DHX35 (NM_001190809) Human Tagged ORF Clone

CAT#: RG231366

DHX35 (tGFP-tagged) - Human DEAH (Asp-Glu-Ala-His) box polypeptide 35 (DHX35), transcript variant 2

Product Images

Circular map for RG231366

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DHX35
• Synonyms: C20orf15; DDX35; KAIA0875
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG231366 representing NM_001190809
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCGCCCGTGGGACCGGTGAAGTTCTGGCGACCCGGTACAGAGGGGCCAGGTGTAAGCATCTCTG
  AAGAGAGACAAAGTCTGGCTGAAAACTCTGGGACAACGGTTGTTTACAACCCTTATGCTGCCCTTTCCAT
  AGAGCAGCAGAGGCAGAAGCTGCCGGTATTCAAGTACCTTGCAGAAGCCGGCTGGACAGCTGAAGGAAGA
  GTGGTAGGAGTGACCCAGCCTCGAAGAGTGGCTGCTGTTACAGTTGCAGGGAGAGTAGCTGAAGAAAGGG
  GTGCAGTGCTGGGCCACGAGGTGGGCTACTGCATCCGCTTTGATGACTGCACCGACCAGCTGGCCACGAG
  AATTAAGTTTCTTACTGATGGAATGCTGGTCAGGGAAATGATGGTTGATCCGTTGTTAACAAAATATAGT
  GTCATCATGCTGGATGAAGCCCACGAGAGGACCTTGTACACTGACATTGCCATTGGCTTGCTAAAAAAGA
  TTCAGAAAAAGCGAGGGGATCTTCGATTGATTGTAGCTTCAGCCACTCTGGATGCAGACAAATTCCGGGA
  TTTCTTTAATCAAAATGAAACCAGTGATCCAGCAAGGGATACATGTGTGATCCTTACAGTGGAAGGGAGA
  ACATTTCCGGTGGATATCTTTTATCTACAAAGTCCTGTTCCAGATTATATCAAATCAACTGTCGAAACTG
  TGGTGAAAATTCACCAGACAGAGGGAGACGGAGACGTTTTAGCATTTCTTACTGGCCAGGAAGAGGTAGA
  AACTGTTGTGTCGATGCTCATCGAGCAGGCTCGAGCACTAGCTCGCACTGGGATGAAGAGACACCTCCGA
  GTTCTCCCCATGTATGCAGGACTGCCTTCCTTTGAGCAAATGAAAGTGTTTGAAAGGGTGTCACGCAGTG
  TCAGAAAGGTGATAGTGGCCACCAATGTGGCAGAAACCTCTATCACAATCAGCGGCATTGTGTATGTGAT
  CGACTGTGGCTTTGTGAAACTCCGAGCCTACAATCCCAGGACAGCTATTGAATGCTTGGTGGTGGTGCCA
  GTCTCCCAGGCATCAGCTAATCAGCGAGCAGGACGTGGTGGTCGTAGTCGCTCGGGAAAATGTTATCGCC
  TTTATACAGAGGAAGCCTTTGACAAGTTGCCTCAGTCTACGGTTCCTGAGATGCAGCGTAGTAATTTGGC
  ACCTGTCATCCTGCAGCTGAAAGCACTAGGAATTGACAATGTCCTCAGGTTCCACTTCATGTCGCCCCCT
  CCAGCACAGTCGATGGTTCAAGCCTTGGAGTTACTGTATGCTCTGGGAGGTCTGGACAAAGACTGTCGCC
  TAACTGAACCGCTTGGCATGAGAATTGCAGAGTTTCCTTTGAATCCCATGTTTGCCAAAATGCTGCTTGA
  ATCAGGAAACTTCGGCTGTTCTCAGGAAATTCTAAGCATCGCTGCCATGATGCAGATCCAGAATATCTTT
  GTGGTCCCCCCAAACCAGAAGTCTCACGCAATTCGAGTGCACCGTAAATTTGCTGTGGAGGAGGGCGACC
  ACCTCACTATGCTCAATATATATGAAGCATTTATCAAACACAATAAGGACTCTAAATGGTGTCAGGAACA
  TTTCCTGAATTACAAGGGTCTTGTCAGAGCTGCGACTGTAAGAGAACAATTGAAAAAGCTTCTTGTCAAG
  TTTCAAGTGCCCAGGAAGTCTAGTGAAGGTGACCCGGATCTGGTTCTGAGGTGCATTGTCTCCGGCTTCT
  TCGCCAATGCAGCGAGGTTTCATTCTACTGGAGCTTATAGGACCATCCGTGATGACCATGAGCTGCACAT
  ACACCCTGCGTCAGTCCTCTATGCAGAGAAGCCGCCTCGCTGGGTCATCTATAACGAAGTTATACAGACC
  TCCAAGTACTACATGAGAGATGTGACTGCCATTGAATCGGCCTGGCTGTTGGAGCTGGCTCCACACTTTT
  ATCAACAAGGAACGCACCTGTCTCTGAAAGCCAAAAGGGCCAAGGTCCAGGACCCG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG231366 representing NM_001190809
  Red=Cloning site Green=Tags(s)
  
  MAAPVGPVKFWRPGTEGPGVSISEERQSLAENSGTTVVYNPYAALSIEQQRQKLPVFKYLAEAGWTAEGR
  VVGVTQPRRVAAVTVAGRVAEERGAVLGHEVGYCIRFDDCTDQLATRIKFLTDGMLVREMMVDPLLTKYS
  VIMLDEAHERTLYTDIAIGLLKKIQKKRGDLRLIVASATLDADKFRDFFNQNETSDPARDTCVILTVEGR
  TFPVDIFYLQSPVPDYIKSTVETVVKIHQTEGDGDVLAFLTGQEEVETVVSMLIEQARALARTGMKRHLR
  VLPMYAGLPSFEQMKVFERVSRSVRKVIVATNVAETSITISGIVYVIDCGFVKLRAYNPRTAIECLVVVP
  VSQASANQRAGRGGRSRSGKCYRLYTEEAFDKLPQSTVPEMQRSNLAPVILQLKALGIDNVLRFHFMSPP
  PAQSMVQALELLYALGGLDKDCRLTEPLGMRIAEFPLNPMFAKMLLESGNFGCSQEILSIAAMMQIQNIF
  VVPPNQKSHAIRVHRKFAVEEGDHLTMLNIYEAFIKHNKDSKWCQEHFLNYKGLVRAATVREQLKKLLVK
  FQVPRKSSEGDPDLVLRCIVSGFFANAARFHSTGAYRTIRDDHELHIHPASVLYAEKPPRWVIYNEVIQT
  SKYYMRDVTAIESAWLLELAPHFYQQGTHLSLKAKRAKVQDP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001190809
• ORF Size: 2016 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001190809.2
• RefSeq Size: 3243 bp
• RefSeq ORF: 2019 bp
• Locus ID: 60625
• UniProt ID: Q9H5Z1
• Cytogenetics: 20q11.23-q12
• Gene Summary: DEAD box proteins characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The function of this gene product which is a member of this family, has not been determined. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jun 2010]