SNX17 (NM_001267059) Human Tagged ORF Clone

CAT#: RG234157

SNX17 (tGFP-tagged) - Homo sapiens sorting nexin 17 (SNX17), transcript variant 2

Product Images

Circular map for RG234157

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SNX17
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG234157 representing NM_001267059
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACTTTTCCATTCCCGAAACCGAGTCCCGCAGCGGGGACAGCGGCGGCTCCGCCTACGTGGCCTATA
  ACATTCACGTGAATGGAGTCCTGCACTGTCGGCTTCGGAAGGAGTATGGGGCCAATGTGCTTCCTGCATT
  CCCCCCAAAGAAGCTTTTCTCTCTGACTCCTGCTGAGGTAGAACAGAGGAGAGAGCAGTTAGAGAAGTAC
  ATGCAAGCTGTTCGGCAAGACCCATTGCTTGGGAGCAGCGAGACTTTCAACAGTTTCCTGCGTCGGGCAC
  AACAGGAGACACAGCAGGTCCCCACAGAGGAAGTGTCCTTGGAAGTGCTGCTCAGCAACGGGCAGAAAGT
  TCTGGTCAACGTGCTAACTTCAGATCAGACTGAGGATGTCCTGGAGGCTGTAGCTGCAAAGCTGGATCTT
  CCAGATGACTTGATTGGATACTTTAGTCTATTCTTAGTTCGAGAAAAAGAGGATGGAGCCTTTTCTTTTG
  TACGGAAGTTGCAAGAGTTTGAGCTGCCTTATGTGTCTGTCACCAGCCTTCGGAGTCAAGAGTATAAGAT
  TGTGCTAAGGAAGAGTTATTGGGACTCTGCCTATGATGACGATGTCATGGAGAACCGGGTTGGCCTGAAC
  CTGCTTTATGCTCAGACGGTATCAGATATTGAGCGTGGGTGGATCTTGGTCACCAAGGAACAGCACCGGC
  AACTCAAATCTCTGCAAGAGAAAGTCTCCAAGAAGGAGTTCCTGAGACTGGCCCAGACGCTGCGGCACTA
  TGGCTACTTGCGCTTTGATGCCTGTGTGGCTGACTTCCCAGAAAAGGACTGTCCTGTGGTGGTGAGCGCG
  GGCAACAGTGAGCTCAGCCTGCAGCTCCGCCTGCCTGGCCAGCAACTCCGAGAAGGCTCCTTCCGGGTCA
  CCCGCATGCGATGCTGGCGGGTCACCTCCTCTGTACCATTGCCCAGTGGAAGCACGAGCAGCCCAGGCCG
  GGGCCGGGGTGAGGTGCGCCTGGAACTGGCTTTTGAATACCTCATGAGCAAGGACCGGCTACAGTGGGTC
  ACCATCACTAGCCCCCAGGCTATCATGATGAGCATCTGCTTGCAGTCCATGGTTGATGAACTGATGGTGA
  AGAAATCTGGCGGCAGTATCAGGAAGATGCTGCGCCGGCGGGTGGGGGGTACTCTGAGACGCTCAGACAG
  CCAGCAAGCAGTGAAGTCCCCACCACTGCTTGAGTCACCTGATGCCACCCGGGAGTCTATGGTCAAACTC
  TCAAGTAAGCTGAGTGCCGTGAGCTTGCGGGGAATTGGCAGTCCCAGCACAGATGCCAGTGCCAGTGATG
  TCCACGGCAATTTCGCCTTCGAGGGCATTGGAGATGAGGATCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG234157 representing NM_001267059
  Red=Cloning site Green=Tags(s)
  
  MHFSIPETESRSGDSGGSAYVAYNIHVNGVLHCRLRKEYGANVLPAFPPKKLFSLTPAEVEQRREQLEKY
  MQAVRQDPLLGSSETFNSFLRRAQQETQQVPTEEVSLEVLLSNGQKVLVNVLTSDQTEDVLEAVAAKLDL
  PDDLIGYFSLFLVREKEDGAFSFVRKLQEFELPYVSVTSLRSQEYKIVLRKSYWDSAYDDDVMENRVGLN
  LLYAQTVSDIERGWILVTKEQHRQLKSLQEKVSKKEFLRLAQTLRHYGYLRFDACVADFPEKDCPVVVSA
  GNSELSLQLRLPGQQLREGSFRVTRMRCWRVTSSVPLPSGSTSSPGRGRGEVRLELAFEYLMSKDRLQWV
  TITSPQAIMMSICLQSMVDELMVKKSGGSIRKMLRRRVGGTLRRSDSQQAVKSPPLLESPDATRESMVKL
  SSKLSAVSLRGIGSPSTDASASDVHGNFAFEGIGDEDL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001267059
• ORF Size: 1374 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001267059.2
• RefSeq Size: 2439 bp
• RefSeq ORF: 1377 bp
• Locus ID: 9784
• Cytogenetics: 2p23.3
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This protein does not contain a coiled coil region, like some family members, but contains a B41 domain. This protein interacts with the cytoplasmic domain of P-selectin, and may function in the intracellular trafficking of P-selectin. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2012]