WI2 (NM_001195127) Human Tagged ORF Clone

CAT#: RG231080

LOC100288524 (tGFP-tagged) - Human forkhead box L1-like (LOC100288524)

Product Images

Circular map for RG231080

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: WI2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG231080 representing NM_001195127
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTGACAGCTCGCAGTATCCCTACAACTGCTTCAATTACGACGCCGACGACTACCCCGCCGGCAGCT
  CCGACGAAGACAAGAGGCTCACGCGGCCCGCGTACAGCTACATCGCCTTGATCGCCATGGCCATTCAGCA
  GAGCCCCGCGGGGAGGGTGACCCTGTCCGGCATCTACGACTTCATCATGCGCAAATTCCCCTATTACCGC
  GCCAACCAGCGCGCCTGGCAGAACTCCATCCGCCACAACCTGTCCCTCAACAGCTGCTTCGTCAAGGTGC
  CGCGGTCCGAGGGCCACGAGAAGGGCAAAGGCAACTACTGGACGTTCGCGGGCGGCTGCGAGTCGCTGCT
  GGACCTCTTCGAGAACGGCAACTACCGGCGGCGGCGGCGGCGGCGCGGCCCCAAGCGCGAGGGGCCGAGG
  GGTCCGCGCGCGGGGGGCGCCCAGGGGCCGTCGGGTCCGTCCGAGCCGCCCGCCGCTCAGGGGCGCCTGG
  CCCCGGACAGCGCTGGCGAGGGCGCCCCGGGCCGTGAGCCCCCCGCCAGCCCCGCTCCCCCGGGGAAGGA
  GCACCCCCGGGACCTCAAGTTCAGCATCGACTACATCCTGTCCTCCCCAGACCCCTTCCCTGGGCTCAAG
  CCGCCCTGCCTCGCACAAGAGGGCAGATACCCGCGGCTGGAGAACGTGGGACTCCACTTTTGGACAATG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG231080 representing NM_001195127
  Red=Cloning site Green=Tags(s)
  
  MFDSSQYPYNCFNYDADDYPAGSSDEDKRLTRPAYSYIALIAMAIQQSPAGRVTLSGIYDFIMRKFPYYR
  ANQRAWQNSIRHNLSLNSCFVKVPRSEGHEKGKGNYWTFAGGCESLLDLFENGNYRRRRRRRGPKREGPR
  GPRAGGAQGPSGPSEPPAAQGRLAPDSAGEGAPGREPPASPAPPGKEHPRDLKFSIDYILSSPDPFPGLK
  PPCLAQEGRYPRLENVGLHFWTM
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001195127
• ORF Size: 699 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001195127.1, NP_001182056.1
• RefSeq Size: 702 bp
• RefSeq ORF: 702 bp
• Locus ID: 100288524
• Cytogenetics: 7p22.3