GPRC6A (NM_001286355) Human Tagged ORF Clone

SKU RG239691

GPRC6A (tGFP-tagged) - Human G protein-coupled receptor, class C, group 6, member A (GPRC6A), transcript variant 3

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: GPRC6A
• Synonyms: bA86F4.3; GPCR
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239691 representing NM_001286355.; Blue=ORF Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGGCATTCTTAATTATACTAATTACCTGCTTTGTGATTATTCTTGCTACTTCACAGCCTTGCCAGACC; CCTGATGACTTTGTGGCTGCCACTTCTCCGGGACATATCATAATTGGAGGTTTGTTTGCTATTCATGAA; AAAATGTTGTCCTCAGAAGACTCTCCCAGACGACCACAAATCCAGGAGTGTGTTGGCTTTGAAATATCA; GTTTTTCTTCAAACTCTTGCCATGATACACAGCATTGAGATGATCAACAATTCAACACTCTTACCTGGA; GTCAAACTGGGGTATGAAATCTATGACACTTGTACAGAAGTCACAGTGGCAATGGCAGCCACTCTGAGG; TTTCTTTCTAAATTCAACTGCTCCAGAGAAACTGTGGAGTTTAAGTGTGACTATTCCAGCTACATGCCA; AGAGTTAAGGCTGTCATAGGTTCTGGGTACTCAGAAATAACTATGGCTGTCTCCAGGATGTTGAATTTA; CAGCTCATGCCACAGGTGGGTTATGAATCAACTGCAGAAATCCTGAGTGACAAAATTCGCTTTCCTTCA; TTTTTACGGACTGTGCCCAGTGACTTCCATCAAATTAAAGCAATGGCTCACCTGATTCAGAAATCTGGT; TGGAACTGGATTGGCATCATAACCACAGATGATGACTATGGACGATTGGCTCTTAACACTTTTATAATT; CAGGCTGAAGCAAATAACGTGTGCATAGCCTTCAAAGAGGTTCTTCCAGCCTTTCTTTCAGATAATACC; ATTGAAGTCAGAATCAATCGGACACTGAAGAAAATCATTTTAGAAGCCCAGGTTAATGTCATTGTGGTA; TTTCTGAGGCAATTCCATGTTTTTGATCTCTTCAATAAAGCCATTGAAATGAATATAAATAAGATGTGG; ATTGCTAGTGATAATTGGTCAACTGCCACCAAGATTACCACCATTCCTAATGTTAAAAAGATTGGCAAA; GTTGTAGGGTTTGCCTTTAGAAGAGGGAATATATCCTCTTTCCATTCCTTTCTTCAAAATCTGCACTTG; CTTCCCAGTGACAGTCACAAACTCTTACATGAATATGCCATGCATTTATCTGCCTGCGCATATGTCAAG; GACACTGATTTGAGTCAATGCATATTCAATCATTCTCAAAGGACTTTGGCCTACAAGGCTAACAAGGCT; ATAGAAAGGAACTTCGTCATGAGAAATGACTTCCTCTGGGACTATGCTGAGCCAGGACTCATTCATAGT; ATTCAGCTTGCAGTGTTTGCCCTTGGTTATGCCATTCGGGATCTGTGTCAAGCTCGTGACTGTCAGAAC; CCCAACGCCTTTCAACCATGGGAGCAAATTCAATCTAAATGCTCCAAGGAATGCAGTCCTGGGCAAATG; AAGAAAACTACAAGAAGTCAACACATCTGTTGCTATGAATGTCAGAACTGTCCTGAAAATCATTACACT; AATCAGACAGATATGCCTCACTGCCTTTTATGCAACAACAAAACTCACTGGGCCCCTGTTAGGAGCACT; ATGTGCTTTGAAAAGGAAGTGGAATATCTCAACTGGAATGACTCCTTGGCCATCCTACTCCTGATTCTC; TCCCTACTGGGAATCATATTTGTTCTGGTTGTTGGCATAATATTTACAAGAAACCTGAACACACCTGTT; GTGAAATCATCCGGGGGATTAAGAGTCTGCTATGTGATCCTTCTCTGTCATTTCCTCAATTTTGCCAGC; ACGAGCTTTTTCATTGGAGAACCACAAGACTTCACATGTAAAACCAGGCAGACAATGTTTGGAGTGAGC; TTTACTCTTTGCATCTCCTGCATTTTGACGAAGTCTCTGAAAATTTTGCTAGCCTTCAGCTTTGATCCC; AAATTACAGAAATTTCTGAAGTGCCTCTATAGACCGATCCTTATTATCTTCACTTGCACGGGCATCCAG; GTTGTCATTTGCACACTCTGGCTAATCTTTGCAGCACCTACTGTAGAGGTGAATGTCTCCTTGCCCAGA; GTCATCATCCTGGAGTGTGAGGAGGGATCCATACTTGCATTTGGCACCATGCTGGGCTACATTGCCATC; CTGGCCTTCATTTGCTTCATATTTGCTTTCAAAGGCAAATATGAGAATTACAATGAAGCCAAATTCATT; ACATTTGGCATGCTCATTTACTTCATAGCTTGGATCACATTCATCCCTATCTATGCTACCACATTTGGC; AAATATGTACCAGCTGTGGAGATTATTGTCATATTAATATCTAACTATGGAATCCTGTATTGCACATTC; ATCCCCAAATGCTATGTTATTATTTGTAAGCAAGAGATTAACACAAAGTCTGCCTTTCTCAAGATGATC; TACAGTTATTCTTCCCATAGTGTGAGCAGCATTGCCCTGAGTCCTGCTTCACTGGACTCCATGAGCGGC; AATGTCACAATGACCAATCCCAGCTCTAGTGGCAAGTCTGCAACCTGGCAGAAAAGCAAAGATCTTCAG; GCACAAGCATTTGCACACATATGCAGGGAAAATGCCACAAGTGTATCTAAAACTTTGCCTCGAAAAAGA; ATGTCAAGTATA; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC;
  Protein Sequence

  >Peptide sequence encoded by RG239691; Blue=ORF Red=Cloning site Green=Tag(s); ; MAFLIILITCFVIILATSQPCQTPDDFVAATSPGHIIIGGLFAIHEKMLSSEDSPRRPQIQECVGFEIS; VFLQTLAMIHSIEMINNSTLLPGVKLGYEIYDTCTEVTVAMAATLRFLSKFNCSRETVEFKCDYSSYMP; RVKAVIGSGYSEITMAVSRMLNLQLMPQVGYESTAEILSDKIRFPSFLRTVPSDFHQIKAMAHLIQKSG; WNWIGIITTDDDYGRLALNTFIIQAEANNVCIAFKEVLPAFLSDNTIEVRINRTLKKIILEAQVNVIVV; FLRQFHVFDLFNKAIEMNINKMWIASDNWSTATKITTIPNVKKIGKVVGFAFRRGNISSFHSFLQNLHL; LPSDSHKLLHEYAMHLSACAYVKDTDLSQCIFNHSQRTLAYKANKAIERNFVMRNDFLWDYAEPGLIHS; IQLAVFALGYAIRDLCQARDCQNPNAFQPWEQIQSKCSKECSPGQMKKTTRSQHICCYECQNCPENHYT; NQTDMPHCLLCNNKTHWAPVRSTMCFEKEVEYLNWNDSLAILLLILSLLGIIFVLVVGIIFTRNLNTPV; VKSSGGLRVCYVILLCHFLNFASTSFFIGEPQDFTCKTRQTMFGVSFTLCISCILTKSLKILLAFSFDP; KLQKFLKCLYRPILIIFTCTGIQVVICTLWLIFAAPTVEVNVSLPRVIILECEEGSILAFGTMLGYIAI; LAFICFIFAFKGKYENYNEAKFITFGMLIYFIAWITFIPIYATTFGKYVPAVEIIVILISNYGILYCTF; IPKCYVIICKQEINTKSAFLKMIYSYSSHSVSSIALSPASLDSMSGNVTMTNPSSSGKSATWQKSKDLQ; AQAFAHICRENATSVSKTLPRKRMSSI; TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV; MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED; SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA; FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV;
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001286355
• ORF Size: 2603 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001286355.1, NP_001273284.1
• RefSeq Size: 2669 bp
• RefSeq ORF: 2568 bp
• Locus ID: 222545
• UniProt ID: Q5T6X5
• Cytogenetics: 6q22.1
• Protein Families: Druggable Genome, Transmembrane
• MW: 97 kDa
• Summary: Members of family C of the G protein-coupled receptor (GPCR) superfamily, such as GPRC6A, are characterized by an evolutionarily conserved amino acid-sensing motif linked to an intramembranous 7-transmembrane loop region. Several members of GPCR family C, including GPRC6A, also have a long N-terminal domain (summary by Pi et al., 2005 [PubMed 16199532]).[supplied by OMIM, Nov 2010]