Asic2 (NM_001034014) Rat Tagged ORF Clone

CAT#: RR200428

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  • TrueORF®

Asic2 (Myc-DDK-tagged ORF) - Rat amiloride-sensitive cation channel 1, neuronal (Accn1), transcript variant 2, (10 ug)



  "NM_001034014" in other vectors (3)

USD 690.00


Availability*
5 Weeks

Size
    • 10 ug


Product images

Specifications

Product Data
Product Name Asic2 (NM_001034014) Rat Tagged ORF Clone
Type Rat Tagged ORF Clone
Tag Myc-DDK
Symbol Asic2
Synonyms Accn1; BNaC1; BNC1k; MDEG; MDEG1; MDEG2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Cell Selection Neomycin
Sequence Data
>RR200428 representing NM_001034014
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACCTCAAGGAGAGCCCCAGTGAGGGCAGCCTGCAACCTTCCAGTATCCAGATCTTCGCCAATACCT
CCACTCTCCATGGCATCCGCCACATCTTCGTGTATGGGCCGCTGACCATCCGGCGTGTGCTTTGGGCAGT
GGCCTTCGTCGGATCCCTGGGCCTGCTGCTGGTGGAGAGCTCGGAGAGGGTGTCCTACTATTTCTCTTAT
CAGCATGTTACCAAGGTGGATGAAGTGGTGGCCCAGAGCCTGGTCTTCCCAGCTGTGACCCTCTGCAACC
TCAATGGCTTCCGGTTCTCCAGGCTTACCACCAACGACTTGTACCACGCTGGGGAGTTGCTGGCCCTGCT
GGATGTCAACCTACAGATTCCCGACCCGCATCTGGCAGACCCCACGGTGCTGGAGGCCCTCCGACAGAAG
GCCAACTTCAAACACTACAAACCGAAGCAGTTCAGCATGCTGGAGTTCCTGCACCGGGTAGGCCATGACC
TGAAGGATATGATGCTCTACTGCAAGTTCAAGGGGCAGGAGTGTGGGCATCAAGACTTCACCACAGTGTT
TACAAAATACGGGAAGTGTTACATGTTTAACTCAGGCGAGGATGGCAAGCCGCTGCTCACCACGGTCAAG
GGGGGGACGGGCAACGGGCTGGAGATCATGCTGGACATTCAGCAAGATGAGTACCTGCCCATCTGGGGAG
AGACAGAGGAAACAACGTTTGAAGCAGGAGTGAAGGTTCAGATCCACAGTCAGTCTGAGCCGCCTTTCAT
CCAAGAGCTGGGCTTTGGGGTGGCTCCGGGGTTCCAGACCTTCGTGGCCACACAAGAGCAGAGGCTCACA
TATCTGCCCCCACCATGGGGGGAGTGCCGGTCCTCAGAGATGGGACTCGACTTCTTTCCTGTTTACAGCA
TCACAGCCTGTCGGATTGACTGTGAGACCCGCTACATCGTGGAGAACTGTAACTGCCGCATGGTCCACAT
GCCAGGGGACGCCCCTTTCTGCACCCCTGAGCAGCACAAGGAGTGTGCAGAGCCTGCCCTCGGTCTACTG
GCAGAAAAGGACAGCAATTACTGTCTCTGCAGGACACCCTGCAACCTGACACGCTACAACAAAGAGCTCT
CCATGGTGAAGATCCCCAGCAAGACGTCAGCCAAGTACTTAGAGAAGAAATTTAACAAATCGGAAAAATA
TATCTCAGAGAACATTCTTGTTCTGGACATATTTTTTGAGGCGCTCAATTACGAAACAATTGAACAGAAG
AAGGCGTATGAAGTTGCTGCCTTACTTGGTGACATCGGTGGTCAGATGGGACTGTTCATTGGTGCTAGTC
TCCTCACAATACTAGAGCTCTTTGATTATATTTATGAGCTGATCAAAGAGAAGCTATTAGACCTGCTTGG
CAAAGAAGAAGAGGAAGGGAGCCACGATGAGAACATGAGCACCTGTGACACAATGCCAAACCACTCTGAA
ACCATCAGCCACACTGTGAACGTGCCCCTGCAGACAGCTTTGGGCACCCTGGAGGAGATTGCCTGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RR200428 representing NM_001034014
Red=Cloning site Green=Tags(s)

MDLKESPSEGSLQPSSIQIFANTSTLHGIRHIFVYGPLTIRRVLWAVAFVGSLGLLLVESSERVSYYFSY
QHVTKVDEVVAQSLVFPAVTLCNLNGFRFSRLTTNDLYHAGELLALLDVNLQIPDPHLADPTVLEALRQK
ANFKHYKPKQFSMLEFLHRVGHDLKDMMLYCKFKGQECGHQDFTTVFTKYGKCYMFNSGEDGKPLLTTVK
GGTGNGLEIMLDIQQDEYLPIWGETEETTFEAGVKVQIHSQSEPPFIQELGFGVAPGFQTFVATQEQRLT
YLPPPWGECRSSEMGLDFFPVYSITACRIDCETRYIVENCNCRMVHMPGDAPFCTPEQHKECAEPALGLL
AEKDSNYCLCRTPCNLTRYNKELSMVKIPSKTSAKYLEKKFNKSEKYISENILVLDIFFEALNYETIEQK
KAYEVAALLGDIGGQMGLFIGASLLTILELFDYIYELIKEKLLDLLGKEEEEGSHDENMSTCDTMPNHSE
TISHTVNVPLQTALGTLEEIAC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
Tag Myc-DDK
ACCN NM_001034014
ORF Size 1536 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001034014.1, NP_001029186.1
RefSeq Size 2565 bp
RefSeq ORF 1539 bp
Locus ID 25364
Cytogenetics 10q26
MW 57.7 kDa
Gene Summary member of the degenerin/epithelial Na(+) channel superfamily; permeable for Na(+), K(+) and Li(+); mutations may lead to a form of hereditary neurodegeneration; may be important for pain sensation [RGD, Feb 2006]

Citations (1)

Other Versions

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