Chd8 (NM_022933) Rat Untagged Clone

CAT#: RN202127

Chd8 (untagged ORF) - Rat chromodomain helicase DNA binding protein 8 (Chd8), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Chd8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN202127 representing NM_022933
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGACCCCATCATGGATCTGTTTGATGACCCAAACTTATTTGGCCTGGATTCTTTGACTGATGACA
  GCTTTAATCAGGTCACTCAAGACCCCATTGAAGAAGCACTTGGACTGCCAAGTTCTCTGGACTCCTTGGA
  TCAGATGAACCAAGATGGTGGAAGTGGTGATGTGGGGAATTCATCAGCAAGTGACCTGGTTCCCCCACCA
  GAGGAAACAGCTTCCACTGAACTTCCCAAAGAATCCACAGCTCCAGCTCCAGAATCCTTAACCTTGCATG
  ATTATACCACTCAGCCCACCAGCCAGGAGCAGCCAGCCCAACCTGTCTTGCAGACATCAACACCAACATC
  AGGACTTTTGCAGGTCTCTAAGAGCCAGGAGATCCTGAGCCAAGGGAATCCTTTCATGGGTGTCTCTGCC
  ACAGCTGTCTCCCCCAGTAATACTGGAGGACAGCCATCTCAGTCAGCTCCTAAGATTGTTATTCTTAAAG
  CTCCACCAAACTCCTCAGTCACAGGTGCCCATGTGGCACAAATTCAGGCCCAAGGTATCACCAGTACAGC
  TCAGCCCCTAGTGGCTGGCACAGCCAATGGTGGAAAAGTCACTTTTACCAAAGTGCTTACTGGTACACCC
  CTCCGACCAGGTGTATCCATTGTCTCTGGTAATACAGTGCTGGCCACGAAGGTCCCTGGGAACCAGGCTG
  CTGTTCAGCGCATTGTCCAGCCAAGCCGACCAGTAAAGCAGCTAGTTCTCCAGCCAGTTAAGGGTTCCGC
  TCCTGCTGGAAATCCCGGGGCTACCGGGCCCCCCCTGAAGCCTGCAGTTACACTGACATCTACACCTGCC
  CAGGGTGAATCGAAACGCATCACTTTGGTCCTTCAGCAGCCACAGTCCGGAGGTCCCCAAGGACATCGGC
  ATGTTGTGTTAGGGAGTCTACCCGGCAAGATAGTGTTACAGGGCAACCAACTGGCAGCCTTGACTCAAGC
  CAAGAGTGCTCAGGGGCAGCCTGCCAAAGTAGTCACCATCCAACTGCAGGTGCAGCAGCCACAGCAAAAG
  ATCCAGATTGTACCACAGCCCCCATCATCACAGCCACAGCCACAGCCGCCACCCTCAGCCCAACCATTGA
  CCCTGTCCTCTGTACAGCAGGCTCAGATAATGGGACCAGGCCAAAACCCAGGACAGAGACTTTCTGTACC
  GCTCAAGATGGTGCTGCAGCCACAGGCTGGCTCTTCTCAGGGAGCCTCTTCTGGACTCTCTGTGGTTAAA
  GTTCTAAGTGCCAGTGAAGTGGCAGCCCTGTCATCACCAGCAAGCTGTGCCCCTCACACTGCAGGGAAGA
  CTGGAATGGAGGAGAACCGCAGGCTTGAGCACCAGAAAAAGCAAGAAAAAGCAAATCGGATTGTGGCAGA
  GGCCATTGCTAGGGCCCGGGCCAGGGGTGAGCAAAACATACCTCGAGTCCTGAATGAGGATGAGCTGCCT
  AGTGTCCGGCCAGAAGAGGAGGGTGAAAAGAAACGCAGGAAGAAGAGCAGTGGAGAAAGGCTGAAGGAAG
  AGAAGCCAAAGAAAAGCAAGACTGCTGCTGCCTCCAAAACAAAGGGCAAGAGTAAGCTAAACACCATCAC
  TCCTGTAGTGGGCAAAAAGAGAAAGCGTAACACATCGTCTGACAACTCTGATGTGGAGGTCATGCCTGCA
  CAGTCGCCCCGGGAGGACGAAGAGAGCAGCATCCAGAAGAGACGTTCAAACCGCCAAGTTAAGCGAAAAA
  AATATACAGAGGACCTGGATATAAAGATAACAGATGATGAAGAGGAGGAGGAGGTCGATGTTACTGGTCC
  AATAAAACCCGAGCCCATCCTCCCAGAACCAGTGCCAGAGCCTGATGGGGAGACTCTGCCTTCCATGCAG
  TTCTTTGTGGAGAATCCCAGTGAAGAAGACGCAGCTATTGTGGACAAAGTGCTTTCCATGCGAGTTGTGA
  AGAAAGAGCTTCCTTCTGGACAGTACACTGAGGCTGAGGAATTCTTTGTCAAGTACAAGAACTACTCCTA
  TCTGCACTGTGAATGGGCAACTATCTCCCAGCTGGAGAAGGACAAGAGGATCCATCAGAAGCTAAAGCGC
  TTCAAGACGAAAATGGCTCAGATGAGGCACTTCTTTCATGAAGATGAAGAGCCTTTCAATCCAGACTACG
  TCGAGGTGGACAGGATACTGGACGAGTCTCACAGTGTGGACAAGGATAATGGTGAGCCTGTAATCTACTA
  CCTGGTAAAATGGTGCTCTCTGCCCTATGAGGACAGTACGTGGGAGCTAAAAGAGGATGTTGACGAGGGC
  AAGATTCGGGAATTTAAACGGATCCAGTCAAGGCATCCAGAACTCAAAAGGGTGAATCGTCCACAGGCAA
  ATGCCTGGAAGAAATTGGAGTTGTCACATGAATATAAAAACAGAAACCAATTACGGGAGTATCAGTTAGA
  AGGGGTCAACTGGCTTCTCTTTAATTGGTATAACAGGCAGAACTGCATCCTGGCTGATGAAATGGGATTA
  GGCAAAACCATTCAGTCCATTGCCTTCTTGCAGGAGGTATATAATGTGGGCATCCATGGTCCCTTTTTGG
  TCATTGCTCCATTGTCCACCATTACTAACTGGGAGCGAGAATTTAATACCTGGACAGAGATGAACACTAT
  TGTGTACCATGGCAGCCTGGCCAGCCGGCAGATGATTCAGCAATATGAAATGTACTGTAAAGATTCACGG
  GGTCGCCTCATCCCAGGTGCATATAAGTTTGATGCCTTGATCACCACTTTTGAGATGATTTTGTCAGACT
  GTCCTGAACTTCGTGAAATTGAATGGCGTTGTGTTATCATTGATGAAGCCCATCGGCTAAAGAACCGTAA
  TTGTAAGCTACTCGATAGCCTCAAGCACATGGACCTGGAGCATAAAGTGTTACTTACAGGAACACCATTG
  CAAAATACTGTAGAGGAGCTGTTCAGCCTACTTCATTTCTTGGAACCTTCTCAGTTTCCCTCAGAATCAG
  AATTCCTTAAGGATTTTGGGGATCTGAAGACAGAGGAGCAGGTTCAAAAGCTTCAGGCCATTCTAAAGCC
  AATGATGCTGAGAAGACTCAAAGAGGATGTTGAAAAGAATTTGGCTCCCAAACAGGAGACTATTATTGAA
  GTAGAGCTGACCAACATCCAGAAGAAATACTACAGAGCTATTTTAGAGAAGAATTTCTCCTTCCTTTCCA
  AAGGAGCAGGGCATACCAATATGCCTAACCTCCTGAACACGATGATGGAGTTACGCAAGTGCTGCAACCA
  CCCATACCTCATCAATGGTGCAGAAGAGAAAATCCTGATGGAATTTCGGGAAGCTTGCCATATTATACCT
  CAAGATTTCCATCTGCAGGCCATGGTTCGGTCAGCTGGCAAATTAGTTCTTATTGACAAGTTACTTCCAA
  AGCTTAAAGCTGGTGGCCATAAAGTTCTGATCTTCTCCCAGATGGTACGCTGTCTAGATATTCTGGAGGA
  TTATCTGATCCAGAGGAGGTACTTATACGAACGCATTGATGGGCGAGTTAGAGGCAACCTTCGACAAGCT
  GCTATCGACCGCTTTAGCAAGCCTGACTCAGACCGCTTTGTCTTCTTGCTATGTACCCGTGCTGGTGGAC
  TTGGTATTAATCTTACAGCTGCTGATACCTGTATTATTTTTGATTCAGACTGGAATCCACAGAATGACCT
  GCAGGCCCAAGCACGTTGTCATCGCATTGGACAGAGCAAAGCTGTAAAGGTGTACCGACTCATCACTCGC
  AATTCCTACGAGAGAGAGATGTTTGATAAAGCTAGCCTCAAGTTGGGGCTGGACAAAGCTGTGCTTCAGT
  CCATGAGTGGTCGGGATGGCAACATTACTGGAATTCAACAGTTCTCCAAGAAAGAGATTGAAGATCTCTT
  AAGGAAAGGTGCATATGCAGCCATAATGGAGGAAGACGATGAGGGTTCTAAGTTTTGTGAAGAGGACATA
  GATCAGATCTTGCTGAGACGGACTACAACCATCACTATTGAGTCTGAAGGGAAAGGGTCTACTTTTGCCA
  AGGCAAGCTTTGTTGCTTCTGAAAATAGGACAGATATTTCCCTGGATGATCCAAACTTTTGGCAAAAATG
  GGCCAAAAAGGCAGACCTAGACATGGATCTGCTGAATAGTAAGAATAACTTAGTGATTGATACACCACGA
  GTTCGGAAACAGACTCGACACTTCAGCACTCTAAAAGATGACGACCTGGTTGAGTTTTCTGATTTAGAAA
  GTGAAGATGATGAACGTCCCCGTTCCCGAAGACATGACCGTCATCACACCTATGGGCGCACTGACTGCTT
  TCGGGTAGAAAAACATCTCCTGGTATATGGTTGGGGACGTTGGAGAGATATTCTGTCTCATGGACGATTT
  AAGCGGCGCATGACTGAAAGAGATGTGGAGACAATATGCCGAGCCATCCTTGTGTACTGTCTTCTACACT
  ACCGTGGGGATGAAAACATCAAAAGCTTCATTTGGGACTTGATTAGCCCTGCTGAAAATGGCAAGACAAA
  AGAATTGCAGAATCATTCAGGTCTGTCTATCCCTGTGCCCCGTGGGCGTAAGGGGAAAAAAGTGAAGTCA
  CAAAGTACTTTTGACATTCATAAGGCAGACTGGATCCGGAAATATAACCCTGATACTCTGTTCCAAGACG
  AGAGCTATAAGAAGCACTTGAAACATCAGTGTAACAAGGTACTGTTGAGAGTACGGATGCTGTACTACCT
  GAGACAGGAGGTCATTGGAGACCAAGCAGAGAAGGTGTTAGGGGGCGCCATAGCCAGTGAGATTGACATA
  TGGTTCCCAGTAGTGGATCAGCTGGAGGTTCCAACAACATGGTGGGATAGTGAGGCTGACAAATCCCTGC
  TCATTGGCGTTTTTAAGCATGGCTATGAAAAATACAATACCATGAGGGCAGACCCTGCCTTGTGTTTCCT
  GGAAAAAGCTGGCAGGCCAGATGACAAAGCCATTGCAGCGGAGCACAGAGTTCTGGATAACTTCTCCGAC
  CTGGTAGAAGGGATTGACTTTGATAAGGATTGTGAAGATCCTGAATATAAACCCCTTCAGGGTCCCCCAA
  AGGACCCGGATGATGAGGGTGATCCCTTGATGATGATGGATGAGGAGATCTCAGTCATTGACGGAGATGA
  AGCCCCGGTGACTCAACAGCCAGGGCATTTATTCTGGCCTCCAGGCTCTGCCCTCACAGCTAGGCTCCGC
  CGCCTGGTAACCGCCTATCAACGCAGCTACAAGAGAGAACAAATGAAGATAGAGGCTGCGGAGCGTGGGG
  ACCGGAGACGCCGACGTTGTGAAGCGGCCTTTAAGCTCAAAGAAATTGCAAGGCGGGAAAAACAACAACG
  ATGGACACGGCGCGAGCAAACTGATTTCTATCGAGTAGTGTCTACCTTTGGGGTAGAGTACGACCCTGAC
  AACATGCAGTTTCACTGGGATCGCTTCCGTACTTTTGCTCGCCTGGACAAAAAAACAGATGAAAGCCTTA
  CCAAGTACTTCCATGGTTTTGTGGCCATGTGTAGACAAGTGTGCCGCCTTCCCCCAGCTGCTGGTGATGA
  ACCTCCAGATCCTAATCTGTTTATTGAGCCCATCACTGAAGAGAGAGCATCACGGACTCTCTACCGAATC
  GAGTTGCTTCGACGCTTACGGGAACAAGTTTTATGCCACCCCCTTTTAGAAGATCGGCTGGCATTGTGTC
  AGCCTCCAGGTCTTGAATTGCCCAAATGGTGGGAACCTGTCCGTCACGATGGGGAGCTCCTCCGAGGAGC
  AGCCCGCCATGGGGTGAGCCAAACAGACTGCAACATCATGCAGGACCCAGACTTCTCTTTTCTGGCTGCC
  CGTATGAATTATATGCAGAACCATCAGGCAGGAGCATCAGCTGCATCCCTGTCACGATGCTCCACTCCAC
  TGCTGCACCAGCAGTGTACCTCACGCACTGCTTCACCATCACCCCTGCGCCCAGATGTTCCTGCTGAAAA
  GTCACCTGAGGAGAATGCTGTTCAGGTCCCCAGTCTGGACAGTCTGACTTTAAAACTAGAGGATGAGGTG
  GTGGCTAGGAGCCGACTCACCCCACAAGACTATGAAATTCGTGTAGCCTCTTCAGATACAGCCCCTCTGT
  CCCGGAGTGTTCCGCCAGTCAAACTGGAGGATGACGATGATTCAGACTCTGAGCTGGACTTGAGCAAGTT
  GTCACCATCGTCCTCTTCATCTTCATCCTCCTCCAGCTCCAGCTCCAGCAGTGATGAGAGTGAAGATGAG
  AAGGAAGAGAAGTTAACTGCTGACCGGTCCCGCCCAAAGCTCTATGATGAAGAGAGCCTTCTGTCCCTTA
  CTATGTCCCAAGATGGATTCCCAAATGAAGATGGAGAACAAATGACCCCTGAGTTGCTGCTGCTGCAGGA
  AAGACAAAGAGCCTCTGAATGGCCCAAGGATCGTGTTCTGATAAACCGCATTGACCTCGTCTGCCAGGCT
  GTACTCTCAGGGAAATGGCCTTCTAACCGCCGGAGCCAGGAAATGACAACAGGAGGAATTTTGGGGCCAG
  GAAACCATTTATTAGACAGTCCCTCTTTGACCCCAGGAGAATATGGGGACTCTCCGGTCCCCACACCACG
  AAGCAGCAGTGCAGCTTCCATGGTAGAGGAAGAAGCATCTGCGGTCACCACAGCAGCAGCCCAATTTACT
  AAACTTCGGCGAGGCATGGACGAGAAAGAGTTTACAGTTCAGATCAAAGATGAAGAAGGATTGAAGTTAA
  CATTCCAGAAGCATAGATTGATGGCTAATGGTGTAATGGGAGATGGACATCCACTGTTTCATAAGAAGAA
  GGGGAACAGGAAAAAGCTAGTAGAGCTGGAGGTGGAGTGCATGGAAGAGCCTAATCACCTTGATGTGGAC
  CTGGAGACCCGGATCCCTGTCATCAATAAGGTGGATGGTACTTTGCTGGTGGGTGATGAGGCCCCTCGCC
  GGGCGGAGCTGGACATGTGGTTACAGGGTCATCCAGAGTTTGCTGTTGATCCCCGATTTCTAGCGTATAT
  GGAGGAACGTAGAAAACAGAAGTGGCAGAGATGTAAAAAAAATAATAAGACAGAATTGAACTGTTTGGGA
  ATGGAACCAGTCCAGCCAGCCAACTCTAGAAATGGGAAAAAGGGTCACTATGCTGAAACTGCGTTCAACC
  GGGTTTTGCCAGGGCCTATTGCACCAGAAAACAGCAAGAAACGGGTCCGCAGGACAAGACCAGACCTTTC
  TAAGATGATGGCCCTGATGCAGGGTGGAAGCACTGGGTCCCTATCTCTGCATAACACCTTTCAACACAGC
  AGTAGTAACCTACAGTCTGTGTCGTCTCTGGGTCACAGCAGTGCCACTTCTGCATCTCTGCCTTTCATGC
  CGTTTGTGATGGGTGGTGCAGCAGCACCCCCTCATGTAGACTCCAGCACCATGCTTCATCATCACCACCA
  CCACCCCCACCCCCACCATCACCACCATCACCATCCAGGCTTGAGAACCACTGGCTACCCTTCTTCACCA
  GCTACTACCACCTCTGGTACTGCCTTGCGGTTACCAACACTGCAGCATGAGGACGATGATGAAGAGGAGG
  ATGAAGATGATGATGATTTATCTCAGGGCTATGATAGCTCAGAAAGGGACTTCTCACTCATTGATGACCC
  TATGATGCCAGCCAACTCAGACTCCAGTGATGATGCTGACGACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_022933
• Insert Size: 7746 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022933.2, NP_075222.2
• RefSeq Size: 8425 bp
• RefSeq ORF: 7746 bp
• Locus ID: 65027
• Cytogenetics: 15p14
• Gene Summary: This gene encodes a member of the chromodomain-helicase-DNA binding protein family, which is characterized by a SNF2-like domain and two chromatin organization modifier domains. The encoded protein also contains brahma and kismet domains, which is common to the subfamily of chromodomain-helicase-DNA binding proteins to which this protein belongs. In mammals, this gene has been shown to function in several processes including transcriptional regulation, epigenetic remodeling, promotion of cell proliferation, and regulation of RNA synthesis. Knockout of this gene in mice causes early embryonic lethality due to widespread apoptosis. Heterozygous loss of function mutations in mice result in autism spectrum disorder-like behaviors that include increased anxiety, repetitive behavior, and altered social behavior. [provided by RefSeq, Dec 2016]