PARD3 (NM_001184785) Human Tagged ORF Clone

CAT#: RG230706

PARD3 (tGFP-tagged) - Human par-3 partitioning defective 3 homolog (C. elegans) (PARD3), transcript variant 2

Product Images

Circular map for RG230706

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PARD3
• Synonyms: ASIP; Baz; PAR3; PAR3alpha; PARD-3; PARD3A; PPP1R118; SE2-5L16; SE2-5LT1; SE2-5T2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG230706 representing NM_001184785
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAGTGACCGTGTGCTTCGGACGGACCCGGGTGGTCGTGCCGTGCGGGGACGGCCACATGAAAGTTT
  TCAGCCTCATCCAGCAGGCGGTGACCCGCTACCGGAAGGCCATCGCCAAGGATCCAAACTACTGGATACA
  GGTGCATCGCTTGGAACATGGAGATGGAGGAATACTAGACCTTGATGACATTCTTTGTGATGTAGCAGAC
  GATAAAGACAGACTGGTAGCAGTGTTTGATGAGCAGGATCCACATCACGGAGGTGATGGCACCAGTGCCA
  GTTCCACGGGTACCCAGAGCCCAGAGATATTTGGTAGTGAGCTTGGCACCAACAATGTCTCAGCCTTTCA
  GCCTTACCAAGCAACAAGTGAAATTGAGGTCACACCTTCAGTCCTTCGAGCAAATATGCCTCTTCATGTT
  CGACGCAGTAGTGACCCAGCTCTAATTGGCCTCTCCACTTCTGTCAGTGATAGTAATTTTTCCTCTGAAG
  AGCCTTCAAGGAAAAATCCCACACGCTGGTCAACAACAGCTGGCTTCCTCAAGCAGAACACTGCTGGGAG
  TCCTAAAACCTGCGACAGGAAGAAAGATGAAAACTACAGAAGCCTCCCGCGGGATACTAGTAACTGGTCT
  AACCAATTTCAGAGAGACAATGCTCGCTCGTCTCTGAGTGCCAGTCACCCAATGGTGGGCAAGTGGCTGG
  AGAAACAAGAACAGGATGAGGATGGGACAGAAGAGGATAACAGTCGTGTTGAACCTGTTGGACATGCTGA
  CACGGGTTTGGAGCATATACCCAACTTTTCTCTGGATGATATGGTAAAGCTCGTAGAAGTCCCCAACGAT
  GGAGGGCCTCTGGGAATCCATGTAGTGCCTTTCAGTGCTCGAGGCGGCAGAACCCTGGGGTTATTAGTAA
  AACGATTGGAGAAAGGTGGTAAAGCTGAACATGAAAATCTTTTTCGTGAGAATGATTGCATTGTCAGGAT
  TAATGATGGCGACCTTCGAAATAGAAGATTTGAACAAGCACAACATATGTTTCGCCAAGCCATGCGTACA
  CCCATCATTTGGTTCCATGTGGTTCCTGCAGCAAATAAAGAGCAGTATGAACAACTATCCCAAAGTGAGA
  AGAACAATTACTATTCAAGCCGTTTTAGCCCTGACAGCCAGTATATTGACAACAGGAGTGTGAACAGTGC
  AGGGCTTCACACGGTGCAGAGAGCACCCCGACTGAACCACCCGCCTGAGCAGATAGACTCTCACTCAAGA
  CTACCTCATAGCGCACACCCCTCGGGAAAACCACCATCCGCTCCAGCCTCGGCACCTCAGAATGTATTTA
  GTACGACTGTAAGCAGTGGTTATAACACCAAAAAAATAGGCAAGAGGCTTAATATCCAGCTTAAGAAAGG
  TACAGAAGGTTTGGGATTCAGCATCACTTCCAGAGATGTAACAATAGGTGGCTCAGCTCCAATCTATGTG
  AAAAACATTCTCCCCCGGGGGGCGGCCATTCAGGATGGCCGACTTAAGGCAGGAGACAGACTTATAGAGG
  TAAATGGAGTAGATTTAGTGGGCAAATCCCAAGAGGAAGTTGTTTCGCTGTTGAGAAGCACCAAGATGGA
  AGGAACTGTGAGCCTTCTGGTCTTTCGCCAGGAAGACGCCTTCCACCCAAGGGAACTGAATGCAGAGCCA
  AGCCAGATGCAGATTCCAAAAGAAACGAAAGCAGAAGATGAGGATATTGTTCTTACACCTGATGGCACCA
  GGGAATTTCTGACATTTGAAGTCCCACTTAATGATTCAGGATCTGCAGGCCTTGGTGTCAGTGTCAAAGG
  TAACCGGTCAAAAGAGAACCACGCAGATTTGGGAATCTTTGTCAAGTCCATTATTAATGGAGGAGCAGCA
  TCTAAAGATGGAAGGCTTCGGGTGAATGATCAACTGATAGCAGTAAATGGAGAATCCCTGTTGGGCAAGA
  CAAACCAAGATGCCATGGAAACCCTAAGAAGGTCTATGTCTACTGAAGGCAATAAACGAGGAATGATCCA
  GCTTATTGTTGCAAGGAGAATAAGCAAGTGCAATGAGCTGAAGTCACCTGGGAGCCCCCCTGGACCTGAG
  CTGCCCATTGAAACAGCGTTGGATGATAGAGAACGAAGAATTTCCCATTCCCTCTACAGTGGGATTGAGG
  GGCTTGATGAATCGCCCAGCAGAAATGCTGCCCTCAGTAGGATAATGGGTAAATACCAGCTGTCCCCTAC
  AGTGAATATGCCCCAAGATGACACTGTCATTATAGAAGATGACAGGTTGCCAGTGCTTCCTCCACATCTC
  TCTGACCAGTCCTCTTCCAGCTCCCATGATGATGTGGGGTTTGTGACGGCAGATGCTGGTACTTGGGCCA
  AGGCTGCAATCAGTGATTCAGCCGACTGCTCTTTGAGTCCAGATGTTGATCCAGTTCTTGCTTTTCAACG
  AGAAGGATTTGGACGTCAGAGTATGTCAGAAAAACGCACAAAGCAATTTTCAGATGCCAGTCAATTGGAT
  TTCGTTAAAACACGAAAATCAAAAAGCATGGATTTAGGTATAGCTGACGAGACTAAACTCAATACAGTGG
  ATGACCAGAAAGCAGGTTCTCCCAGCAGAGATGTGGGTCCTTCCCTGGGTCTGAAGAAGTCAAGCTCGTT
  GGAGAGTCTGCAGACCGCAGTTGCCGAGGTGACTTTGAATGGGGATATTCCTTTCCATCGTCCACGGCCG
  CGGATAATCAGAGGCAGGGGATGCAATGAGAGCTTCAGAGCTGCCATCGACAAATCTTATGATAAACCCG
  CGGTAGATGATGATGATGAAGGCATGGAGACCTTGGAAGAAGACACAGAAGAAAGTTCAAGATCAGGGAG
  AGAGTCTGTATCCACAGCCAGTGATCAGCCTTCCCACTCTCTGGAGAGACAAATGAATGGAAACCAAGAG
  AAAGGTGATAAGACTGATAGAAAAAAGGATAAAACTGGAAAAGAAAAGAAGAAAGATAGAGATAAGGAGA
  AGGATAAAATGAAAGCCAAGAAGGGAATGCTGAAGGGCTTGGGAGACATGTTCAGGTTTGGCAAACATCG
  AAAAGATGACAAGATTGAGAAAACGGGTAAAATAAAAATACAGGAATCCTTTACATCAGAAGAGGAGAGG
  ATACGAATGAAGCAGGAGCAGGAGAGGATTCAAGCCAAAACTCGAGAATTTAGGGAACGACAAGCTCGAG
  AGCGTGACTATGCTGAAATTCAAGATTTTCATCGGACATTTGGCTGTGATGATGAGTTAATGTATGGGGG
  AGTTTCTTCTTATGAAGGTTCCATGGCTCTCAACGCTAGACCTCAGAGCCCACGAGAAGGGCATATGATG
  GATGCTTTGTATGCCCAAGTCAAGAAGCCGCGGAATTCCAAACCCTCACCTGTAGACAGTAACAGATCAA
  CTCCTAGCAATCATGATCGGATACAGCGTCTGAGGCAAGAATTTCAGCAAGCAAAGCAAGATGAAGATGT
  AGAAGATCGTCGGCGGACCTATAGTTTTGAGCAACCCTGGCCGAACGCACGGCCGGCGACGCAGAGCGGG
  CGACACTCGGTGTCCGTGGAGGTGCAGATGCAGCGGCAGCGGCAGGAGGAGCGCGAGAGCTCCCAGCAGG
  CCCAGCGCCAGTACAGCTCTCTGCCTCGGCAAAGCAGGAAAAATGCCAGCTCGGTCTCCCAGGACTCTTG
  GGAGCAGAACTACTCCCCTGGGGAAGGCTTCCAGAGTGCCAAAGAGAACCCCAGGTACTCCAGCTACCAA
  GGCTCCAGGAACGGCTACCTGGGAGGACATGGCTTCAACGCCAGGGTCATGCTGGAAACTCAGGAGCTCC
  TTCGCCAGGAACAGAGGCGGAAGGAGCAGCAGATGAAGAAGCAGCCTCCTTCCGAGGGGCCCAGCAACTA
  TGACTCGTATAAGAAAGTCCAGGACCCCAGTTACGCCCCTCCCAAGGGGCCCTTCCGGCAAGATGTGCCC
  CCCTCCCCTTCTCAGGTTGCGAGGCTGAACAGACTTCAGACTCCTGAGAAAGGGAGGCCCTTCTATTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG230706 representing NM_001184785
  Red=Cloning site Green=Tags(s)
  
  MKVTVCFGRTRVVVPCGDGHMKVFSLIQQAVTRYRKAIAKDPNYWIQVHRLEHGDGGILDLDDILCDVAD
  DKDRLVAVFDEQDPHHGGDGTSASSTGTQSPEIFGSELGTNNVSAFQPYQATSEIEVTPSVLRANMPLHV
  RRSSDPALIGLSTSVSDSNFSSEEPSRKNPTRWSTTAGFLKQNTAGSPKTCDRKKDENYRSLPRDTSNWS
  NQFQRDNARSSLSASHPMVGKWLEKQEQDEDGTEEDNSRVEPVGHADTGLEHIPNFSLDDMVKLVEVPND
  GGPLGIHVVPFSARGGRTLGLLVKRLEKGGKAEHENLFRENDCIVRINDGDLRNRRFEQAQHMFRQAMRT
  PIIWFHVVPAANKEQYEQLSQSEKNNYYSSRFSPDSQYIDNRSVNSAGLHTVQRAPRLNHPPEQIDSHSR
  LPHSAHPSGKPPSAPASAPQNVFSTTVSSGYNTKKIGKRLNIQLKKGTEGLGFSITSRDVTIGGSAPIYV
  KNILPRGAAIQDGRLKAGDRLIEVNGVDLVGKSQEEVVSLLRSTKMEGTVSLLVFRQEDAFHPRELNAEP
  SQMQIPKETKAEDEDIVLTPDGTREFLTFEVPLNDSGSAGLGVSVKGNRSKENHADLGIFVKSIINGGAA
  SKDGRLRVNDQLIAVNGESLLGKTNQDAMETLRRSMSTEGNKRGMIQLIVARRISKCNELKSPGSPPGPE
  LPIETALDDRERRISHSLYSGIEGLDESPSRNAALSRIMGKYQLSPTVNMPQDDTVIIEDDRLPVLPPHL
  SDQSSSSSHDDVGFVTADAGTWAKAAISDSADCSLSPDVDPVLAFQREGFGRQSMSEKRTKQFSDASQLD
  FVKTRKSKSMDLGIADETKLNTVDDQKAGSPSRDVGPSLGLKKSSSLESLQTAVAEVTLNGDIPFHRPRP
  RIIRGRGCNESFRAAIDKSYDKPAVDDDDEGMETLEEDTEESSRSGRESVSTASDQPSHSLERQMNGNQE
  KGDKTDRKKDKTGKEKKKDRDKEKDKMKAKKGMLKGLGDMFRFGKHRKDDKIEKTGKIKIQESFTSEEER
  IRMKQEQERIQAKTREFRERQARERDYAEIQDFHRTFGCDDELMYGGVSSYEGSMALNARPQSPREGHMM
  DALYAQVKKPRNSKPSPVDSNRSTPSNHDRIQRLRQEFQQAKQDEDVEDRRRTYSFEQPWPNARPATQSG
  RHSVSVEVQMQRQRQEERESSQQAQRQYSSLPRQSRKNASSVSQDSWEQNYSPGEGFQSAKENPRYSSYQ
  GSRNGYLGGHGFNARVMLETQELLRQEQRRKEQQMKKQPPSEGPSNYDSYKKVQDPSYAPPKGPFRQDVP
  PSPSQVARLNRLQTPEKGRPFYS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001184785
• ORF Size: 4059 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001184785.1, NP_001171714.1
• RefSeq Size: 6004 bp
• RefSeq ORF: 4062 bp
• Locus ID: 56288
• UniProt ID: Q8TEW0
• Cytogenetics: 10p11.22-p11.21
• Protein Pathways: Adherens junction, Chemokine signaling pathway, Endocytosis, Neuroactive ligand-receptor interaction, Tight junction
• Gene Summary: This gene encodes a member of the PARD protein family. PARD family members interact with other PARD family members and other proteins; they affect asymmetrical cell division and direct polarized cell growth. Multiple alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Oct 2011]