Gpr135 (NM_181771) Rat Tagged ORF Clone

CAT#: RR200777

Gpr135 (Myc-DDK-tagged ORF) - Rat G protein-coupled receptor 135 (Gpr135), (10 ug)

Product Images

Circular map for RR200777

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gpr135
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200777 representing NM_181771
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGAGCAGGCGCGGCCGCCGAGCCGCCCGGCTGCGAGTGCAACCTTGCCGGGTAGCGCACATCCCG
  GAGGGGCGGCTTCCACGGCCACCGCGGCCGCGCTGTCGTTCAGCTCCGTAGCCACCGTAACGCTGGGAAA
  CCAGAGCGATGCGGGCAGGCCGGAGGCGGCTGGGTCTCGGGGACCGGCACCGCTGCTTTGGCACGGGGCA
  GCAGTGGCCGCCCAGGCGCTCGTGCTCCTGCTCATCTTCTTACTGTCTAGCCTGGGAAACTGCGCGGTGA
  TGGGGGTGATCGTGAAGCACCGGCAGCTGCGCACGGTCACCAACGCCTTCATCCTATCGCTGTCCCTGTC
  GGACCTGCTCACTGCGCTGCTCTGCTTACCCGCCGCCTTCCTCGACCTGTTTGCGCCGCCGGGAGACTCG
  GGGCCCTGGCGCAGCTTCTGCGCCGCCAGCCGCTTCTTCAGTTCGTGTTTCGGCATCGTCTCCACGTTCA
  GCGTGGCGCTCATCTCGCTGGACCGCTACTGCGCCATCGTGCGGCCGCCGCGGGACAAGCTAGGCCGGCG
  GCGCGCGCTGCAGCTGCTGGCCGGAGCGTGGCTGGCTGCGCTCGGCTTCTCCCTGCCCTGGGAGCTGCTC
  CGGGCACCCCGGGAGCCCCCGACTCCGCAGAGCTTCCACCGCTGCCTTTACAGAACCTCCCCAGACCCTG
  CGCAGCTGGGCGCCGCTTACAGCGTGGGGCTGGTGGTGGCTTGCTACCTGCTGCCCTTCCTGCTGATGTG
  TTTCTGCCGCTACCACATCTGCAAGACTGTGCGCCTGTCGGACGTGCGTGTGCGGCCGATGACCACCTAT
  GCGCGCGTGCTGCGCTTTTTCAGCGAGGTGCGCACGGCCACCACCGTGCTCATCATGATTGTCTTTGTCA
  TCTGCTGCTGGGGCCCCTACTGCTTCCTGGTGTTGTTGGCTGCTACCCGGCAGGGTCAGACCACACAGGC
  TCCCTCGCTGCTCAATGTGGCAGCTGTTTGGCTGACCTGGGCCAATGGAGCTATCAACCCGGTCATATAT
  GCCATCCGCAACCCTAACATTTCTATGTTCCTAGGTCGCAACCGCGAAGAGGGATATAGGACTAGAAACA
  TGGATGTTTTTTTGCCTAGCCAAGGCCTAGGTTTTCAGGCCAGAAGTCGCAATCGCCTTCGAAATGGCTG
  TGCCAACAGGCTTGGGGCTTGCAGCAGGATGCCTTCTTCCAACCCCGCTAGTGGGTCAGGAGGGGAAGTG
  GTCATGTGGGCTCGAAAAAACCCAGTTGTGCTCTTCTTCCGAGAGGATCCACCAGACCCAGTTATGGCAG
  TCTACAAACAGCATAAATCTGAAACCAGGGACAGTAGCATCTCTACAAACAGCATAAATCTGAAACCAGG
  GACAGTAGCATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200777 representing NM_181771
  Red=Cloning site Green=Tags(s)
  
  MEEQARPPSRPAASATLPGSAHPGGAASTATAAALSFSSVATVTLGNQSDAGRPEAAGSRGPAPLLWHGA
  AVAAQALVLLLIFLLSSLGNCAVMGVIVKHRQLRTVTNAFILSLSLSDLLTALLCLPAAFLDLFAPPGDS
  GPWRSFCAASRFFSSCFGIVSTFSVALISLDRYCAIVRPPRDKLGRRRALQLLAGAWLAALGFSLPWELL
  RAPREPPTPQSFHRCLYRTSPDPAQLGAAYSVGLVVACYLLPFLLMCFCRYHICKTVRLSDVRVRPMTTY
  ARVLRFFSEVRTATTVLIMIVFVICCWGPYCFLVLLAATRQGQTTQAPSLLNVAAVWLTWANGAINPVIY
  AIRNPNISMFLGRNREEGYRTRNMDVFLPSQGLGFQARSRNRLRNGCANRLGACSRMPSSNPASGSGGEV
  VMWARKNPVVLFFREDPPDPVMAVYKQHKSETRDSSISTNSINLKPGTVA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_181771
• ORF Size: 1371 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 1374 bp
• RefSeq ORF: 1374 bp
• Locus ID: 314213
• UniProt ID: Q7TQN7
• Cytogenetics: 6q24
• MW: 49.6 kDa
• Gene Summary: G protein-coupled receptor of the family of rhodopsin-type GPCRs [RGD, Feb 2006]