Bsg (NM_001109882) Rat Untagged Clone

CAT#: RN200443

Bsg (untagged ORF) - Rat basigin (Bsg), transcript variant 1, (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Bsg
• Synonyms: 5A11; basignin; CE9; EMMPRIN; HT7; Ox47R
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN200443 representing NM_001109882
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGCGCTGCTGCTGGCGCTGGCCTTCACGTTCCTGAGTGGCCAAGGCGCCTGCGCGGCGGCTG
  GCTTTCTCAAGGCGCCAATGTCCCAGGAGCAGTGGGCGGGGGGCAGCGTGGTCCTGCACTGTGAGGCTGT
  GGGCAGCCCCATGCCGGAGATCCAGTGGTGGTTTGAAGGGAATGAACCAAACGACAGCTGCTCCCAGCTC
  TGGGATGGCGCCCGGCTGGACCGTGTTCACATCCATGCCACCTACCGCCAGCATGCAGCCAGCACGCTGT
  CTGTTGATGGGCTCGCCGCAGAGGACACAGGCACTTATGAATGCCGGGCCAGCAGTGACCCTGACCGCAA
  CCACCTGACCCGGCCACCCAGGGTCAAGTGGGTTCGTGCCCAGGCGAGCGTGGTGGTCCTTGAACCGGGC
  ACCATCGTAACCTCTGTCCAGGAAGTTGACTCCAAGACACAGCTTACCTGCTTTTTGAACAGCAGTGGCA
  TTGACATCGTTGGCCACCGCTGGATGAGAGGTGGCAAGGTACTGCAGGAAGACACGCTGCCCGATCTACA
  GATGAAGTACACGGTGGATGCAGATGACCGCTCTGGAGAATATTCCTGCATCTTCCTTCCTGAGCCTGTG
  GGCAGAGGCAACATCAATGTGGAGGGGCCACCCAGGATCAAGGTGGGAAAGAAATCGGAACACGCCAGTG
  AGGGAGAGTTTGTGAAGCTGATCTGCAAGTCTGAGGCGTCCCACCCTCCTGTGGATGAGTGGGTCTGGTT
  TAAGACCTCTGACACTGGGGACCAGACTATCTCCAACGGCACTGAGGCCAATAGCAAGTACGTCATTATA
  TCCACGCCTGAGCTGTCAGAGCTGATCATCAGCGACCTGGACATGAACGTGGACCCTGGCACCTACGTGT
  GCAATGCCACCAACTCCCAGGGCAGTGCTCGGGAGACCATCTCACTGCGTGTGCGCAGCCGCCTGGCAGC
  CCTCTGGCCCTTCCTGGGCATTGTGGCCGAGGTCCTGGTGTTGGTCACCATCATCTTCATCTACGAGAAG
  AGGCGGAAGCCGGACCAGACCCTGGACGAGGATGATCCTGGCGCCGCCCCACTGAAGGGCAGCGGGTCTC
  ACCTGAATGACAAGGACAAGAATGTGCGCCAGAGGAACGCCACCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001109882
• Insert Size: 1167 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001109882.1, NP_001103352.1
• RefSeq Size: 1681 bp
• RefSeq ORF: 1167 bp
• Locus ID: 25246
• UniProt ID: P26453
• Cytogenetics: 7q11
• Gene Summary: MRC OX-47 antigen that is upregulated on activated lymphocytes; member of the immunoglobulin superfamily [RGD, Feb 2006]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).