Cdk1 (NM_019296) Rat Untagged Clone

CAT#: RN202805

Cdk1 (untagged ORF) - Rat cell division cycle 2, G1 to S and G2 to M (Cdc2), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Cdk1
• Synonyms: Cdc2; Cdc2a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN202805 representing NM_019296
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGACTATATCAAAATAGAGAAAATCGGAGAAGGGACTTATGGTGTGGTATATAAAGGTAGACACA
  GGACTACTGGCCAGATCGTGGCCATGAAGAAAATCAGACTTGAAAGCGAGGAAGAAGGAGTGCCCAGTAC
  GGCAATCCGGGAAATCTCTCTGTTAAAAGAACTTCGACATCCAAATATAGTCAGCCTGCAGGATGTACTC
  ATGCAGGACTCCAGGCTGTATCTCATCTTCGAGTTCCTGTCCATGGATCTCAAGAAGTACCTGGACTCTA
  TCCCTCCTGGCCAGTTCATGGATTCTTCGCTCGTTAAGAGTTACTTGTACCAAATCCTCCAGGGGATTGT
  GTTTTGTCACTCCCGACGAGTTCTTCACAGAGACTTGAAACCTCAAAATCTATTGATTGATGACAAAGGA
  ACAATCAAACTGGCAGATTTCGGCCTTGCCAGAGCGTTTGGAATACCGATAAGAGTGTACACACACGAGG
  TAGTGACGCTGTGGTACCGGTCGCCAGAGGTGTTGCTGGGCTCGGCTCGCTACTCCACCCCGGTTGACAT
  CTGGAGCATAGGGACCATATTTGCAGAGCTGGCGACCAAGAAGCCGCTTTTCCACGGCGACTCAGAGATT
  GACCAGCTCTTCAGGATCTTCAGAGCTCTGGGCACCCCTAACAACGAAGTGTGGCCAGAAGTAGAGTCCC
  TGCAGGACTACAAGAACACTTTCCCCAAGTGGAAGCCGGGGAGCCTCGCGTCCCACGTCAAGAACCTGGA
  TGAAAACGGCTTGGACTTGCTCTCGAAAATGCTGGTCTATGATCCAGCCAAACGAATCTCTGGAAAAATG
  GCCCTTAAGCACCCATACTTTGACGACTTGGACAATCAGATCAAGAAGATGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_019296
• Insert Size: 894 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019296.1, NP_062169.1
• RefSeq Size: 1184 bp
• RefSeq ORF: 894 bp
• Locus ID: 54237
• UniProt ID: P39951
• Cytogenetics: 20p11
• Gene Summary: putative cell cycle control protein; homologous to S. pombe Cdc2 [RGD, Feb 2006]