Calcr (NM_053816) Rat Untagged Clone

CAT#: RN200228

Calcr (untagged ORF) - Rat calcitonin receptor (Calcr), transcript variant 1, (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Calcr
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN200228 representing NM_053816
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTCCACGGAGATCCAGAATGAAAAGGCGGAACCTCCGAAAACCGAAGATGAGGTTCCTTCTCCTGA
  ACAGGTTCACCCTGCTGCTCCTGCTCCTAGTGAGCCCTACTCCAGTTCTTCAGGCTCCTACCAATCTCAC
  TGACTCCGGCCTTGATCAGGAGCCATTCCTGTACTTGGTTGGACGCAAGAAGCTTCTGGATGCTCAGTAC
  AAGTGCTATGACCGGATTCAGCAGTTGCCCCCTTATGAGGGAGAAGGTCCATATTGCAACCGAACTTGGG
  ATGGATGGATGTGCTGGGACGACACTCCAGCTGGTGTAATGTCCTATCAGCACTGCCCTGACTACTTTCC
  GGACTTTGACCCAACAGAAAAGGTTTCAAAATACTGCGATGAAAATGGAGAGTGGTTTAGACACCCTGAC
  AGCAACCGAACCTGGTCCAACTACACTCTGTGCAATGCCTTCACTCCTGACAAACTGCATAATGCATACG
  TTCTTTATTACCTAGCACTTGTGGGCCACTCCATGTCAATTGCTGCTCTGATTGCTTCCATGGGGATCTT
  CTTGTTTTTCAAGAACCTTAGCTGCCAGAGAGTGACTCTGCACAAGAACATGTTCCTCACTTATATCCTG
  AACTCTATCATTATCATCATCCACCTGGTTGAGGTTGTGCCCAATGGAGACCTGGTGCGACGGGATCCTA
  TGCATATTTTTCATCATAACACATATATGTGGACAATGCAGTGGGAACTGTCACCACCCTTACCCCTGAG
  TGCACACGAGGGAAAGATGGACCCTCATGACAGTGAAGTGATAAGTTGCAAGATTCTGCACTTTTTCCAT
  CAGTACATGATGGCTTGCAACTACTTCTGGATGCTGTGTGAGGGGATCTATCTTCACACTCTGATCGTCA
  TGGCTGTGTTCACCGAGGATCAACGTCTGCGCTGGTACTATTTACTTGGCTGGGGGTTCCCAATAGTGCC
  AACTATTATCCATGCCATTACTCGTGCCGTCTACTACAACGACAACTGTTGGCTGAGTACGGAGACCCAC
  TTGCTTTACATCATCCATGGACCCGTCATGGCGGCTCTGGTGGTCAACTTCTTCTTTCTGCTCAACATTG
  TCCGTGTGCTTGTGACCAAGATGAGGCAAACCCATGAAGCCGAGGCCTACATGTACCTGAAGGCTGTAAA
  GGCCACCATGGTCCTTGTGCCCCTGCTGGGGATCCAGTTTGTTGTGTTTCCCTGGAGGCCCTCCAACAAA
  GTGCTTGGGAAGATCTATGATTATCTCATGCACTCTCTGATTCATTTCCAGGGATTCTTTGTCGCGACTA
  TCTACTGCTTCTGTAACCATGAGGTGCAAGTGACCCTGAAGCGTCAGTGGGCGCAGTTCAAGATCCAGTG
  GAGCCATCGCTGGGGTAGGCGCCGCCGCCCCACCAACCGAGTGGTTAGCGCTCCTCGTGCTGTAGCCTTT
  GCAGAACCAGGTGGCCTCCCCATTTACATCTGCCATCAGGAACCAAGGAATCCTCCAGTCAGCAACAACG
  AAGGCGAAGAGGGTACTGAAATGATCCCCATGAATGTCATCCAGCAAGACTCATCCGCTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_053816
• Insert Size: 1602 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053816.2, NP_446268.2
• RefSeq Size: 3544 bp
• RefSeq ORF: 1602 bp
• Locus ID: 116506
• UniProt ID: P32214
• Cytogenetics: 4q13
• Gene Summary: receptor for calcitonin; acts via adenylate cyclase signaling [RGD, Feb 2006]
  Transcript Variant: This variant (1b), previously called 1, represents the longer variant and encodes the longer isoform (1b, previously called a).