCTGGAAAAGTCCTTCTCCCAGAACCCCTGCCTCATGGAGGCCATTGCGGCTAAAATGCGTTTCCACAGA
  CAACTCTTCTCCCAGGACTGGGCGGACTCTACTTTTTCTGTGATGCAGCAGCAGAACAGCAGCCCTGCTC
  TGAGGCCCCAGCTCAGGCTACCCGTTCTGTCTTTCTTCATCCTTACCTTGATTCTGCTGCAGACCCTCTG
  GG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200905 representing NM_053398
  Red=Cloning site Green=Tags(s)
  
  MGLSRSPRPPPLVILLLVLSLWLPLGTGNSLPTENRLVNSCTQARKKCEANPACKAAYQHLDSCTPSLSS
  PLPSGESATSAACLEAAQQLRNSSLIDCRCHRRMKHQATCLDIYWTVHPVRSLGDYELDVSPYEDTVTSK
  PWKMNLSKLSMLKPDSDLCLKFAMLCTLNDKCDRLRKAYGEACSGIRCQRHLCLAQLRSFFEKAAESHAQ
  GLLLCPCAPEDAGCGERRRNTIAPSCALPSVAPNCLDLRSFCRADPLCRSRLMDFQTHCHPMDILGTCAT
  EQSRCLRAYLGLIGTAMTPNFISKVNTTVALGCTCRGSGNLQDECEQLEKSFSQNPCLMEAIAAKMRFHR
  QLFSQDWADSTFSVMQQQNSSPALRPQLRLPVLSFFILTLILLQTLW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053398
• ORF Size: 1191 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053398.1, NP_445850.1
• RefSeq Size: 2070 bp
• RefSeq ORF: 1194 bp
• Locus ID: 84422
• Cytogenetics: 18p12
• MW: 44.1 kDa
• Gene Summary: responsible for neuronal cell protection and cell survival in the inner ear [RGD, Feb 2006]