Sf3a2 (NM_001011986) Rat Untagged Clone

CAT#: RN208102

Sf3a2 (untagged ORF) - Rat splicing factor 3a, subunit 2 (Sf3a2), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Sf3a2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN208102 representing NM_001011986
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTTTCAGCACCGACCTGGAGGCAAAACTGGGAGTGGGGGCGTGGCCTCCTCCTCAGAGAGCAACC
  GGGATCGAAGAGAGCGCCTGCGGCAACTGGCCCTGGAAACCATCGACATCAATAAGGACCCGTATTTCAT
  GAAGAACCACCTGGGATCCTATGAGTGCAAACTCTGCTTGACTCTGCATAACAATGAGGGGAGCTACCTG
  GCCCATACCCAAGGGAAGAAACATCAGACTAACTTGGCCCGGCGAGCTGCCAAGGAGGCCAAAGAAGCCC
  CCGCCCAGCCTGCACCAGAGAAGGTCAAGGTGGAGGTGAAGAAGTTTGTGAAGATTGGCCGCCCTGGCTA
  CAAAGTGACCAAGCAGAGGGACACAGAGATGGGCCAGCAGAGCCTACTGTTCCAGATTGACTACCCTGAG
  ATTGCAGAGGGTGTCATGCCCCGCCACCGCTTCATGTCTGCCTATGAGCAGAGGATCGAGCCCCCGGACC
  GCCGCTGGCAGTACCTCCTCATGGCTGCTGAGCCCTATGAGACCATCGCCTTCAAGGTGCCGAGCCGCGA
  GATCGACAAGGCTGAGGGCAAGTTTTGGACCCACTGGAACCGAGAGACCAAGCAGTTTTTTCTTCAGTTC
  CACTTTAAGATGGAGAAGCCCCCAGCCCCTCCCAGCCTCCCAGCAGGACCCCCAGGCGTCAAGCGGCCCC
  CGCCCCCACTCATGAACGGACTACCTCCTCGCCCACCACTCCCTGATGCCTTGCCCCCACCTCCACCTGG
  TGGCCTGCCTCTTCCTCCTATGCCCCCCACTGGGCCTGCTCCCTCTGGGCCCCCTGGACCTCCCCAGATG
  CCCCCACCAGCTCCTGGGGTACACCCCCCAGCCCCAGTAGTACACCCACCAACATCTGGGGTCCATCCCC
  CAGCTCCTGGGGTGCACCCCCCAGCCCCAGTGGTTCATCCACCAACATCTGGGGTCCATCCCCCAGCTCC
  TGGGGTCCATCCTCCAGCCCCTGGAGTGCACCCTCCAGCCCCTGGAGTGCACCCACCAGCCCCTGGGGTG
  CACCCCCCAGCTCCAGGGGTCCACCCCCCAGCTCCAGGGGTCCACCCCCCAGCTCCAGGGGTCCACCCCC
  CAGCTCCAGGGGTCCACCCTCCTCCATCAGCTGGAGTTCATCCTCAGGCTCCAGGGGTGCATCCACCTGC
  TCCTGCAGTCCACCCTCAAGCCCCTGGTGTACACCCCCCAGCTCCTGGGATCCACCCTCAGGCACCAGGG
  GTGCACCCCCAGCCTCCTCCTGGAGTCCACCCTGCTGCCCCAGGGGTCCATCCTCAGCCTCCAGGGGTTC
  ACCCTACTCCTATGCCCCCCATGCTAAGGCCCCCCCTTCCCTCGGATGGCCCTGGGAACATGCCTCCGCC
  TCCCCCAGGCAACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001011986
• Insert Size: 1416 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001011986.1, NP_001011986.1
• RefSeq Size: 1633 bp
• RefSeq ORF: 1416 bp
• Locus ID: 299620
• UniProt ID: Q6AXT8
• Cytogenetics: 7q11
• Gene Summary: Involved in pre-mRNA splicing as a component of the splicing factor SF3A complex that contributes to the assembly of the 17S U2 snRNP, and the subsequent assembly of the pre-spliceosome 'E' complex and the pre-catalytic spliceosome 'A' complex. Involved in pre-mRNA splicing as a component of pre-catalytic spliceosome 'B' complexes, including the Bact complex. Interacts directly with the duplex formed by U2 snRNA and the intron.[UniProtKB/Swiss-Prot Function]