Eif2b1 (NM_172029) Rat Untagged Clone

CAT#: RN211873

Eif2b1 (untagged ORF) - Rat eukaryotic translation initiation factor 2B, subunit 1 alpha (Eif2b1), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Eif2b1
• Synonyms: eIF-2a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN211873 representing NM_172029
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGACGGTGAGTTAATCAAGTACTTTAAGTCCCAGATGAAAGGAGACCCTAACATGGCCTCGGCGG
  TGGCTGCCATCCAGACCTTGCTGGAATTCTTGAAGAGAGATAAAGGAGAGACAATCCAGGGCCTGAGAGC
  CCATCTCACCAAAGCCATCGAAACCCTCTGCGCTGTGGACTCCTCGGTGGCCGTGTCCTCGGGCGGGGAA
  CTCTTCCTTCGCTTCATCAGCCTCACCTCCCTGGAGTACTCCGACTACTCCAAATGTAAAAAGATCATGA
  TCGAGAGAGGAGAGCTTTTCCTGAGCAGAATATCCCTGTCAAGAACTAAGATCGCCAGTCTATGCCATGC
  TTTTATCAAAGACGGGGCGAGAATCTTGACTCACGCCTACTCCAGAGTTGTCCTGAGGGTCCTGGAAGAA
  GCCGTGGCAGCCAAGAAACGTTTCAGTGTGTATATCACGGAGTCACAGCCTGATTTATCTGGTAAGAAAA
  TGGCCAAAGCCCTCTGCCACCTCAATGTCCCTGTCACTGTGGTGCTGGACGCTGCTGTTGGCTATATCAT
  GGAGAAAGTAGACCTTGTCATCGTTGGTGCTGAAGGAGTGGTAGAGAATGGAGGAATTATTAACAAGATC
  GGAACCAACCAGATGGCCGTGTGTGCCAAAGCCCAGAACAAGCCCTTCTATGTGGTTGCAGAAAGTTTCA
  AGTTTGTACGGCTCTTTCCACTCAACCAGCAAGATGTCCCAGATAAGTTTAAGTACAAGGCAGACACCCT
  GAAGTCTGTGCAGGCTGGACAGGACCTCAAAGAGGAACACCCATGGGTCGACTACACCTCCCCATCCCTC
  ATCACACTGCTGTTTACGGACTTAGGTGTGTTGACACCATCCGCTGTAAGCGACGAGCTCATCAAGCTGT
  ACCTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_172029
• Insert Size: 918 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172029.2, NP_742026.1
• RefSeq Size: 1502 bp
• RefSeq ORF: 918 bp
• Locus ID: 64514
• UniProt ID: Q64270
• Cytogenetics: 12q15
• Gene Summary: alpha subunit of eukaryotic peptide initiation factor 2B involved with mRNA translation into protein; may regulate protein synthesis when peptide chain initiation is inhibited [RGD, Feb 2006]