Hsd3b7 (NM_139329) Rat Untagged Clone

CAT#: RN204318

Hsd3b7 (untagged ORF) - Rat hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7 (Hsd3b7), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Hsd3b7
• Synonyms: Cca2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN204318 representing NM_139329
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGACTCTGCACAGGTGCCAGCACTGGTATACCTGGTCACAGGTGGCTGTGGCTTCCTGGGGGAGC
  ATATTGTTCGAATGCTGCTGGAGTGGGAGCCCAGGCTCCGGGAGCTGCGTGTCTTTGACCTGCACCTGAG
  TTCTTGGCTGGAGGAGCTGAAAACAGGGCCTGTGCAGGTGACTGCCATCCAGGGGGATGTGACTCAGGCC
  CACGAGGTGGCAGCAGCCATGGCTGGATCTCATGTGGTCATCCATACAGCTGGGCTGGTAGATGTGTTTG
  GGAAGGCCAGTCCAGAGACCATCCACAAAGTCAATGTGCAGGGCACACAGAATGTGATTGACGCTTGTGT
  GCAGACCGGGACTCGGCTCCTGGTCTACACGAGTAGCATGGAAGTGGTAGGGCCTAATGTCAAGGGCCAC
  CCCTTCTACAGGGGCAATGAAGACACCCCATATGAGGCCATCCACAGGCATCCCTACCCATGTAGCAAGG
  CCCTCGCTGAGCAGTTGGTCCTCGAGGCCAATGGAAGGAAGGGACTGCGCTTTGGAGGTCGGCTGTTTCG
  GGCCATCCCAGCTTCCGTGGAGCATGGTCGGGTCTACGTTGGCAATGTGGCTTGGATGCATATACTGGTG
  GCCCGAGAGCTGGAGCAGCGGGCAGCACTCATGGGTGGCCAGGTGTATTTCTGCTATGATAAGTCACCTT
  ATAAAAGCTACGAGGACTTCAACATGGAGTTTCTGAGTCCCTGTGGACTTCGGCTGATAGGCACCCACCC
  ACTGCTGCCCTACTGGCTACTGGTGCTGCTGACTGCCCTCAACGCTCTGCTGCAGTGGCTGCTTCGGCCG
  CTGGTGCTGTACACACCCCTGCTGAATCCCTACACTCTGGCTGTGGCCAACACCACCTTTACTGTCAGCA
  CCAACAAGGCACAGCGCCATTTCGGCTACAAGCCCCTCTTCTCATGGGAAGAGAGCAGGGCCCGCACCAT
  TCACTGGGTGCAGGCCATGGAGGGTTCCGCCTGGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_139329
• Insert Size: 1017 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_139329.1, NP_647545.1
• RefSeq Size: 1795 bp
• RefSeq ORF: 1017 bp
• Locus ID: 246211
• UniProt ID: O35048
• Cytogenetics: 1q37
• Gene Summary: protein that is expressed in under growth-arrest conditions [RGD, Feb 2006]