MIF4GD (NM_001242498) Human Tagged ORF Clone

CAT#: RG232321

MIF4GD (tGFP-tagged) - Homo sapiens MIF4G domain containing (MIF4GD), transcript variant 1

Product Images

Circular map for RG232321

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MIF4GD
• Synonyms: AD023; MIFD; SLIP1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG232321 representing NM_001242498
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGAGCCCAGTAGAGAGGAGTATAAAATCCAGTCCTTTGATGCAGAGACCCAGCAGCTGCTGAAGA
  CAGCACTCAAAGCCCCGTCTCTTGAATGCACAGTTGCCTGCTTTGAGACAGAAGATGGAGAATATTCCGT
  TTGCCAGAGGAGCTATAGTAATTGCTCTCGCCTGATGCCTTCCCGCTGTAACACGCAGTACAGAGATCCG
  GGTGCTGTGGACTTGGAGAAAGTGGCCAATGTGATTGTGGACCATTCTCTGCAGGACTGTGTGTTCAGCA
  AGGAAGCAGGACGCATGTGCTACGCCATCATTCAGGCAGAGAGTAAACAAGCAGGCCAGAGTGTCTTCCG
  ACGTGGACTCCTCAACCGGCTGCAGCAGGAGTACCAGGCTCGGGAGCAGCTGCGAGCACGCTCCCTGCAG
  GGCTGGGTCTGCTATGTCACCTTTATCTGCAACATCTTTGACTACCTGAGGGTGAACAACATGCCCATGA
  TGGCCCTGGTGAACCCTGTCTATGACTGCCTCTTCCGGCTGGCCCAGCCAGACAGTTTGAGCAAGGAGGA
  GGAGGTGGACTGTTTGGTGCTGCAGCTGCACCGGGTTGGGGAGCAGCTGGAGAAAATGAATGGGCAGCGC
  ATGGATGAGCTCTTTGTGCTGATCCGGGATGGCTTCCTGCTCCCAACTGGCCTCAGCTCCCTGGCCCAGC
  TGCTGCTGCTGGAGATCATTGAGTTCCGGGCGGCCGGCTGGAAGACAACGCCAGCTGCCCACAAGTATTA
  CTACAGCGAAGTCTCCGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG232321 representing NM_001242498
  Red=Cloning site Green=Tags(s)
  
  MGEPSREEYKIQSFDAETQQLLKTALKAPSLECTVACFETEDGEYSVCQRSYSNCSRLMPSRCNTQYRDP
  GAVDLEKVANVIVDHSLQDCVFSKEAGRMCYAIIQAESKQAGQSVFRRGLLNRLQQEYQAREQLRARSLQ
  GWVCYVTFICNIFDYLRVNNMPMMALVNPVYDCLFRLAQPDSLSKEEEVDCLVLQLHRVGEQLEKMNGQR
  MDELFVLIRDGFLLPTGLSSLAQLLLLEIIEFRAAGWKTTPAAHKYYYSEVSD
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001242498
• ORF Size: 789 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001242498.1, NP_001229427.1
• RefSeq Size: 1462 bp
• RefSeq ORF: 792 bp
• Locus ID: 57409
• UniProt ID: A9UHW6
• Cytogenetics: 17q25.1
• Gene Summary: This gene encodes a protein which interacts with the N-terminus of the stem-loop binding protein (SLBP) and the 3' end of histone mRNA. This interaction facilitates the activation of histone mRNA translation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]