Trpm2 (NM_001011559) Rat Untagged Clone

CAT#: RN206806

Trpm2 (untagged ORF) - Rat transient receptor potential cation channel, subfamily M, member 2 (Trpm2), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Trpm2
• Synonyms: Trpm2-predicted
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN206806 representing NM_001011559
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCCTTGGACCAGAGAAGAACTGACTCTGATCAAGAGGAGGGCTTTGGGGTGCAGTCCCGGAGGG
  CCACTGATCTGGGCATGGTCCCCAATCTCCGACGAAGCAATAGCAGTCTTTGCAAGAGCAGGAGGCTTCT
  GTGCTCCTTCAGCAGTGAGAAGCAAGAAAACCTTAGCTCATGGATTCCCGAGAACATCAAGAAGAAGGAA
  TGTGTGTATTTCGTGGAAAGTTCCAAGCTCTCGGATGCAGGGAAGGTAGTGTGTGAGTGTGGTTACACCC
  ACGAGCAGCACATTGAAGTGGCCATCAAGCCTCACACCTTCCAGGGCAAGGAGTGGGACCCAAAGAAACA
  CGTCCATGAGATGCCTACAGATGCCTTTGGTGACATTGTCTTCACCGGCCTGAGCCAGAAAGTGGGGAAG
  TATGTCCGACTCTCCCAGGACACGTCGTCCATTGTCATCTACCAGCTTATGACACAGCACTGGGGCCTGG
  ATGTCCCCAGCCTCCTCATCTCTGTGACCGGTGGGGCCAAGAACTTCAACATGAAGCTGAGGTTGAAGAG
  CATCTTCCGGAGAGGCCTGGTTAAGGTGGCCCAAACCACGGGGGCCTGGATCATCACTGGGGGTTCCCAC
  ACCGGTGTGATGAAGCAGGTGGGCGAGGCGGTACGGGACTTCAGCCTAAGCAGCAGCTGCAAAGAAGGCG
  ACGTCATCACCATCGGCATAGCCACGTGGGGCACCATCCACAACCGTGAGGCACTGATCCATCCCATGGG
  AGGCTTCCCCGCTGAGTACATGCTGGATGAGGAAGGCCAAGGGAACCTGACCTGCCTGGACAGCAACCAC
  TCCCACTTCATCCTGGTGGATGATGGGACCCACGGGCAGTATGGTGTGGAGATTCCGCTGAGGACTAAGC
  TGGAGAAGTTCATATCGGAGCAAACGAAGGAAAGAGGGGGTGTGGCCATTAAGATCCCCATTGTCTGCGT
  GGTGTTGGAGGGTGGCCCTGGCACTCTGCATACCATCTACAACGCCATCACCAATGGCACACCCTGCGTG
  ATAGTGGAGGGCTCCGGCCGAGTGGCTGACGTCATCGCTCAGGTGGCCGCTCTGCCCGTCTCTGAGATCA
  CCATCTCCCTGATCCAGCAGAAGCTCAGCGTCTTCTTCCAGGAGATGTTTGAGACTTTCACCGAAAACCA
  GATTGTGGAATGGACCAAAAAGATCCAAGATATTGTCAGGAGGCGGCAGCTGCTGACGGTCTTCCGGGAA
  GGCAAGGATGGTCAGCAGGATGTGGATGTTGCCATTCTGCAAGCTTTACTGAAAGCCTCTCGAAGCCAAG
  ATCACTTCGGCCACGAGAACTGGGACCATCAGCTGAAGCTGGCCGTGGCCTGGAACCGTGTGGACATCGC
  CCGCAGTGAGATCTTCACTGATGAGTGGCAGTGGAAGCCTTCAGACCTGCATCCCATGATGACAGCTGCC
  CTCATCTCCAACAAGCCTGAGTTTGTGAGGCTCTTTCTGGAGAACGGGGTGCGGCTGAAGGAGTTTGTCA
  CCTGGGATACTCTTCTCTGCCTCTACGAGAACCTGGAGCCATCCTGCCTTTTCCACAGCAAGCTGCAGAA
  GGTGCTGGCAGAAGAGCATGAACGCTTAGCCTATGCATCTGAGACACCCCGGCTGCAAATGCACCACGTG
  GCCCAGGTGCTGCGTGAGCTCCTCGGAGACTCCACACAGCTGCTGTATCCCCGGCCCCGGTACACTGACC
  GGCCACGGCTCTCGCTGCCCATGCCACACATCAAACTCAACGTGCAAGGAGTGAGCCTCCGGTCTCTCTA
  TAAGCGATCAACAGGCCACGTTACCTTCACCATTGACCCAGTCCGCGATCTTCTCATTTGGGCCATCATC
  CAGAACCACAGGGAGCTGGCGGGCATCATCTGGGCTCAGAGCCAGGACTGCACAGCAGCCGCACTGGCCT
  GCAGCAAGATCCTGAAGGAGCTGTCCAAGGAGGAGGAAGATACAGACAGCTCTGAGGAGATGCTGGCACT
  CGCGGATGAGTTTGAGCATAGAGCTATCGGTGTCTTCACCGAGTGCTACAGAAAGGATGAGGAAAGAGCC
  CAGAAGCTGCTTGTCCGTGTGTCTGAGGCCTGGGGGAAGACCACCTGCCTGCAGCTGGCCCTCGAGGCCA
  AGGACATGAAATTCGTGTCTCACGGAGGGATCCAGGCTTTCCTAACGAAGGTGTGGTGGGGTCAGCTCTG
  CGTGGACAATGGCCTGTGGAGGATCATCCTGTGCATGCTGGCCTTCCCTCTGCTCTTCACCGGCTTCATC
  TCCTTCAGGGAAAAGAGGCTGCAGGCACTGTGCCGCCCGGCCCGCGTCCGCGCCTTCTTCAACGCGCCGG
  TGGTCATCTTCTACCTCAATATTCTCTCCTACTTTGCCTTCCTCTGCCTGTTTGCCTACGTGCTCATGGT
  GGACTTCCAGCCCTCACCATCCTGGTGCGAGTACCTCATCTACCTGTGGCTCTTCTCCCTGGTGTGCGAG
  GAGACACGGCAGCTATTCTACGATCCCGATGGCTGCGGGCTCATGAAGATGGCGTCCCTGTACTTCAGTG
  ACTTCTGGAACAAACTGGACGTTGGGGCCATTCTGCTCTTTATAGCAGGACTGACCTGCCGACTCATCCC
  AGCGACGCTGTACCCTGGGCGCATCATCCTGTCTTTGGACTTCATTATGTTCTGCCTCCGCCTCATGCAC
  ATCTTCACCATTAGCAAGACACTGGGGCCCAAGATAATCATCGTGAAGCGGATGATGAAGGACGTCTTCT
  TCTTCCTCTTTCTCCTGGCGGTGTGGGTGGTGTCCTTCGGAGTGGCCAAGCAGGCCATCCTCATCCACAA
  TGAGAGCCGCGTGGACTGGATCTTCCGCGGAGTTATCTATCACTCTTACCTTACCATCTTCGGGCAGATC
  CCGACCTACATTGACGGCGTGAATTTCAGCATGGACCAGTGCAGCCCCAATGGTACAGACCCCTACAAGC
  CCAAGTGTCCTGAGAGTGACTGGACAGGGCAGGCACCCGCCTTCCCCGAGTGGCTGACAGTCACCCTTCT
  CTGCCTCTACCTGCTCTTCGCCAACATCCTGCTGCTTAATTTGCTCATCGCCATGTTCAACTACACCTTC
  CAGGAGGTGCAGGAGCACACAGACCAGATCTGGAAGTTCCAGCGCCACGACCTGATTGAGGAGTACCACG
  GCCGGCCCCCGGCCCCTCCCCCACTCATCCTCCTCAGCCACCTGCAGCTCCTGATCAAGAGGATTGTCCT
  GAAGATCCCCGCCAAGAGGCACAAGCAGCTCAAGAACAAGCTGGAGAAGAATGAGGAGGCAGCCCTCCTG
  TCCTGGGAGCTCTACCTGAAGGAGAATTACCTGCAGAACCAACAGTACCAGCACAAACAGCGGCCAGAGC
  AGAAGATCCAGGACATCAGTGAGAAAGTGGACACCATGGTGGATCTGTTGGACATGGACCGTGTGAAGAG
  ATCAGGCTCCACAGAGCAGAGGCTGGCCTCCCTTGAGGAACAGGTGACTCAGATGGGCAGATCTTTGCAC
  TGGATCGTGACGACCCTGAAGGACAGTGGCTTTGGCTCAGGGGCCGGTGCACTGACCCTGGCAGCCCAAA
  GGGCCTTCGACGAGCCAGATGCTGAGCTGAGTATCAGGAAGAAAGGAGAGGAGGGAGGAGATGGCTATCA
  TGTGAGCGCCCGGCACCTCCTCTACCCTGATGCCCGCATCATGCGCTTCCCCGTGCCTAATGAGAAGGTG
  CCTTGGGAGGCAGAGTTTCTGATCTACGACCCTCCGTTTTACACAGCTGAGAAGAAGGATGCGACTCTCA
  CAGACCCTGTGGGAGACACTGCAGAACCTCTGTCTAAGATCAATTACAACGTCGTGGACGGACTGATGGA
  CCGTTGCAGCTTCCATGGGACCTATGTGGTCCAATATGGATTCCCTTTGAACCCCATGGGCCGCACCGGG
  TTGCGTGGTCGTGGGAGCCTCAGCTGGTTTGGTCCCAACCACACTCTGCAGCCAGTTGTTACCCGGTGGA
  AGAGGAACCAGGGTGGAGGCATCTGCCGGAAGAGTGTCAGGAAGATGTTGGAGGTGCTGGTCATGAAGTT
  GCCTCAATCCGAGCACTGGGCCTTGCCTGGGGGCTCTCGGGAGCCAGGGAAGATGCTACCACGGAAGCTG
  AAACAGGTCCTCCAGCAGGAGTACTGGGTGACCTTTGAGACCTTGCTAAGGCAAGGTACAGAGGTGTACA
  AAGGATACGTGGATGACCCAAGGAACACGGACAATGCCTGGATCGAGACGGTGGCTGTCAGCATCCATTT
  CCAGGACCAGAATGATGTGGAGCTGAAGAGGCTGGAAGAGAACCTGCAAACTCATGATCCAAAGGAGTCG
  GCCCGTGGCTTGGAGATGTCTACTGAATGGCAGGTTGTAGACCGGCGGATCCCTCTGTATGTGAACCACA
  AGAAGATCCTCCAAAAGGTGGCCTCGCTGTTTGGCGCTCACTTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001011559
• Insert Size: 4527 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001011559.1, NP_001011559.1
• RefSeq Size: 4527 bp
• RefSeq ORF: 4527 bp
• Locus ID: 294329
• UniProt ID: E9PTA2
• Cytogenetics: 20p12
• Gene Summary: Nonselective, voltage-independent cation channel that mediates Na(+) and Ca(2+) influx, leading to increased cytoplasmic Ca(2+) levels (PubMed:16651700, PubMed:16260005, PubMed:11804595, PubMed:16601673, PubMed:19454650). Functions as ligand-gated ion channel. Binding of ADP-ribose to the cytoplasmic Nudix domain causes a conformation change; the channel is primed but still requires Ca(2+) binding to trigger channel opening. Extracellular calcium passes through the channel and increases channel activity (By similarity). Also contributes to Ca(2+) release from intracellular stores in response to ADP-ribose (PubMed:19454650). Plays a role in numerous processes that involve signaling via intracellular Ca(2+) levels (Probable). Besides, mediates the release of lysosomal Zn(2+) stores in response to reactive oxygen species, leading to increased cytosolic Zn(2+) levels (PubMed:25562606). Activated by moderate heat (35 to 40 degrees Celsius) (PubMed:16601673). Activated by intracellular ADP-ribose, beta-NAD (NAD(+)) and similar compounds, and by oxidative stress caused by reactive oxygen or nitrogen species (PubMed:16260005, PubMed:16601673, PubMed:25562606). The precise physiological activators are under debate; the true, physiological activators may be ADP-ribose and ADP-ribose-2'-phosphate. Activation by ADP-ribose and beta-NAD is strongly increased by moderate heat (35 to 40 degrees Celsius) (By similarity). Likewise, reactive oxygen species lower the threshold for activation by moderate heat (37 degrees Celsius). Plays a role in mediating behavorial and physiological responses to moderate heat and thereby contributes to body temperature homeostasis. Plays a role in insulin secretion, a process that requires increased cytoplasmic Ca(2+) levels (PubMed:16601673). Required for normal IFNG and cytokine secretion and normal innate immune immunity in response to bacterial infection. Required for normal phagocytosis and cytokine release by macrophages exposed to zymosan (in vitro). Plays a role in dendritic cell differentiation and maturation, and in dendritic cell chemotaxis via its role in regulating cytoplasmic Ca(2+) levels (By similarity). Plays a role in the regulation of the reorganization of the actin cytoskeleton and filopodia formation in response to reactive oxygen species via its function in increasing cytoplasmic Ca(2+) and Zn(2+) levels (By similarity). Confers susceptibility to cell death following oxidative stress (PubMed:16651700, PubMed:11804595, PubMed:19454650, PubMed:25562606).[UniProtKB/Swiss-Prot Function]