Gad2 (NM_012563) Rat Untagged Clone

CAT#: RN211126

Gad2 (untagged ORF) - Rat glutamate decarboxylase 2 (Gad2), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Gad2
• Synonyms: gad65
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN211126 representing NM_012563
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCATCTCCGGGCTCTGGCTTTTGGTCCTTCGGATCTGAAGATGGCTCTGGGGATCCTGAGAACCCGG
  GAACAGCGAGAGCCTGGTGCCAGGTGGCCCAAAAGTTCACGGGCGGCATCGGAAACAAGCTATGCGCTCT
  GCTCTACGGAGACTCTGAGAAGCCAGCAGAGAGCGGCGGGAGCGTGACCTCGCGGGCCGCCACTCGGAAG
  GTCGCCTGCACCTGTGACCAAAAACCCTGCAGCTGCCCCAAAGGAGATGTCAATTATGCACTTCTCCACG
  CAACAGACCTGCTGCCAGCCTGTGAAGGAGAAAGGCCCACTCTCGCATTTCTGCAAGATGTAATGAACAT
  TTTGCTTCAGTACGTGGTGAAAAGTTTTGATAGATCAACTAAAGTGATTGATTTCCATTACCCCAATGAG
  CTTCTTCAAGAGTATAATTGGGAATTGGCAGACCAACCGCAAAATCTGGAGGAAATTTTGACGCACTGCC
  AAACAACTCTAAAATATGCGATTAAAACAGGGCATCCCCGATATTTTAATCAGCTGTCTACCGGATTGGA
  TATGGTTGGATTAGCAGCAGATTGGTTGACATCAACAGCAAACACGAACATGTTTACCTATGAGATCGCC
  CCTGTATTTGTACTACTGGAATATGTGACACTAAAGAAAATGAGGGAAATCATTGGCTGGCCAGGAGGCT
  CTGGCGATGGAATCTTTTCTCCTGGTGGTGCCATCTCCAACATGTACGCCATGCTCATTGCCCGCTATAA
  GATGTTTCCAGAAGTCAAGGAAAAGGGGATGGCGGCGGTGCCCAGGCTCATCGCATTCACGTCAGAGCAT
  AGTCACTTTTCTCTCAAGAAGGGAGCTGCAGCCTTGGGGATCGGAACAGACAGCGTGATTCTGATTAAAT
  GTGATGAGAGAGGGAAAATGATCCCATCTGACCTTGAAAGAAGAATCCTTGAAGTCAAACAGAAAGGATT
  TGTTCCTTTCCTGGTGAGTGCCACAGCTGGAACCACTGTGTACGGGGCTTTTGATCCTCTCTTGGCTGTA
  GCTGACATCTGCAAAAAATATAAGATCTGGATGCATGTGGATGCTGCTTGGGGTGGAGGGTTACTGATGT
  CTCGGAAACACAAGTGGAAGCTGAACGGTGTGGAGAGGGCCAACTCTGTGACATGGAATCCCCACAAGAT
  GATGGGTGTCCCCTTGCAATGTTCGGCTCTCCTGGTCAGAGAGGAGGGACTGATGCAGAGCTGCAACCAG
  ATGCATGCTTCCTACCTCTTTCAGCAAGATAAGCACTATGACCTGTCCTATGACACGGGAGACAAGGCCT
  TGCAGTGTGGACGCCACGTCGATGTCTTTAAATTATGGCTCATGTGGAGAGCAAAGGGGACTACTGGATT
  TGAAGCTCACATTGATAAGTGTTTGGAGCTGGCAGAGTATTTATACAATATCATTAAAAACCGAGAAGGA
  TATGAAATGGTGTTCGATGGGAAGCCTCAGCACACAAATGTCTGCTTCTGGTTTGTACCTCCTAGTTTGC
  GAGTTCTGGAAGACAATGAAGAGAGAATGAGCCGCCTCTCAAAGGTGGCGCCAGTGATTAAAGCCAGAAT
  GATGGAGTATGGGACCACAATGGTCAGCTACCAACCCTTAGGAGATAAGGTCAACTTCTTCCGCATGGTC
  ATCTCAAACCCTGCAGCAACTCACCAAGACATTGACTTCCTCATTGAAGAAATCGAACGCCTGGGACAAG
  ATTTGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_012563
• Insert Size: 1758 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012563.1, NP_036695.1
• RefSeq Size: 1966 bp
• RefSeq ORF: 1758 bp
• Locus ID: 24380
• UniProt ID: Q05683
• Cytogenetics: 17q12.3
• Gene Summary: catalyzes synthesis of gamma-Aminobutyric acid (GABA), the most widely distributed inhibitory neurotransmitter [RGD, Feb 2006]