Appl2 (NM_001108741) Rat Untagged Clone

CAT#: RN213887

Appl2 (untagged ORF) - Rat adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 2 (Appl2), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Appl2
• Synonyms: RGD1563028
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN213887 representing NM_001108741
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCGCCGTGGACAAGCTCCTGCTGGAGGAGGCGCTGCAGGACAGCCCCCAGACGAGGTCCCTGCTGA
  GTGTCTTTGAAGAGGATGCTGGCACCCTCACAGACTACACCAACCAGCTGCTGCAGGCGATGCAGCGTGT
  CTATGGTGCACAGAATGAAATGTGTCTGGCCACGCAACAGCTTTCCAGGCAGCTGCTGGCATATGAAAAA
  CAGAATTTTGCACTGGGCAAAGGCGACGAAGAAGTAATTTCTACACTACATTATTTTTCCAAAGTGATGG
  ATGAGCTCAATGGTCTCCACTCAGAGCTGGCCAAGCAACTGGCAGACACTATGGTTCTCCCTGTTATACA
  GTTCCGAGAGAAGGATCTCACAGAGGTCAGCACTTTGAAGGATCTCTTTGGACTTGCCAGCAATGAGCAT
  GACCTGTCAATGGCAAAATACAGCAGGCTCCCTAAGAGAAAGGAGAACGAGAGGGTGAAGACGGACGTTG
  CAAAGGAGGTGGCAGCTGCCCGGCGGAAACAGCATCTGTCATCCCTTCAGTACTACTGTGCACTGAACGC
  ACTGCAGTACCGCAAGAGGGCAGCCATGATGGAGCCGCTCATAGGCTTTGCCCATGGACAGATTAACTTT
  TTCAAGAAAGGAGCAGAGATGTTCTCCAAAAGTATGGACGGTTTCTTATCATCCGTTACAGACATGGTTC
  AGAGCATTCAGGTGGAACTGGAAGCCGAGGCCGACAAGATGCGGGTGTCCCAGCAGGAGCTGCTTTCAGT
  CAGTGAGTCTGTTTACACTCCGGACATCGATGTGGCCACACCGCAGATCAACAGGAACCTCATCCAGAAG
  ACTGGCTACCTCAATCTCAGAAACAAGACGGGACTGGTCACCACCACATGGGAAAGGCTGTACTTCTTCA
  CCCAAGGCGGGAACCTCATGTGCCAGCCTAGGGGAGCTGTGGCTGGAGGCTTGATTCAGGACCTGGACAA
  CTGCTCTGTGATGGCGGTGGATTGCGAAGACCGACGATACTGCTTCCAGATCTCCACGCCCAGTGGCAAA
  CCGGGGATAATTCTCCAGGCAGAGAGCAGAAAGGAATATGAAGAGTGGATATGTGCAATAAACAATATCT
  CCAGGCAGATCTACCTGACAGACAACCCAGAGGCAGTAGCCATCAAGTTGAATCAGACGGCTCTTCAGGC
  GGTGACTCCGATTACAAGCTTTGGGAAAAAACAAGAAAGCTTCTACTTCAGTCAAAACATAAAAAATTCA
  GACACAGGATATGTCAAGATTGTTCCCAAAGCAGCAGCCAGCATTCCTGAGACAGAGGAACTGATTGCAC
  CTGGGACACCCATTCAGTTTGACATTGTACTTCCTGCAACGGAATTCCTTGACCAGAACAGAGGCAGCAG
  GCGCATCAACCCTTTTGGTGAGACGGAGGATGACTCATTTCCGGACGCAGAAGACTCTCTCTTGCAGCAG
  ATGTTCATCGTTCGGTTTTTGGGATCGATGGCAGTTAAGACAGACAGCACTACGGAAGTGATTTATGAAG
  CGATGCGACAAGTACTGGCTGCTCGGGCTATTCACAACATCTTCCGCACCACGGAGTCCCACCTGATGGT
  TACCAGTCAGACTCTGAGGTTGATAGATCCTCAGACTCAAGTGTCACGGGCCTGTTTTGAGCTCACCAGT
  GTCACACAGTTTGCTGCTCATCAAGAAAACAAAAGGCTGGTTGGCTTCGTCATCCGAGTGCCGGAGTCTA
  CAGGAGAAGAGTCTCTGAGCACATACATTTTTGAAAGCAACTCAGAAGGCGAGAAGATATGTTATGCTAT
  TAATTTGGGGAAAGAAATTATTGAGGTTCAAAAGGATCCAGAAGCACTGGCTCGATTAATGCTGTCTGTA
  CCACTAACCAATGACGGAAAATATGTACTGTTAAACGATCAAGCAGATGACACTGGCGGAAGTCCAAGTG
  ACCACAGAGGCGCAGAGTCTGAAGCATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001108741
• Insert Size: 1989 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001108741.1, NP_001102211.1
• RefSeq Size: 2964 bp
• RefSeq ORF: 1989 bp
• Locus ID: 362860
• UniProt ID: B4F779
• Cytogenetics: 7q13
• Gene Summary: Multifunctional adapter protein that binds to various membrane receptors, nuclear factors and signaling proteins to regulate many processes, such as cell proliferation, immune response, endosomal trafficking and cell metabolism. Regulates signaling pathway leading to cell proliferation through interaction with RAB5A and subunits of the NuRD/MeCP1 complex (By similarity). Plays a role in immune response by modulating phagocytosis, inflammatory and innate immune responses. In macrophages, enhances Fc-gamma receptor-mediated phagocytosis through interaction with RAB31 leading to activation of PI3K/Akt signaling. In response to LPS, modulates inflammatory responses by playing a key role on the regulation of TLR4 signaling and in the nuclear translocation of RELA/NF-kappa-B p65 and the secretion of pro- and anti-inflammatory cytokines. Also functions as a negative regulator of innate immune response via inhibition of AKT1 signaling pathway by forming a complex with APPL1 and PIK3R1 (By similarity). Plays a role in endosomal trafficking of TGFBR1 from the endosomes to the nucleus (By similarity). Plays a role in cell metabolism by regulating adiponecting ans insulin signaling pathways and adaptative thermogenesis (By similarity). In muscle, negatively regulates adiponectin-simulated glucose uptake and fatty acid oyidation by inhibiting adiponectin signaling pathway through APPL1 sequestration thereby antagonizing APPL1 action (By similarity). In muscles, negativeliy regulates insulin-induced plasma membrane recruitment of GLUT4 and glucose uptake through interaction with TBC1D1 (By similarity). Plays a role in cold and diet-induced adaptive thermogenesis by activating ventromedial hypothalamus (VMH) neurons throught AMPK inhibition which enhances sympathetic outflow to subcutaneous white adipose tissue (sWAT), sWAT beiging and cold tolerance (By similarity). Also plays a role in other signaling pathways namely Wnt/beta-catenin, HGF and glucocorticoid receptor signaling (By similarity). Positive regulator of beta-catenin/TCF-dependent transcription through direct interaction with RUVBL2/reptin resulting in the relief of RUVBL2-mediated repression of beta-catenin/TCF target genes by modulating the interactions within the beta-catenin-reptin-HDAC complex (By similarity). May affect adult neurogenesis in hippocampus and olfactory system via regulating the sensitivity of glucocorticoid receptor. Required for fibroblast migration through HGF cell signaling (By similarity).[UniProtKB/Swiss-Prot Function]