Ldha (NM_017025) Rat Untagged Clone

CAT#: RN207357

Ldha (untagged ORF) - Rat lactate dehydrogenase A (Ldha), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Ldha
• Synonyms: CDK1; Ldh1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RN207357 representing NM_017025.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCAGCCCTCAAGGACCAGCTGATTGTGAATCTTCTTAAGGAAGAACAGGTCCCCCAGAACAAGATT
  ACAGTTGTTGGGGTTGGTGCTGTTGGCATGGCTTGTGCCATCAGTATCTTAATGAAGGACTTGGCTGAT
  GAGCTTGCCCTTGTTGATGTCATAGAAGATAAGCTAAAGGGAGAGATGATGGATCTTCAGCATGGCAGC
  CTTTTCCTTAAGACACCAAAAATTGTCTCCAGCAAAGATTATAGTGTGACTGCAAACTCCAAGCTGGTC
  ATTATCACCGCGGGGGCCCGTCAGCAAGAGGGAGAGAGCCGGCTCAATTTGGTCCAGCGAAACGTGAAC
  ATCTTCAAGTTCATCATTCCAAATGTTGTGAAATACAGTCCACAGTGCAAACTGCTCATCGTCTCAAAC
  CCAGTGGATATCTTGACCTACGTGGCTTGGAAGATCAGCGGCTTCCCCAAAAACAGAGTTATTGGAAGT
  GGTTGCAATCTGGATTCGGCTCGGTTCCGTTACCTGATGGGAGAAAGGCTGGGAGTTCATCCACTGAGC
  TGTCACGGGTGGGTCCTGGGAGAGCATGGCGACTCCAGTGTGCCTGTGTGGAGTGGTGTGAATGTCGCC
  GGCGTCTCCCTGAAGTCTCTGAACCCGCAGCTGGGCACGGATGCAGACAAGGAGCAGTGGAAGGATGTG
  CACAAGCAGGTGGTTGACAGTGCATACGAAGTGATCAAGCTGAAAGGTTACACATCCTGGGCCATTGGC
  CTCTCCGTGGCAGACTTGGCCGAGAGCATAATGAAGAACCTTAGGCGGGTGCATCCCATTTCCACCATG
  ATTAAGGGTCTCTATGGAATCAAGGAGGATGTCTTCCTCAGCGTCCCATGTATCCTGGGACAAAATGGA
  ATCTCAGATGTTGTGAAGGTGACACTGACTCCTGACGAGGAGGCCCGCCTGAAGAAGAGTGCAGATACC
  CTCTGGGGAATCCAGAAGGAGCTGCAGTTCTAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
  Protein Sequence

  MAALKDQLIVNLLKEEQVPQNKITVVGVGAVGMACAISILMKDLADELALVDVIEDKLKGEMMDLQHGS
  LFLKTPKIVSSKDYSVTANSKLVIITAGARQQEGESRLNLVQRNVNIFKFIIPNVVKYSPQCKLLIVSN
  PVDILTYVAWKISGFPKNRVIGSGCNLDSARFRYLMGERLGVHPLSCHGWVLGEHGDSSVPVWSGVNVA
  GVSLKSLNPQLGTDADKEQWKDVHKQVVDSAYEVIKLKGYTSWAIGLSVADLAESIMKNLRRVHPISTM
  IKGLYGIKEDVFLSVPCILGQNGISDVVKVTLTPDEEARLKKSADTLWGIQKELQF*
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_017025
• Insert Size: 999 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017025.2
• RefSeq Size: 1598 bp
• RefSeq ORF: 999 bp
• Locus ID: 24533
• UniProt ID: P04642
• Cytogenetics: 1q22
• MW: 36.5 kDa
• Gene Summary: mRNA expression in fibroblasts increases in response to induction by epidermal growth factor or serum [RGD, Feb 2006]