Klf4 (NM_053713) Rat Untagged Clone

CAT#: RN214949

Klf4 (untagged ORF) - Rat Kruppel-like factor 4 (gut) (Klf4), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Klf4
• Synonyms: GKLF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN214949 representing NM_053713
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGCAGCCACCTGGCGAGTCTGACATGGCTGTCAGCGACGCTCTGCTCCCGTCCTTCTCCACGTTCG
  CGTCCGGCCCGGCGGGAAGGGAGAAGACACTGCGTCCAGCAGGTGCCCCGACTAACCGTTGGCGAGAGGA
  ACTCTCTCACATGAAGCGACTTCCCCCACTTCCCGGCCGCCCCTACGACCTGGCGGCGACGGTGGCCACA
  GACCTGGAAAGTGGTGGAGCTGGTGCAGCTTGCAGCAGTAACAACCCGGCCCTACCCCGGAGGGAGACCG
  AGGAGTTCAACGATCTCCTGGACCTAGACTTTATCCTTTCCAACTCGCTATCCCACCAGGAATCGGTGGC
  CGCCACCGTGACCACCTCGGCGTCAGCTTCATCCTCGTCTTCCCCAGCTAGCAGCGGCCCTGCCAGCGCG
  CCCTCCACCTGCAGCTTCAGCTATCCGATCCGGGCCGGGGGTGACCCGGGCGTGGCTGCGGGCAACACAG
  GTGGAGGGCTCCTCTACAGCCGAGAATCTGCGCCACCTCCCACGGCCCCCTTCAACCTGGCGGACATCAA
  TGACGTGAGCCCCTCGGGCGGCTTCGTGGCTGAGCTCCTGCGGCCGGAGTTGGACCCAGTATACATTCCG
  CCACAGCAGCCTCAGCCGCCAGGTGGCGGGCTGATGGGCAAGTTTGTGCTGAAGGCGTCTCTGAGCACCC
  CTGGCAGCGAGTACACCAGCCCTTCGGTCATCAGTGTTAGCAAAGGAAGCCCAGACGGCAGCCACCCTGT
  GGTAGTGGCGCCCTACAGCGGTGGCCCGCCGCGTATGTGCCCCAAGATTAAGCAAGAGGCCGTCCCGTCC
  TGCACGGTCAGCCGGTCCCTAGAGGCCCACTTGAGCGCTGGACCCCAGCTCAGCAACGGCCACAGGCCCA
  ACACACACGACTTCCCCCTGGGGCGGCAGCTCCCCACCAGGACTACCCCTACACTGAGTCCCGAGGAACT
  GCTGAACAGCAGGGACTGTCACCCTGGCCTGCCTCTTCCCCCAGGATTCCATCCCCATCCGGGGCCCAGC
  TACCCTCCTTTCCTGCCAGACCAGATGCAGTCGCAAGTCCCCTCTCTCCATTATCAAGAGCTCATGCCAC
  CGGGATCCTGCCTGCCAGAGGAGCCCAAGCCAAAGAGGGGAAGAAGGTCTTGGCCCCGGAAAAGAACAGC
  CACCCACACTTGTGACTATGCAGGCTGTGGCAAAACCTATACGAAGAGTTCTCATCTCAAGGCACACCTG
  CGAACTCACACAGGCGAGAAACCTTACCACTGTGACTGGGACGGCTGTGGGTGGAAATTCGCCCGCTCAG
  ATGAACTGACCAGGCACTACCGCAAACACACCGGGCACCGGCCCTTTCAGTGCCAGAAGTGCGACAGGGC
  CTTTTCCAGGTCGGACCACCTTGCCTTACACATGAAGAGGCACTTTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_053713
• Insert Size: 1449 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053713.1, NP_446165.1
• RefSeq Size: 2393 bp
• RefSeq ORF: 1449 bp
• Locus ID: 114505
• Cytogenetics: 5q24
• Gene Summary: a transcription factor that works with Sp1 to activate the Laminin gamma1 chain gene [RGD, Feb 2006]