Sh2b1 (NM_001048180) Rat Tagged ORF Clone

CAT#: RR200245

Sh2b1 (Myc-DDK-tagged ORF) - Rat SH2-B PH domain containing signaling mediator 1 (Sh2bpsm1), transcript variant 2, (10 ug)

Product Images

Circular map for RR200245

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Sh2b1
• Synonyms: Sh2-b; Sh2b; Sh2bpsm1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200245 representing NM_001048180
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGGTGCCCCTTCCCCAGAGGATGGGGTTTTCCCTTCTCCACCAGCGCTGCCACCACCCCCTCCCC
  CAAGTTGGCAAGAGTTCTGTGAGTCCCATGCGAGGGCTGCTGCCCTGGATCTTGCTCGCCGTTTTCGCCT
  CTATCTGGCCTCCCACCCACAATATGCAGAGCCGGGAGCAGAGGCTGCCTTTTCTGGCCGTTTTGCTGAG
  CTCTTCCTGCAGCACTTCGAAGCTGAGGTGGCTCGGGCCTCGGGCTCACTCTCCCCACCTGTCTTGGCTC
  CATTGAGCCCTGGTGTGGAAATCCCACCATCACATGACCTGTCCCTTGAGAGCTGCAGGGTGGGTGGGCC
  CCTGGCAGTGTTGGGCCCTTCTCGATCTTCTGAGGACCTGGCTGGCCCCCTTCCTTCCTCAGTCTCTTCC
  TCTACAACGTCCTCAAAGCCGAAGCTCAAGAAACGCTTCTCCCTCCGCTCGGTGGGTCGTTCAGTCAGAG
  GTTCTGTCCGAGGCATCCTGCAGTGGCGGGGGGCTGTTGAATCTCCCTCCCAAGCTGGGCCTCTGGAGAC
  CACATCAGGTCCTCCAGTTCTAGGTGGAAACAGCAACTCCAACTCCTCTGGTGGTGCTGGGACAGTTGGT
  AGGGCATTGGCCAACGATGGCACATCCCCTGGGGAGAGATGGACTCATCGCTTTGAGAGGCTAAGGCTAA
  GTCGTGGAGGGGGAACCTTGAGAGACGGAGCAGGAGTGATACAGAGAGAAGAGCTGCTGAGTTTCATGGG
  GGCTGAAGAGGCTGCCCCTGACCCAGCAGGAGTAGGTCGTGGAGGAGGGGCAGCTGGGCTGACCTCGGGA
  GGAGGAGGGCAGCCTCAGTGGCAGAAATGTCGATTACTGCTCCGGAGTGAAGGAGAAGGAGGAGGAGGAA
  GTCGCTTGGAGTTCTTTGTACCACCCAAGGCATCCCGGCCCCGTCTTAGCATTCCCTGTTCTACTATTAC
  TGATGTCCGCACAGCCACAGCCCTGGAGATGCCTGACAGGGAGAACACGTTTGTGGTTAAGGTAGAAGGC
  CCTTCAGAGTACATCCTGGAGACAACTGATGCACTTCATGTGAAGGCCTGGGTGTCTGACATCCAAGAGT
  GCCTAAGCCCAGGACCCTGCCCTGCTATCAGCCCCCGTCCCATGACCCTTCCCCTGGCCCCTGGGACCTC
  CTTCCTCACAAAGGATAACACAGAGAGCCTGGAGTTGCCCTGCCTGAATCATTCAGAGAGTCTGCCTAGC
  CAGGATCTTCTTCTGGGACCCAGCGAGAGTAACGACCGCCTGTCGCAGGGAGCTTATGGAGGCCTCTCAG
  ACCGGCCGTCAGCGTCCTTCTCCCCTAGTTCTGCCTCCATTGCTGCTTCCCATTTTGACTCAATGGAACT
  GCTTCCTCCAGAGTTGCCCCCTCGGATTCCCATTGAGGAGGGGCCTCCAGCAGGGACAGTTCATCCCCTC
  TCTACCCCGTACCCTCCCCTGGATACTCCTGAAGCAGCCACAGGGTCATTCCTCTTTCAAGGGGAGGCAG
  AGGGGGGTGAGGGGGACCAGCCCCTCTCAGGCTACCCTTGGTTCCACGGCATGCTCTCTCGGCTCAAAGC
  TGCCCAGTTAGTGTTAGAAGGAGGTACCAGCTCCCATGGTGTCTTCTTGGTACGCCAGAGTGAGACAAGA
  CGTGGTGAATATGTCCTCACTTTCAACTTCCAGGGCAAGGCTAAGCACCTGCGTTTGTCACTAAATGAGG
  AGGGTCAGTGCCGGGTCCAACATCTGTGGTTCCAGTCCATTTTCGATATGCTTGAGCACTTCCGGGTGCA
  CCCCATCCCTCTGGAGTCTGGAGGCTCCAGTGATGTTGTCCTTGTCAGCTATGTGCCCTCCCAGCGGCAG
  CAGGGCCGGGAGCAGGCTGGGAGCCATGCAGGGGTGTGCGAGGGCGACCGATGCTACCCCGATGCCTCCT
  CCACCCTCCTGCCCTTCGGAGCGAGTGACTGTGTAACGGAGCACCTCCCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200245 representing NM_001048180
  Red=Cloning site Green=Tags(s)
  
  MNGAPSPEDGVFPSPPALPPPPPPSWQEFCESHARAAALDLARRFRLYLASHPQYAEPGAEAAFSGRFAE
  LFLQHFEAEVARASGSLSPPVLAPLSPGVEIPPSHDLSLESCRVGGPLAVLGPSRSSEDLAGPLPSSVSS
  STTSSKPKLKKRFSLRSVGRSVRGSVRGILQWRGAVESPSQAGPLETTSGPPVLGGNSNSNSSGGAGTVG
  RALANDGTSPGERWTHRFERLRLSRGGGTLRDGAGVIQREELLSFMGAEEAAPDPAGVGRGGGAAGLTSG
  GGGQPQWQKCRLLLRSEGEGGGGSRLEFFVPPKASRPRLSIPCSTITDVRTATALEMPDRENTFVVKVEG
  PSEYILETTDALHVKAWVSDIQECLSPGPCPAISPRPMTLPLAPGTSFLTKDNTESLELPCLNHSESLPS
  QDLLLGPSESNDRLSQGAYGGLSDRPSASFSPSSASIAASHFDSMELLPPELPPRIPIEEGPPAGTVHPL
  STPYPPLDTPEAATGSFLFQGEAEGGEGDQPLSGYPWFHGMLSRLKAAQLVLEGGTSSHGVFLVRQSETR
  RGEYVLTFNFQGKAKHLRLSLNEEGQCRVQHLWFQSIFDMLEHFRVHPIPLESGGSSDVVLVSYVPSQRQ
  QGREQAGSHAGVCEGDRCYPDASSTLLPFGASDCVTEHLP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001048180
• ORF Size: 2010 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001048180.1, NP_001041645.1
• RefSeq Size: 2791 bp
• RefSeq ORF: 2013 bp
• Locus ID: 89817
• UniProt ID: Q62985
• Cytogenetics: 1q36
• MW: 70.9 kDa
• Gene Summary: interacts with kinase-active tyrosyl-phosphorylated JAK2 [RGD, Feb 2006]