PHF7 (NM_001278221) Human Tagged ORF Clone

CAT#: RG237589

PHF7 (tGFP-tagged) - Human PHD finger protein 7 (PHF7), transcript variant 2

Product Images

Circular map for RG237589

Specifications

Product Data

• Tag: TurboGFP
• Symbol: PHF7
• Synonyms: HSPC045; HSPC226; NYD-SP6
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG237589 representing NM_001278221.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAAGACTGTAAAAGAAAAGAAGGAATGCCAGAGATTGAGAAAATCTGCCAAGACTAGGAGGGTAACC
  CAGAGGAAACCGTCTTCAGGGCCTGTTTGCTGGCTATGCCTTCGAGAACCTGGGGATCCCGAAAAATTA
  GGGGAATTTCTTCAGAAAGACAATATCAGCGTGCATTATTTCTGTCTTATCTTATCTAGTAAGCTGCCT
  CAGAGGGGCCAGTCCAACAGAGGCTTCCATGGATTTCTGCCTGAAGACATCAAAAAGGAGGCAGCCCGG
  GCTTCTAGGAAGATCTGCTTTGTGTGCAAGAAAAAGGGAGCTGCTATCAACTGCCAGAAGGATCAGTGC
  CTCAGAAACTTCCATCTGCCTTGTGGCCAAGAAAGGGGTTGCCTTTCACAATTTTTTGGAGAGTACAAA
  TCATTTTGTGACAAACATCGCCCAACACAGAACATCCAACATGGGCATGTGGGGGAGGAAAGCTGCATC
  TTATGTTGTGAAGACTTATCCCAACAGAGTGTTGAGAACATCCAGAGCCCGTGTTGTAGTCAAGCCATC
  TACCACCGCAAGTGCATACAGAAATATGCCCACACATCAGCAAAGCATTTCTTCAAATGTCCACAGTGT
  AACAATCGAAAAGAGTTTCCTCAAGAAATGCTGAGAATGGGAATTCATATTCCAGACAGGAGGTGGTGC
  CTCATTCTGTGTGCTACATGCGGATCCCACGGAACCCACAGGGACTGCTCCTCTCTTAGATCTAACAGT
  AAGAAATGGGAGTGTGAGGAGTGTTCACCTGCTGCAGCCACAGACTACATACCTGAAAACTCAGGGGAC
  ATCCCTTGCTGCAGCAGCACCTTCCACCCTGAGGAACATTTCTGCAGAGACAACACCTTGGAAGAGAAT
  CCGGGCCTTTCTTGGACTGATTGGCCAGAACCTTCCTTATTAGAAAAGCCAGAGTCCTCTCGTGGCAGG
  AGGAGCTACTCCTGGAGGTCCAAGGGTGTCAGAATCACTAACAGCTGCAAAAAATCCAAG
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG237589
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MKTVKEKKECQRLRKSAKTRRVTQRKPSSGPVCWLCLREPGDPEKLGEFLQKDNISVHYFCLILSSKLP
  QRGQSNRGFHGFLPEDIKKEAARASRKICFVCKKKGAAINCQKDQCLRNFHLPCGQERGCLSQFFGEYK
  SFCDKHRPTQNIQHGHVGEESCILCCEDLSQQSVENIQSPCCSQAIYHRKCIQKYAHTSAKHFFKCPQC
  NNRKEFPQEMLRMGIHIPDRRWCLILCATCGSHGTHRDCSSLRSNSKKWECEECSPAAATDYIPENSGD
  IPCCSSTFHPEEHFCRDNTLEENPGLSWTDWPEPSLLEKPESSRGRRSYSWRSKGVRITNSCKKSK
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001278221
• ORF Size: 1026 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).

Reference Data

• RefSeq: NM_001278221.3
• RefSeq Size: 1436 bp
• RefSeq ORF: 1029 bp
• Locus ID: 51533
• UniProt ID: Q9BWX1
• Cytogenetics: 3p21.1
• Protein Families: Druggable Genome, Transcription Factors
• MW: 39.3 kDa
• Gene Summary: Spermatogenesis is a complex process regulated by extracellular and intracellular factors as well as cellular interactions among interstitial cells of the testis, Sertoli cells, and germ cells. This gene is expressed in the testis in Sertoli cells but not germ cells. The protein encoded by this gene contains plant homeodomain (PHD) finger domains, also known as leukemia associated protein (LAP) domains, believed to be involved in transcriptional regulation. The protein, which localizes to the nucleus of transfected cells, has been implicated in the transcriptional regulation of spermatogenesis. Alternate splicing results in multiple transcript variants of this gene. [provided by RefSeq, May 2013]