Gpr89b (NM_001139486) Rat Untagged Clone

CAT#: RN213252

Gpr89b (untagged ORF) - Rat G protein-coupled receptor 89 (Gpr89), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Gpr89b
• Synonyms: Gpr89; RGD1304837
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN213252 representing NM_001139486
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTTCTTGATCGACTCCAGCATCATGATCACCTCCCAGATACTGTTCTTTGGATTCGGGTGGCTTT
  TCTTCATGCGCCAGTTGTTTAAGGACTATGAGGTTCGTCAGTATGTGGTGCAGGTGATCTTCTCCGTGAC
  CTTTGCGTTTTCCTGCACCATGTTCGAGCTTATCATCTTTGAAATCTTGGGAGTTCTGAACAGCAGCTCC
  CGTTACTTTCACTGGAAAATGAACCTGTGTGTCATTCTGCTCATCCTGGTTTTCATGGTTCCCTTCTACA
  TTGGTTATTTTATCGTGAGTAACATCCAACTGTTGCACAAACAGCGTCTGCTCTTTTCCTGTCTCTTATG
  GCTGACCTTTATGTACTTCTTCTGGAAGCTGGGAGATCCATTCCCCATACTCAGCCCAAAACATGGGATC
  TTGTCCATAGAGCAGCTCATCAGCCGGGTAGGTGTGATTGGGGTGACACTCATGGCTCTGCTTTCCGGAT
  TCGGTGCTGTCAACTGCCCCTACACATACATGTCCTACTTCCTCAGGAATGTGACTGACACGGATATCCT
  TGCCCTGGAACGGCGACTGCTGCAGACCATGGACATGATCATAAGCAAAAAGAAAAGGATGGCGGTGACC
  CGGAGAACAATGTTCCAGAGGGGGGATGTGCAGAACAAGCCATCGGGACTCTGGGGCATGCTGAAGAGTG
  TCACTGCCTCGGCCCCAGGAAGTGAAAATCTGACTCTTATCCAACAGGAAGTAGATGCTTTAGAAGAACT
  GAGCAGGCAGCTTTTCCTGGAAACAGCCGATCTGTATGCTACAAAGGAAAGAATTGAATACTCCAAAACA
  TTCAAGGGGAAATATTTTAATTTCCTGGGTTATTTTTTCTCTATTTATTGTGTCTGGAAAATTTTTATGG
  CAACCATCAATATTGTTCTTGATCGGGTTGGGAAAACTGACCCTGTCACCAGAGGCATCGAGATCACTGT
  GAATTATCTGGGAATCCAGTTCGATGTGAAGTTCTGGTCTCAGCACATTTCTTTCATCCTGGTTGGAATA
  ATCATTGTCACGTCCATCAGGGGACTGCTGATCACACTTACCAAGTTCTTTTACGCCATCTCCAGCAGTA
  AGTCCTCCAATGTCATCGTCCTGCTCTTAGCACAGATAATGGGAATGTACTTTGTGTCCTCTGTACTGCT
  GATCCGAATGAGTATGCCCCCAGAATACCGCACCATAATCACAGAGGTCCTTGGAGAGCTTCAGTTCAAC
  TTCTATCACCGCTGGTTTGACGTCATCTTCCTGGTCAGTGCCCTCTCCAGCATATTGTTCCTCTATCTGG
  CTCACAAGCAGGCGCCAGAGAAGCATATGGCCCCTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001139486
• Insert Size: 1368 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001139486.1, NP_001132958.1
• RefSeq Size: 1824 bp
• RefSeq ORF: 1368 bp
• Locus ID: 362003
• Cytogenetics: 2q34