CD166 (ALCAM) (NM_001243280) Human Tagged ORF Clone

CAT#: RG232980

ALCAM (tGFP-tagged) - Homo sapiens activated leukocyte cell adhesion molecule (ALCAM), transcript variant 2

Product Images

Circular map for RG232980

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CD166
• Synonyms: CD166; MEMD
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG232980 representing NM_001243280.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGAATCCAAGGGGGCCAGTTCCTGCCGTCTGCTCTTCTGCCTCTTGATCTCCGCCACCGTCTTCAGG
  CCAGGCCTTGGATGGTATACTGTAAATTCAGCATATGGAGATACCATTATCATACCTTGCCGACTTGAC
  GTACCTCAGAATCTCATGTTTGGCAAATGGAAATATGAAAAGCCCGATGGCTCCCCAGTATTTATTGCC
  TTCAGATCCTCTACAAAGAAAAGTGTGCAGTACGACGATGTACCAGAATACAAAGACAGATTGAACCTC
  TCAGAAAACTACACTTTGTCTATCAGTAATGCAAGGATCAGTGATGAAAAGAGATTTGTGTGCATGCTA
  GTAACTGAGGACAACGTGTTTGAGGCACCTACAATAGTCAAGGTGTTCAAGCAACCATCTAAACCTGAA
  ATTGTAAGCAAAGCACTGTTTCTCGAAACAGAGCAGCTAAAAAAGTTGGGTGACTGCATTTCAGAAGAC
  AGTTATCCAGATGGCAATATCACATGGTACAGGAATGGAAAAGTGCTACATCCCCTTGAAGGAGCGGTG
  GTCATAATTTTTAAAAAGGAAATGGACCCAGTGACTCAGCTCTATACCATGACTTCCACCCTGGAGTAC
  AAGACAACCAAGGCTGACATACAAATGCCATTCACCTGCTCGGTGACATATTATGGACCATCTGGCCAG
  AAAACAATTCATTCTGAACAGGCAGTATTTGATATTTACTATCCTACAGAGCAGGTGACAATACAAGTG
  CTGCCACCAAAAAATGCCATCAAAGAAGGGGATAACATCACTCTTAAATGCTTAGGGAATGGCAACCCT
  CCCCCAGAGGAATTTTTGTTTTACTTACCAGGACAGCCCGAAGGAATAAGAAGCTCAAATACTTACACA
  CTGACGGATGTGAGGCGCAATGCAACAGGAGACTACAAGTGTTCCCTGATAGACAAAAAAAGCATGATT
  GCTTCAACAGCCATCACAGTTCACTATTTGGATTTGTCCTTAAACCCAAGTGGAGAAGTGACTAGACAG
  ATTGGTGATGCCCTACCCGTGTCATGCACAATATCTGCTAGCAGGAATGCAACTGTGGTATGGATGAAA
  GATAACATCAGGCTTCGATCTAGCCCGTCATTTTCTAGTCTTCATTATCAGGATGCTGGAAACTATGTC
  TGCGAAACTGCTCTGCAGGAGGTTGAAGGACTAAAGAAAAGAGAGTCATTGACTCTCATTGTAGAAGGC
  AAACCTCAAATAAAAATGACAAAGAAAACTGATCCCAGTGGACTATCTAAAACAATAATCTGCCATGTG
  GAAGGTTTTCCAAAGCCAGCCATTCAATGGACAATTACTGGCAGTGGAAGCGTCATAAACCAAACAGAG
  GAATCTCCTTATATTAATGGCAGGTATTATAGTAAAATTATCATTTCCCCTGAAGAGAATGTTACATTA
  ACTTGCACAGCAGAAAACCAACTGGAGAGAACAGTAAACTCCTTGAATGTCTCTGCTAATGAAAACAGA
  GAAAAGGTGAATGACCAGGCAAAACTAATTGTGGGAATCGTTGTTGGTCTCCTCCTTGCTGCCCTTGTT
  GCTGGTGTCGTCTACTGGCTGTACATGAAGAAGTCAAAGACTGCATCAAAACATGTAAACAAGGACCTC
  GGTAATATGGAAGAAAACAAAAAGTTAGAAGAAAACAATCACAAAACTGAAGCC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG232980
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MESKGASSCRLLFCLLISATVFRPGLGWYTVNSAYGDTIIIPCRLDVPQNLMFGKWKYEKPDGSPVFIA
  FRSSTKKSVQYDDVPEYKDRLNLSENYTLSISNARISDEKRFVCMLVTEDNVFEAPTIVKVFKQPSKPE
  IVSKALFLETEQLKKLGDCISEDSYPDGNITWYRNGKVLHPLEGAVVIIFKKEMDPVTQLYTMTSTLEY
  KTTKADIQMPFTCSVTYYGPSGQKTIHSEQAVFDIYYPTEQVTIQVLPPKNAIKEGDNITLKCLGNGNP
  PPEEFLFYLPGQPEGIRSSNTYTLTDVRRNATGDYKCSLIDKKSMIASTAITVHYLDLSLNPSGEVTRQ
  IGDALPVSCTISASRNATVVWMKDNIRLRSSPSFSSLHYQDAGNYVCETALQEVEGLKKRESLTLIVEG
  KPQIKMTKKTDPSGLSKTIICHVEGFPKPAIQWTITGSGSVINQTEESPYINGRYYSKIIISPEENVTL
  TCTAENQLERTVNSLNVSANENREKVNDQAKLIVGIVVGLLLAALVAGVVYWLYMKKSKTASKHVNKDL
  GNMEENKKLEENNHKTEA
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001243280
• ORF Size: 1710 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001243280.1, NP_001230209.1
• RefSeq Size: 4884 bp
• RefSeq ORF: 1713 bp
• Locus ID: 214
• UniProt ID: Q13740
• Cytogenetics: 3q13.11
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs)
• MW: 63.7 kDa
• Gene Summary: This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Aug 2011]