Plcg2 (NM_017168) Rat Tagged ORF Clone

CAT#: RR200573

Plcg2 (Myc-DDK-tagged ORF) - Rat phospholipase C, gamma 2 (Plcg2), (10 ug)

Product Images

Circular map for RR200573

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Plcg2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200573 representing NM_017168
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCACCATGGTCAACGTGGACACCCTTCCAGAATATGAGAAGAGTCAGATCAAGAGAGCCCTGGAGC
  TAGGGACGGTGATGACCGTATTCAGTGCCCGCAAATCCACCCCAGAGCGGAGGACAGTACAGATGATCAT
  GGAGACCCGGCAGGTGGCATGGAGCAAGACAGCAGATAAGATCGAAGGCTTCTTGGACATCATGGAAATA
  AAGGAAATCCGGCCAGGGAAGAACTCCAAGGACTTTGAGCGAGCCAAGGCTGTCCGTCACAAGGCAGACT
  GCTGCTTCACCATCTTCTACGGCACCCAGTTCGTGCTCAGCACTCTCAGTCTGGCAACCGACTCAAAGGA
  GGATGCAGTGAAGTGGCTCTCTGGCTTGAAGATCTTACACCAGGAAGCGATGAACGCATCCACTCCCACA
  ATGATTGAGAGTTGGCTGCGGAAACAGATTTATTCAGTAGATCAAACCCGAAGAAACAGCATCAGCCTCC
  GGGAGCTGAAGACCATCTTGCCCCTGGTCAACTTCAAAGTGAGCGGCATCAAGTTCCTCAAGGACAAGCT
  GGTGGAAATTGGCGCCCAGAAAGATGAGCTCAGTTTTGAACAGTTCCATCTCTTCTATAAAAAACTCATG
  TTTGAGCAGCAGAAGTCGATACTTGACGAATTCAAAAAAGACTCCTCCGTGTTCATCCTAGGGAACACAG
  ACCGGCCTGATGCCTCAGCCGTCTACCTGCAAGACTTCCAGAGGTTTCTTTTACATGAACAGCAGGAGCT
  ATGGGCTCAGGATCTGAACAAAGTCCGGGAGCGGATGACCAAGTTTATCGATGACACAATGAGGGAGACC
  GCAGAGCCCTTCTTGTTTGTGGATGAGTTCCTCACGTATCTGTTCTCACGGGAGAACAGCATCTGGGACG
  AGAAGTACGATGCCGTGGACATGCAGGACATGAACAACCCCTTGTCCCATTACTGGATCTCCTCTTCCCA
  CAACACGTACCTCACTGGAGACCAGCTGCGTAGCGAGTCCTCCACGGAAGCGTATATCCGCTGTCTGCGT
  GCTGGTTGTCGCTGCATCGAGTTGGATTGCTGGGATGGGCCTGACGGGAAACCCATAATCTATCACGGTT
  GGACACGGACCACCAAGATCAAGTTTGATGATGTTGTTCAGGCCATCCGGGATCATGCCTTTGTTACCTC
  CAGCTTTCCCGTGATTCTGTCTATCGAGGAGCACTGCAGTGTGGAGCAGCAACGTCACATGGCCAAGGTC
  TTCAAGGAAGTGTTAGGAGACCTGCTGTTGACGAAGCCCACGGAGGCCAGTGCTGACCAGCTGCCCTCAC
  CCAGCCAGCTTCGTGAGAAGATCATTATCAAGCATAAGAAGCTGGGCCCCCGAGGTGATGTCGATGTCAA
  TGTGGAGGACAAGAAAGATGAGCACAAGACCCAGGGCGAGCTGTATATGTGGGACTCCATCGACCAGAAA
  TGGACTCGCCACTACTGTGCTATTGCGGACGCCAAGCTGTCCTTCAGTGACGACATTGAACAGACTGTGG
  AAGAGGATCCGGTCCAGGACACTCCCCCCACGGAGCTACATTTTGGGGAGAAATGGTTCCATAAGAAGGT
  GGAGAGCAGGACCAGTGCGGAGAAGCTGCTGCAGGAGTACTGTGCCGAGACTGGGGCCAAGGATGGCACC
  TTCCTGGTGCGGGAGAGCGAGACCTTCCCCAACGACTACACACTCTCCTTCTGGCGGTCTGGCCGGGTGC
  AGCACTGCCGAATCCGGTCTACCATGGAGGGTGGGGTCATGAAGTACTACCTGACTGACAACCTCACGTT
  CAACAGCATCTACGCCCTCATCCAGCACTACCGGGAGGCACACCTGCGCTGTGCGGAGTTCGAGCTGCGG
  CTCACAGACCCGGTGCCCAACCCCAACCCACATGAGTCCAAGCCGTGGTACTATGACAGGCTGAGCAGGG
  GGGAAGCAGAGGACATGTTGATGCGGATTCCCCGAGATGGAGCCTTCCTCATTCGGAAGCGGGAGGGGAC
  CGACTCTTACGCCATCACCTTCAGGGCCAGGGGCAAGGTGAAACATTGCCGTATCAACCGGGACGGACGT
  CACTTTGTGCTGGGGACCTCTGCCTACTTTGAGAGCCTGGTGGAGCTCGTCAGCTACTATGAGAAGCACG
  CACTCTACCGCAAGATGAGGCTTCGCTACCCCGTGACCCCGGAGCTCCTGGAACGATATAATATGGAAAG
  AGACATCAACTCTCTCTATGACGTCAGCAGGATGTATGTGGACCCAAGTGAGATCAACCCTTCCATGCCT
  CAGAGGACTGTGAAAGCACTCTATGACTACAAAGCCAAGCGCAGCGATGAGCTGACCTTCTGCCGAGGTG
  CCCTCATCCACAACGTCTCCAAGGAGCCGGGAGGATGGTGGAAAGGGGACTATGGGACCCGGATCCAGCA
  GTATTTCCCATCTAACTACGTTGAAGATATCTCGGCAGGCGATGCTGAGGAGATGGAAAAGCAGATTATT
  GAAGACAACCCCCTCGGGTCTCTCTGCAGAGGCATATTGGATCTTAATACCTACAATGTGGTGAAGGCCC
  CCCAGGGGAAAAACCAGAAAGCCTTCGTCTTTATCCTGGAGCCTAAGAAACAGGGGGACCCTCCTGTGGA
  GTTCGCGACTGACCGTGTGGAGGAACTGTTTGAGTGGTTTCAGAGTATACGAGAGATCACTTGGAAGATC
  GACACCAAGGAGAACAACATGAAGTACTGGGAGAGGAATCAGTCCATTGCTATTGAGCTCTCTGACTTGG
  TTGTGTACTGCAAGCCGACTAGCAAAACCAAGGACCATTTGGAAAATCCTGACTTCCGGGAAATTCGCTC
  TTTTGTGGAGACAAAGGCAGACAGCATTGTCAGGCAAAAGCCCGTGGATCTATTGCGGTACAACCAGAAG
  GGCCTGACCCGTGTCTACCCCAAGGGACAGAGAGTTGACTCTTCCAACTACGACCCCTTCCGTCTGTGGC
  TGTGTGGCTCCCAGATGGTGGCGCTCAATTTCCAGACTCCAGACAAGTACATGCAGATGAACCATGCATT
  GTTTTCGCTCAATGGGCGGACAGGTTACGTCCTGCAGCCCGAGAGCATGCGGTCTGAGAAGTATGACCCG
  ATGCCCCCGGAGTCCCAGAGGAAGATCCTGATGACACTCACTGTCAAGGTTCTTGGTGCACGCCACCTCC
  CTAAACTAGGGCGGAGTATCGCCTGTCCCTTTGTAGAGGTGGAAATCTGTGGGGCCGAGTATGACAGCAA
  CAAATTCAAGACGACGGTTGTGAATGACAACGGTCTCAGCCCTGTCTGGGCTCCAACTCAGGAGAAGGTG
  ACATTTGAAATTTATGACCCAAACCTTGCGTTCCTACGCTTTCTGGTCTACGAAGAAGACATGTTCAGTG
  ACCCCAACTTCCTGGCTCATGCCACATACCCCATTAAAGGCATCAAATCAGGATTTAGATCAGTCCCTCT
  GAAGAACGGGTACAGTGAAGACATCGAGCTGGCATCCCTCCTGGTTTTCTGTGAGATGCGGCCAGTCCTG
  GAGAGTGAAGAAGAACTCTATTCCTCCTGTCGCCAGCTGCGGAGGCGGCAGGAAGAGCTCAACAACCAGC
  TCTTCCTGTATGACACACACCAGAACCTGCGAGGAGCCAACCGGGATGCCCTGGTGAAGGAGTTCAATGT
  TAACGAGAATCAGCTGCGGCTGTACCAGGAGAAGTGTAACCGGAGGCTGAGAGAGAAGAGAGTGAGTAAC
  AGCAGGTTCTACTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200573 representing NM_017168
  Red=Cloning site Green=Tags(s)
  
  MTTMVNVDTLPEYEKSQIKRALELGTVMTVFSARKSTPERRTVQMIMETRQVAWSKTADKIEGFLDIMEI
  KEIRPGKNSKDFERAKAVRHKADCCFTIFYGTQFVLSTLSLATDSKEDAVKWLSGLKILHQEAMNASTPT
  MIESWLRKQIYSVDQTRRNSISLRELKTILPLVNFKVSGIKFLKDKLVEIGAQKDELSFEQFHLFYKKLM
  FEQQKSILDEFKKDSSVFILGNTDRPDASAVYLQDFQRFLLHEQQELWAQDLNKVRERMTKFIDDTMRET
  AEPFLFVDEFLTYLFSRENSIWDEKYDAVDMQDMNNPLSHYWISSSHNTYLTGDQLRSESSTEAYIRCLR
  AGCRCIELDCWDGPDGKPIIYHGWTRTTKIKFDDVVQAIRDHAFVTSSFPVILSIEEHCSVEQQRHMAKV
  FKEVLGDLLLTKPTEASADQLPSPSQLREKIIIKHKKLGPRGDVDVNVEDKKDEHKTQGELYMWDSIDQK
  WTRHYCAIADAKLSFSDDIEQTVEEDPVQDTPPTELHFGEKWFHKKVESRTSAEKLLQEYCAETGAKDGT
  FLVRESETFPNDYTLSFWRSGRVQHCRIRSTMEGGVMKYYLTDNLTFNSIYALIQHYREAHLRCAEFELR
  LTDPVPNPNPHESKPWYYDRLSRGEAEDMLMRIPRDGAFLIRKREGTDSYAITFRARGKVKHCRINRDGR
  HFVLGTSAYFESLVELVSYYEKHALYRKMRLRYPVTPELLERYNMERDINSLYDVSRMYVDPSEINPSMP
  QRTVKALYDYKAKRSDELTFCRGALIHNVSKEPGGWWKGDYGTRIQQYFPSNYVEDISAGDAEEMEKQII
  EDNPLGSLCRGILDLNTYNVVKAPQGKNQKAFVFILEPKKQGDPPVEFATDRVEELFEWFQSIREITWKI
  DTKENNMKYWERNQSIAIELSDLVVYCKPTSKTKDHLENPDFREIRSFVETKADSIVRQKPVDLLRYNQK
  GLTRVYPKGQRVDSSNYDPFRLWLCGSQMVALNFQTPDKYMQMNHALFSLNGRTGYVLQPESMRSEKYDP
  MPPESQRKILMTLTVKVLGARHLPKLGRSIACPFVEVEICGAEYDSNKFKTTVVNDNGLSPVWAPTQEKV
  TFEIYDPNLAFLRFLVYEEDMFSDPNFLAHATYPIKGIKSGFRSVPLKNGYSEDIELASLLVFCEMRPVL
  ESEEELYSSCRQLRRRQEELNNQLFLYDTHQNLRGANRDALVKEFNVNENQLRLYQEKCNRRLREKRVSN
  SRFYS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017168
• ORF Size: 3795 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017168.1, NP_058864.1
• RefSeq Size: 4321 bp
• RefSeq ORF: 3798 bp
• Locus ID: 29337
• UniProt ID: P24135
• Cytogenetics: 19q12
• MW: 147.7 kDa
• Gene Summary: has phosphoinositide-specific phospholipase C activity [RGD, Feb 2006]