Hsd17b11 (NM_001004209) Rat Untagged Clone

CAT#: RN213495

Hsd17b11 (untagged ORF) - Rat hydroxysteroid (17-beta) dehydrogenase 11 (Hsd17b11), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Hsd17b11
• Synonyms: Dhrs8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN213495 representing NM_001004209
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGTACCTTCTTGACCTGATCCTGCTGCTGCCTCTGCTTATTGTCTTCTGCATTGAGTCTTTCATCA
  AGCGTTTGATTCCCAAGAAGAAAAAATCCGTCGCTGGAGAGATCGTTCTGATCACCGGAGCTGGTCATGG
  GATTGGAAGACTGACCGCCTACGAATTTGCCAAACTAAACACCAAACTGGTTCTGTGGGATATCAATAAG
  AATGGCATCGAGGAAACAGCGGCGAAGTGCAGGAAGCTAGGTGCCCAGGTCCATCCCTTTGTGGTGGACT
  GCAGCCAGCGGGAAGAGATTTACAGCGCTGTGAGAAAGGTAAAGGAAGAAGTTGGAGATGTTAGCATTCT
  GGTAAATAATGCCGGCGTTGTCTACACTGCAGATCTATTTGCTACGCAGGACCCTCAGATTGAAAAGACT
  TTCGAAGTCAATGTGCTTGCACACTTTTGGACCACCAAGGCATTTCTCCCAGCCATGATGAAGAATAATC
  ATGGTCATGTTGTCACCGTGGCGTCGGCAGCAGGGCACACCGTCGTTCCCTTCCTGCTGGCTTACTGTTC
  TAGCAAGTTTGCTGCTGTTGGCTTCCACAGAGCCTTGACTGATGAACTGGCTGCCTTGGGATGCACTGGA
  GTCCGAACATCCTGCCTCTGCCCTAACTTCATAAATACCGGCTTCATCAAGAACCCGAGCACCAATTTAG
  GGCCCACCCTGGAACCTGAGGAGGTGGTAGAGCATCTCATGCACGGGATCCTCACTAACCAGAAGATGAT
  CTTCGTCCCGGGCTCTATAGCGCTTTTGACAGTATTGGAAAGGGTGTTTCCTGAGAGATTCCTGGACGTT
  CTAAAGCGCAGGATCAACGTTAAGTTCGATGCTGTTGTTGGCTACAAAGATAAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001004209
• Insert Size: 897 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001004209.1, NP_001004209.1
• RefSeq Size: 1622 bp
• RefSeq ORF: 897 bp
• Locus ID: 289456
• UniProt ID: Q6AYS8
• Cytogenetics: 14p22
• Gene Summary: Can convert androstan-3-alpha,17-beta-diol (3-alpha-diol) to androsterone in vitro, suggesting that it may participate in androgen metabolism during steroidogenesis. May act by metabolizing compounds that stimulate steroid synthesis and/or by generating metabolites that inhibit it. Has no activity toward DHEA (dehydroepiandrosterone), or A-dione (4-androste-3,17-dione), and only a slight activity toward testosterone to A-dione.[UniProtKB/Swiss-Prot Function]