Hnrnpd (NM_024404) Rat Tagged ORF Clone

CAT#: RR200294

Hnrpd (Myc-DDK-tagged ORF) - Rat heterogeneous nuclear ribonucleoprotein D (Hnrpd), transcript variant 1, (10 ug)

Product Images

Circular map for RR200294

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hnrnpd
• Synonyms: Auf1; Hnrpd
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200294 representing NM_024404
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGAGGAGCAGTTCGGAGGGGACGGGGCGGCGGCGGCGGCAACGGCGGCGGTAGGCGGCTCGGCGG
  GCGAGCAGGAGGGAGCCATGGTGGCGGCGGCGCAGGGGGCAGCGGCGGCGGCGGGAAGCGGGAGCGGCGG
  CGGCTCTGCGCCCGGAGGCACCGAAGGAGGCAGCACCGAGGCAGAGGGAGCGAAGATCGACGCCAGTAAG
  AATGAGGAGGATGAAGGCCATTCAAACTCCTCCCCACGACACACTGAAGCAGCGACGGCACAGCGGGAAG
  AATGGAAAATGTTTATAGGAGGCCTTAGCTGGGACACCACAAAGAAAGACCTGAAGGACTACTTTTCCAA
  ATTTGGGGACGTTGTAGACTGCACTCTGAAGTTAGATCCTATCACAGGGCGATCAAGGGGTTTTGGCTTT
  GTGCTATTTAAAGAGTCGGAGAGTGTAGATAAGGTCATGGATCAGAAAGAACATAAATTGAATGGGAAAG
  TCATTGATCCTAAAAGGGCCAAAGCCATGAAAACAAAAGAGCCCGTCAAAAAAATTTTTGTTGGTGGCCT
  TTCTCCAGACACACCTGAAGAAAAAATAAGAGAGTACTTTGGTGGTTTTGGTGAGGTTGAATCCATAGAG
  CTGCCTATGGACAACAAGACCAATAAGAGGCGTGGGTTCTGTTTCATTACCTTTAAGGAAGAGGAACCAG
  TGAAGAAGATAATGGAGAAGAAATACCACAATGTTGGTCTTAGTAAATGTGAAATAAAAGTAGCCATGTC
  GAAGGAGCAGTATCAGCAGCAGCAGCAGTGGGGATCTAGAGGAGGGTTTGCAGGAAGAGCTCGCGGAAGA
  GGCGGTGGCCCCAGTCAAAACTGGAACCAGGGATATAGTAACTATTGGAACCAAGGCTATGGCAGCTATG
  GATATAACAGCCAAGGGTACGGTGGTTATGGAGGATATGACTACACTGGTTACAACAGCTACTATGGATA
  TGGTGACTATAGCAATCAGCAGAGTGGTTATGGGAAAGTATCCAGACGAGGTGGTCATCAAAATAGCTAC
  AAACCATAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200294 representing NM_024404
  Red=Cloning site Green=Tags(s)
  
  MSEEQFGGDGAAAAATAAVGGSAGEQEGAMVAAAQGAAAAAGSGSGGGSAPGGTEGGSTEAEGAKIDASK
  NEEDEGHSNSSPRHTEAATAQREEWKMFIGGLSWDTTKKDLKDYFSKFGDVVDCTLKLDPITGRSRGFGF
  VLFKESESVDKVMDQKEHKLNGKVIDPKRAKAMKTKEPVKKIFVGGLSPDTPEEKIREYFGGFGEVESIE
  LPMDNKTNKRRGFCFITFKEEEPVKKIMEKKYHNVGLSKCEIKVAMSKEQYQQQQQWGSRGGFAGRARGR
  GGGPSQNWNQGYSNYWNQGYGSYGYNSQGYGGYGGYDYTGYNSYYGYGDYSNQQSGYGKVSRRGGHQNSY
  KPY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024404
• ORF Size: 1059 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024404.2, NP_077380.2
• RefSeq Size: 1718 bp
• RefSeq ORF: 1062 bp
• Locus ID: 79256
• UniProt ID: Q9JJ54
• Cytogenetics: 14p22
• MW: 38.2 kDa
• Gene Summary: mouse homolog is an RNA binding protein that binds A+U-rich elements [RGD, Feb 2006]