LASS3 (CERS3) (NM_001290341) Human Tagged ORF Clone

SKU RG237945

CERS3 (tGFP-tagged) - Human ceramide synthase 3 (CERS3), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: LASS3
• Synonyms: ARCI9; LASS3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG237945 representing NM_001290341.; Blue=ORF Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGCTTCACACCACTGCCAGAAGAGCTGCCAGAATGTTTTGGACGTTTAAAGAATGGTTCTGGTTGGAA; AGATTCTGGCTTCCTCCAACAATAAAGTGGTCAGATCTTGAGGATCACGATGGACTCGTCTTTGTAAAA; CCTTCTCATTTATACGTGACAATTCCATATGCTTTTCTCTTGCTGATTATCAGACGTGTATTTGAAAAA; TTTGTTGCTTCACCTCTAGCAAAATCATTTGGCATTAAAGAGACAGTTCGAAAGGTTACACCAAATACT; GTCTTAGAGAATTTTTTCAAACATTCCACAAGGCAACCATTGCAAACTGATATTTATGGACTGGCAAAG; AAGTGTAACTTGACGGAGCGCCAGGTGGAAAGATGGTTTAGGAGTCGGCGGAATCAAGAGAGGCCTTCC; AGGCTGAAGAAATTCCAGGAAGCTTGCTGGAGATTTGCATTTTACTTAATGATCACTGTTGCTGGAATT; GCGTTTCTTTATGATAAACCTTGGCTATATGACTTATGGGAGGTTTGGAATGGCTATCCCAAACAGCCC; CTGCTGCCATCCCAGTACTGGTACTACATTTTAGAAATGAGTTTTTATTGGTCTCTGTTATTTAGACTT; GGCTTTGATGTCAAGAGAAAGGATTTTCTAGCTCATATCATCCACCACCTGGCTGCTATTAGTCTGATG; AGCTTCTCTTGGTGTGCTAATTATATTCGCAGTGGGACCCTCGTGATGATTGTACACGATGTGGCTGAC; ATTTGGCTGGAGTCTGCTAAGATGTTTTCTTATGCTGGATGGACGCAGACCTGTAACACCCTGTTTTTC; ATCTTCTCCACCATATTTTTCATCAGCCGCCTCATTGTTTTTCCTTTCTGGATTTTATATTGCACGCTG; ATCTTGCCTATGTATCACCTCGAGCCTTTCTTTTCATACATCTTCCTCAACCTACAGCTCATGATCTTG; CAGGTCCTTCACCTTTACTGGGGTTATTACATCTTGAAGATGCTCAACAGATGTATATTCATGAAGAGC; ATCCAGGATGTGAGGAGTGATGACGAGGATTATGAAGAGGAAGAGGAAGAGGAAGAAGAAGAGGCTACC; AAAGGCAAAGAGATGGATTGTTTAAAGAACGGCCTCAGGGCTGAGAGGCACCTCATTCCCAATGGCCAG; CATGGCCAT; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC;
  Protein Sequence

  >Peptide sequence encoded by RG237945; Blue=ORF Red=Cloning site Green=Tag(s); ; MLHTTARRAARMFWTFKEWFWLERFWLPPTIKWSDLEDHDGLVFVKPSHLYVTIPYAFLLLIIRRVFEK; FVASPLAKSFGIKETVRKVTPNTVLENFFKHSTRQPLQTDIYGLAKKCNLTERQVERWFRSRRNQERPS; RLKKFQEACWRFAFYLMITVAGIAFLYDKPWLYDLWEVWNGYPKQPLLPSQYWYYILEMSFYWSLLFRL; GFDVKRKDFLAHIIHHLAAISLMSFSWCANYIRSGTLVMIVHDVADIWLESAKMFSYAGWTQTCNTLFF; IFSTIFFISRLIVFPFWILYCTLILPMYHLEPFFSYIFLNLQLMILQVLHLYWGYYILKMLNRCIFMKS; IQDVRSDDEDYEEEEEEEEEEATKGKEMDCLKNGLRAERHLIPNGQHGH; TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV; MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED; SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA; FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV;
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001290341
• ORF Size: 1200 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001290341.2, NP_001277270.1
• RefSeq Size: 4089 bp
• RefSeq ORF: 1185 bp
• Locus ID: 204219
• UniProt ID: Q8IU89
• Cytogenetics: 15q26.3
• Protein Families: Transcription Factors, Transmembrane
• MW: 48 kDa
• Summary: This gene is a member of the ceramide synthase family of genes. The ceramide synthase enzymes regulate sphingolipid synthesis by catalyzing the formation of ceramides from sphingoid base and acyl-coA substrates. This family member is involved in the synthesis of ceramides with ultra-long-chain acyl moieties (ULC-Cers), important to the epidermis in its role in creating a protective barrier from the environment. The protein encoded by this gene has also been implicated in modification of the lipid structures required for spermatogenesis. Mutations in this gene have been associated with male fertility defects, and epidermal defects, including ichthyosis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015]