PDE4D (NM_001197218) Human Tagged ORF Clone

CAT#: RG231398

PDE4D (tGFP-tagged) - Human phosphodiesterase 4D, cAMP-specific (PDE4D), transcript variant 4

Product Images

Circular map for RG231398

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PDE4D
• Synonyms: ACRDYS2; DPDE3; HSPDE4D; PDE4DN2; PDE43; STRK1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG231398 representing NM_001197218
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCAGCAGACAAGCCCGGACACTTTAACAGTACCTGAAGTGGATAATCCGCATTGTCCAAACCCGT
  GGCTGAACGAAGACCTTGTGAAATCCTTGCGAGAAAACCTGTTGCAGCATGAGAAGTCCAAGACAGCGAG
  GAAATCGGTTTCTCCCAAGCTCTCTCCAGTGATCTCTCCGAGAAATTCCCCCAGGCTTCTGCGCAGAATG
  CTTCTCAGCAGCAACATCCCCAAACAGCGGCGTTTCACGGTGGCACATACATGTTTTGATGTGGACAATG
  GCACATCTGCGGGACGGAGTCCCTTGGATCCCATGACCAGCCCAGGATCCGGGCTAATTCTCCAAGCAAA
  TTTTGTCCACAGTCAACGACGGGAGTCCTTCCTGTATCGATCCGACAGCGATTATGACCTCTCTCCAAAG
  TCTATGTCCCGGAACTCCTCCATTGCCAGTGATATACACGGAGATGACTTGATTGTGACTCCATTTGCTC
  AGGTCTTGGCCAGTCTGCGAACTGTACGAAACAACTTTGCTGCATTAACTAATTTGCAAGATCGAGCACC
  TAGCAAAAGATCACCCATGTGCAACCAACCATCCATCAACAAAGCCACCATAACAGAGGAGGCCTACCAG
  AAACTGGCCAGCGAGACCCTGGAGGAGCTGGACTGGTGTCTGGACCAGCTAGAGACCCTACAGACCAGGC
  ACTCCGTCAGTGAGATGGCCTCCAACAAGTTTAAAAGGATGCTTAATCGGGAGCTCACCCATCTCTCTGA
  AATGAGTCGGTCTGGAAATCAAGTGTCAGAGTTTATATCAAACACATTCTTAGATAAGCAACATGAAGTG
  GAAATTCCTTCTCCAACTCAGAAGGAAAAGGAGAAAAAGAAAAGACCAATGTCTCAGATCAGTGGAGTCA
  AGAAATTGATGCACAGCTCTAGTCTGACTAATTCAAGTATCCCAAGGTTTGGAGTTAAAACTGAACAAGA
  AGATGTCCTTGCCAAGGAACTAGAAGATGTGAACAAATGGGGTCTTCATGTTTTCAGAATAGCAGAGTTG
  TCTGGTAACCGGCCCTTGACTGTTATCATGCACACCATTTTTCAGGAACGGGATTTATTAAAAACATTTA
  AAATTCCAGTAGATACTTTAATTACATATCTTATGACTCTCGAAGACCATTACCATGCTGATGTGGCCTA
  TCACAACAATATCCATGCTGCAGATGTTGTCCAGTCTACTCATGTGCTATTATCTACACCTGCTTTGGAG
  GCTGTGTTTACAGATTTGGAGATTCTTGCAGCAATTTTTGCCAGTGCAATACATGATGTAGATCATCCTG
  GTGTGTCCAATCAATTTCTGATCAATACAAACTCTGAACTTGCCTTGATGTACAATGATTCCTCAGTCTT
  AGAGAACCATCATTTGGCTGTGGGCTTTAAATTGCTTCAGGAAGAAAACTGTGACATTTTCCAGAATTTG
  ACCAAAAAACAAAGACAATCTTTAAGGAAAATGGTCATTGACATCGTACTTGCAACAGATATGTCAAAAC
  ACATGAATCTACTGGCTGATTTGAAGACTATGGTTGAAACTAAGAAAGTGACAAGCTCTGGAGTTCTTCT
  TCTTGATAATTATTCCGATAGGATTCAGGTTCTTCAGAATATGGTGCACTGTGCAGATCTGAGCAACCCA
  ACAAAGCCTCTCCAGCTGTACCGCCAGTGGACGGACCGGATAATGGAGGAGTTCTTCCGCCAAGGAGACC
  GAGAGAGGGAACGTGGCATGGAGATAAGCCCCATGTGTGACAAGCACAATGCTTCCGTGGAAAAATCACA
  GGTGGGCTTCATAGACTATATTGTTCATCCCCTCTGGGAGACATGGGCAGACCTCGTCCACCCTGACGCC
  CAGGATATTTTGGACACTTTGGAGGACAATCGTGAATGGTACCAGAGCACAATCCCTCAGAGCCCCTCTC
  CTGCACCTGATGACCCAGAGGAGGGCCGGCAGGGTCAAACTGAGAAATTCCAGTTTGAACTAACTTTAGA
  GGAAGATGGTGAGTCAGACACGGAAAAGGACAGTGGCAGTCAAGTGGAAGAAGACACTAGCTGCAGTGAC
  TCCAAGACTCTTTGTACTCAAGACTCAGAGTCTACTGAAATTCCCCTTGATGAACAGGTTGAAGAGGAGG
  CAGTAGGGGAAGAAGAGGAAAGCCAGCCTGAAGCCTGTGTCATAGATGATCGTTCTCCTGACACG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG231398 representing NM_001197218
  Red=Cloning site Green=Tags(s)
  
  MAQQTSPDTLTVPEVDNPHCPNPWLNEDLVKSLRENLLQHEKSKTARKSVSPKLSPVISPRNSPRLLRRM
  LLSSNIPKQRRFTVAHTCFDVDNGTSAGRSPLDPMTSPGSGLILQANFVHSQRRESFLYRSDSDYDLSPK
  SMSRNSSIASDIHGDDLIVTPFAQVLASLRTVRNNFAALTNLQDRAPSKRSPMCNQPSINKATITEEAYQ
  KLASETLEELDWCLDQLETLQTRHSVSEMASNKFKRMLNRELTHLSEMSRSGNQVSEFISNTFLDKQHEV
  EIPSPTQKEKEKKKRPMSQISGVKKLMHSSSLTNSSIPRFGVKTEQEDVLAKELEDVNKWGLHVFRIAEL
  SGNRPLTVIMHTIFQERDLLKTFKIPVDTLITYLMTLEDHYHADVAYHNNIHAADVVQSTHVLLSTPALE
  AVFTDLEILAAIFASAIHDVDHPGVSNQFLINTNSELALMYNDSSVLENHHLAVGFKLLQEENCDIFQNL
  TKKQRQSLRKMVIDIVLATDMSKHMNLLADLKTMVETKKVTSSGVLLLDNYSDRIQVLQNMVHCADLSNP
  TKPLQLYRQWTDRIMEEFFRQGDRERERGMEISPMCDKHNASVEKSQVGFIDYIVHPLWETWADLVHPDA
  QDILDTLEDNREWYQSTIPQSPSPAPDDPEEGRQGQTEKFQFELTLEEDGESDTEKDSGSQVEEDTSCSD
  SKTLCTQDSESTEIPLDEQVEEEAVGEEEESQPEACVIDDRSPDT
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001197218
• ORF Size: 2235 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001197218.2
• RefSeq Size: 7979 bp
• RefSeq ORF: 2238 bp
• Locus ID: 5144
• UniProt ID: Q08499
• Cytogenetics: 5q11.2-q12.1
• Protein Families: Druggable Genome
• Protein Pathways: Progesterone-mediated oocyte maturation, Purine metabolism
• Gene Summary: This gene encodes one of four mammalian counterparts to the fruit fly 'dunce' gene. The encoded protein has 3',5'-cyclic-AMP phosphodiesterase activity and degrades cAMP, which acts as a signal transduction molecule in multiple cell types. This gene uses different promoters to generate multiple alternatively spliced transcript variants that encode functional proteins.[provided by RefSeq, Sep 2009]