Polr3c (NM_001012081) Rat Untagged Clone

CAT#: RN214037

Polr3c (untagged ORF) - Rat polymerase (RNA) III (DNA directed) polypeptide C (62kD) (Polr3c), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Polr3c
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN214037 representing NM_001012081
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTCAAGCAGAAATTAAACTGTGCTCTTTGTTGCTACAAGAGCATTTTGGTGAGATTGTAGAAAAAA
  TTGGAGTCCACCTAATCAGAACTGGGAGCCAGCCACTTAGAGTAATTGCCCATGACACAAAAGCATCACT
  GGACCAGGTGAAAAAAGCCCTTTGTGTCCTCATTCACCATAACCTGGTCATCTATCATGTGCATAAACGC
  GGTGTGGTGGAGTATGAAGCCCAATGCAGCCGGGTACTGCGGATGCTTCGGTACCCCCGGTACATCTATA
  CTACCAAAACACTGTATGGTGACACTGGAGAGCTGATTGTTGAGGAGCTTCTGCTGAATGGCAAAATGAC
  AATGTCGGCTGTTGTGAAGAAAGTAGCAGACCGACTCACAGAGACCATGGAGGATGGCAAGACCATGGAC
  TACGCTGAGGTGTCAAATGCATTTGTTCGACTGGCAGATACTCACTTTGTACAGCGCTGTCCCCTGGTTC
  CTGACACTGACAGTTCTGACCCCGGGCCGCCGCCACCTGCCCCAAACCTTGTCATTAATGAAAAGGACAT
  GTACCTAGTTCCCAAACTGAGCTTGATAGGAAAAGGTAAGAGGAGGAGATCATCTGACGAAGATGCTGCT
  GGAGAGCCCAAGGCCAAGAAACCAAGATGCACAGATAATGAGGAGCCCACACCAGACGATGGGATTTATT
  GGCAAGTCAACCTGGACAGATTCCACCAGCACTTCCGTGACCAAGCAATTGTGAGCGCAGTGGCAAACCG
  AATGGACCAGACAAGCAGCGAGATTGTGCGGACAATGCTCCGAATGAGTGAGATTACCACTCCCTCTGGT
  GCGCCGTTTACTCAGCCACTGTCCTCCAATGAGATCTTCAGATCCCTGCCCGTCGGATATAATATCTCTA
  AACAGGTTCTGGATCAGTACCTCACGCTGCTGGCAGATGACCCACTAGAATTTATTGGAAAGGCTGGCGA
  CAGTGGCGGAGGAATGTATGTCATCAACCTCCATAAGGCATTAGCATCCCTATCCACTGCAACTCTGGAG
  TCTGTCATCCAAGAGAGATTCGGGTCTCGCTGTGCCCGGATATTCCGTCTGGTGTTGCAGAAGAAGCACC
  TGGAGCAGAAGCAGGTGGAGGACTTTGCAATGATCCCTGCAAAGGAGGCAAAGGACATGCTGTATAAGAT
  GCTCTCGGAGAACTTCATATCACTGCAGGAAATTCCTAAAACGCCAGACCACGCCCCGTCCAGGACCTTC
  TATCTGTACACCGTGAATGTGCTGTCAGCCGCCAGGATGCTGTTGCACAGGTGCTACAAGAGCATAGCCA
  ACTTGATAGAAAGGCGGCAATTTGAAACAAAAGAGAACAAGCGGCTACTAGAAAAGTCTCAGCGGGTGGA
  AGCCATCATGGCATCAATGCAGGCCACGGGTGCAGAGGAGGTACAGCTGCAGGAGATCGAGGAGATGATC
  ACAGCCCCCGAGCGGCAGCAGCTGGAGACACTGAAACGCAACGTTAACAAGTTGGATGCCAGTGAGATCC
  AGGTAGATGAAACCATCTTCTTACTGGAATCATACATCGAGAGCACCATGAAGAGACAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001012081
• Insert Size: 1602 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012081.1, NP_001012081.1
• RefSeq Size: 2394 bp
• RefSeq ORF: 1602 bp
• Locus ID: 310685
• UniProt ID: Q5XIL3
• Cytogenetics: 2q34
• Gene Summary: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct with other members of the subcomplex RNA Pol III binding to the TFIIIB-DNA complex via the interactions between TFIIIB and POLR3F. May be involved either in the recruitment and stabilization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts induce type I interferon and NF-Kappa-B through the RIG-I pathway. Preferentially binds single-stranded DNA (ssDNA) in a sequence-independent manner.[UniProtKB/Swiss-Prot Function]