Camk2a (NM_012920) Rat Untagged Clone

CAT#: RN201121

Camk2a (untagged ORF) - Rat calcium/calmodulin-dependent protein kinase II alpha (Camk2a), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Camk2a
• Synonyms: PK2CDD; PKCCD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN201121 representing NM_012920
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTACCATCACCTGCACCCGATTCACGGAAGAGTACCAGCTCTTCGAGGAACTGGGAAAGGGAGCCT
  TCTCCGTGGTGCGCAGGTGTGTGAAGGTGCTGGCTGGCCAGGAGTATGCTGCCAAGATTATCAACACCAA
  GAAGCTCTCAGCCAGAGATCACCAGAAGTTGGAACGCGAGGCCCGCATCTGCCGCTTGTTGAAGCACCCC
  AATATCGTCCGACTCCATGACAGCATCTCCGAGGAGGGGCACCACTACCTTATCTTCGATCTGGTCACTG
  GTGGGGAGCTGTTCGAAGACATTGTGGCCCGGGAGTATTACAGTGAGGCTGATGCCAGCCACTGTATCCA
  GCAGATCCTGGAGGCTGTGCTACACTGTCACCAGATGGGGGTGGTGCATCGCGACCTGAAGCCTGAGAAT
  CTGTTGCTGGCTTCGAAGCTCAAGGGTGCTGCGGTGAAGCTGGCAGACTTTGGCCTGGCCATAGAGGTTG
  AGGGAGAGCAGCAGGCATGGTTTGGGTTCGCAGGGACACCTGGATACCTCTCCCCAGAAGTGCTGCGGAA
  GGACCCATACGGGAAGCCTGTGGACCTGTGGGCCTGTGGCGTCATCCTGTATATCTTGCTGGTTGGGTAT
  CCCCCATTCTGGGATGAGGACCAGCACCGCCTGTACCAGCAGATCAAAGCTGGTGCCTACGATTTCCCAT
  CACCAGAATGGGACACCGTCACCCCGGAAGCCAAGGATCTGATCAATAAGATGCTGACCATCAACCCGTC
  CAAACGCATCACGGCCGCTGAGGCTCTCAAGCACCCCTGGATCTCGCACCGCTCCACTGTGGCCTCCTGC
  ATGCACAGACAGGAGACCGTGGACTGCCTGAAGAAGTTCAATGCCAGGAGGAAACTGAAGGGAGCCATCC
  TCACCACTATGCTGGCCACCAGGAACTTCTCCGGAGGGAAGAGTGGAGGAAACAAGAAGAATGATGGCGT
  GAAGGAATCCTCTGAGAGCACCAACACCACCATCGAGGATGAAGACACCAAAGTGCGCAAACAGGAAATT
  ATCAAAGTGACAGAGCAGCTGATCGAAGCCATAAGCAATGGAGACTTTGAATCCTACACGAAGATGTGCG
  ACCCTGGAATGACAGCCTTTGAACCGGAGGCCCTGGGGAACCTGGTCGAGGGCCTGGACTTTCATCGATT
  CTATTTTGAAAACCTGTGGTCCCGGAACAGCAAGCCCGTGCACACCACCATCCTGAACCCTCACATCCAC
  CTGATGGGTGACGAGTCAGCCTGCATCGCCTACATCCGCATCACTCAGTACCTGGATGCGGGTGGCATCC
  CCCGCACGGCCCAGTCAGAGGAGACCCGTGTCTGGCACCGCAGGGATGGAAAATGGCAGATCGTCCACTT
  CCACAGATCTGGGGCGCCCTCCGTCCTGCCCCATTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_012920
• Insert Size: 1437 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012920.1, NP_037052.1
• RefSeq Size: 1510 bp
• RefSeq ORF: 1437 bp
• Locus ID: 25400
• UniProt ID: P11275
• Cytogenetics: 18q12.1
• Gene Summary: Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in synaptic plasticity, neurotransmitter release and long-term potentiation. Member of the NMDAR signaling complex in excitatory synapses, it regulates NMDAR-dependent potentiation of the AMPAR and therefore excitatory synaptic transmission (PubMed:15312654). Regulates dendritic spine development. Also regulates the migration of developing neurons. Phosphorylates the transcription factor FOXO3 to activate its transcriptional activity (By similarity).[UniProtKB/Swiss-Prot Function]