RGD1311084 (NM_001100979) Rat Tagged ORF Clone

CAT#: RR200552

RGD1311084 (Myc-DDK-tagged ORF) - Rat similar to 1700113K14Rik protein (RGD1311084), (10 ug)

Product Images

Circular map for RR200552

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RGD1311084
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200552 representing NM_001100979
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCCGGCGCCAGCCCATGGACAGGACCCCGGCTTACAAGCCCAAGGGGTATCCCGACGGGTCCACCC
  GACGCCGCGCCCTTCAGCAGCTACAGATGCTGCTGCCCACGCTCGACGGCCGTGCGGGGCGAGGCGCAGA
  CGGCGCGTGGTGGCGGGACCGCAAGTCCCAGCACCTGTCTTCCCCGTCCCCCGAGTCCCCGTACGCTTCG
  CGGGGCCGTGGGGCCCTGCGTGCGCTGCTGCTGCCGCCGCTGCTGCAGAGCAGCTTGACCCGGGAGGCGG
  GGGCTCGGGGGAACCGGCAGCCGGCGGACCGGGACAACCCTGACGCCCTGGGCACGCTGCTGGGGGAACT
  GCTGCCCAACAAGTTCCGCCACTTCCTTCACCAGCTGCGCGCCAAGATCGCAGAGCCAGAGGCGGACCGT
  GAGGCCGCGGGGGTGGGGTGGGGTGCAGAGTCTTGGGGTCCCAAGCGAGAACCCGCTCCATCTCACTGGG
  CTGTCCTCCCCCTTTTCCTTTCCTTCCTCCTCCCAGCACCCTCAGCGCCACAGTACTCCAGCAGTCCATC
  CGAGCACTGCCCAGCTTCTCGGTGTTCCAGCAGCTCTTTCCTCCCAGACTCGTGGGACCAACCATTGAAC
  TGGGACGACAGTTTTAGAGAGAAGACTACCCTGGGGCTCCCAAAAGGTGAATTCGTCAGAGCCAAAAAGG
  CCCATCCCCCCTCTGGAGAAGGCTCAAGACCCCGCAGACGGTATTGTCCTTTCCGGGTGCGATTCGCAGA
  CGAGACCTTGCAGGATACAGCACTCCGGTACTGGGAACGTAACCGGGCAGTCCGGCAGAATATCTTACCG
  AGCGAGCAGACGGCTTTGCCAGCTGTATCTGTGTCAGAGCGAGTGCTTGGGAGTGTTGGGAGATGGTTGG
  ACAGTCTGCCCAGAGCCCTTCAACCCAGGGTCCAAGACACCGCGGCTAGCTCCTCATGCTGGAACTGCCC
  CCGCGAGTCAGCTCAGGAACCACAGCTCTACCTTTCTGAGGATGCCACCGTGAGCAGCCGCCTACCCTTC
  ATCTCCAGGGCCACCGCCCCAAGGCCAAGGGGAGGCCTAAGAACCTTCCTGGACACCCCTAACAATCCGG
  AGCAGGTGGGCAAATCGCCCTGTCCCGGGAGCCAGAAGCTGGAATCCTTTCTGCCCAGTTTGGTGCTGCA
  GACCGTTCTAAAGCAAGGCCGCCCTAAGGGCTACCAGCTCCTTCTGCCGACCACAAACCGGCAGCAGGCT
  CACAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200552 representing NM_001100979
  Red=Cloning site Green=Tags(s)
  
  MFRRQPMDRTPAYKPKGYPDGSTRRRALQQLQMLLPTLDGRAGRGADGAWWRDRKSQHLSSPSPESPYAS
  RGRGALRALLLPPLLQSSLTREAGARGNRQPADRDNPDALGTLLGELLPNKFRHFLHQLRAKIAEPEADR
  EAAGVGWGAESWGPKREPAPSHWAVLPLFLSFLLPAPSAPQYSSSPSEHCPASRCSSSSFLPDSWDQPLN
  WDDSFREKTTLGLPKGEFVRAKKAHPPSGEGSRPRRRYCPFRVRFADETLQDTALRYWERNRAVRQNILP
  SEQTALPAVSVSERVLGSVGRWLDSLPRALQPRVQDTAASSSCWNCPRESAQEPQLYLSEDATVSSRLPF
  ISRATAPRPRGGLRTFLDTPNNPEQVGKSPCPGSQKLESFLPSLVLQTVLKQGRPKGYQLLLPTTNRQQA
  HR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001100979
• ORF Size: 1266 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001100979.1, NP_001094449.1
• RefSeq Size: 1535 bp
• RefSeq ORF: 1269 bp
• Locus ID: 311852
• Cytogenetics: 3p12
• MW: 47 kDa