PARD3 (NM_001184791) Human Tagged ORF Clone

CAT#: RG230680

PARD3 (tGFP-tagged) - Human par-3 partitioning defective 3 homolog (C. elegans) (PARD3), transcript variant 8

Product Images

Circular map for RG230680

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PARD3
• Synonyms: ASIP; Baz; PAR3; PAR3alpha; PARD-3; PARD3A; PPP1R118; SE2-5L16; SE2-5LT1; SE2-5T2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG230680 representing NM_001184791
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAGTGACCGTGTGCTTCGGACGGACCCGGGTGGTCGTGCCGTGCGGGGACGGCCACATGAAAGTTT
  TCAGCCTCATCCAGCAGGCGGTGACCCGCTACCGGAAGGCCATCGCCAAGGATCCAAACTACTGGATACA
  GGTGCATCGCTTGGAACATGGAGATGGAGGAATACTAGACCTTGATGACATTCTTTGTGATGTAGCAGAC
  GATAAAGACAGACTGGTAGCAGTGTTTGATGAGCAGGATCCACATCACGGAGGTGATGGCACCAGTGCCA
  GTTCCACGGGTACCCAGAGCCCAGAGATATTTGGTAGTGAGCTTGGCACCAACAATGTCTCAGCCTTTCA
  GCCTTACCAAGCAACAAGTGAAATTGAGGTCACACCTTCAGTCCTTCGAGCAAATATGCCTCTTCATGTT
  CGACGCAGTAGTGACCCAGCTCTAATTGGCCTCTCCACTTCTGTCAGTGATAGTAATTTTTCCTCTGAAG
  AGCCTTCAAGGAAAAATCCCACACGCTGGTCAACAACAGCTGGCTTCCTCAAGCAGAACACTGCTGGGAG
  TCCTAAAACCTGCGACAGGAAGGATGAGGATGGGACAGAAGAGGATAACAGTCGTGTTGAACCTGTTGGA
  CATGCTGACACGGGTTTGGAGCATATACCCAACTTTTCTCTGGATGATATGGTAAAGCTCGTAGAAGTCC
  CCAACGATGGAGGGCCTCTGGGAATCCATGTAGTGCCTTTCAGTGCTCGAGGCGGCAGAACCCTGGGGTT
  ATTAGTAAAACGATTGGAGAAAGGTGGTAAAGCTGAACATGAAAATCTTTTTCGTGAGAATGATTGCATT
  GTCAGGATTAATGATGGCGACCTTCGAAATAGAAGATTTGAACAAGCACAACATATGTTTCGCCAAGCCA
  TGCGTACACCCATCATTTGGTTCCATGTGGTTCCTGCAGCAAATAAAGAGCAGTATGAACAACTATCCCA
  AAGTGAGAAGAACAATTACTATTCAAGCCGTTTTAGCCCTGACAGCCAGTATATTGACAACAGGAGTGTG
  AACAGTGCAGGGCTTCACACGGTGCAGAGAGCACCCCGACTGAACCACCCGCCTGAGCAGATAGACTCTC
  ACTCAAGACTACCTCATAGCGCACACCCCTCGGGAAAACCACCATCCGCTCCAGCCTCGGCACCTCAGAA
  TGTATTTAGTACGACTGTAAGCAGTGGTTATAACACCAAAAAAATAGGCAAGAGGCTTAATATCCAGCTT
  AAGAAAGGTACAGAAGGTTTGGGATTCAGCATCACTTCCAGAGATGTAACAATAGGTGGCTCAGCTCCAA
  TCTATGTGAAAAACATTCTCCCCCGGGGGGCGGCCATTCAGGATGGCCGACTTAAGGCAGGAGACAGACT
  TATAGAGGTAAATGGAGTAGATTTAGTGGGCAAATCCCAAGAGGAAGTTGTTTCGCTGTTGAGAAGCACC
  AAGATGGAAGGAACTGTGAGCCTTCTGGTCTTTCGCCAGGAAGACGCCTTCCACCCAAGGGAACTGAAAG
  CAGAAGATGAGGATATTGTTCTTACACCTGATGGCACCAGGGAATTTCTGACATTTGAAGTCCCACTTAA
  TGATTCAGGATCTGCAGGCCTTGGTGTCAGTGTCAAAGGTAACCGGTCAAAAGAGAACCACGCAGATTTG
  GGAATCTTTGTCAAGTCCATTATTAATGGAGGAGCAGCATCTAAAGATGGAAGGCTTCGGGTGAATGATC
  AACTGATAGCAGTAAATGGAGAATCCCTGTTGGGCAAGACAAACCAAGATGCCATGGAAACCCTAAGAAG
  GTCTATGTCTACTGAAGGCAATAAACGAGGAATGATCCAGCTTATTGTTGCAAGGAGAATAAGCAAGTGC
  AATGAGCTGAAGTCACCTGGGAGCCCCCCTGGACCTGAGCTGCCCATTGAAACAGCGTTGGATGATAGAG
  AACGAAGAATTTCCCATTCCCTCTACAGTGGGATTGAGGGGCTTGATGAATCGCCCAGCAGAAATGCTGC
  CCTCAGTAGGATAATGGGTAAATACCAGCTGTCCCCTACAGTGAATATGCCCCAAGATGACACTGTCATT
  ATAGAAGATGACAGGTTGCCAGTGCTTCCTCCACATCTCTCTGACCAGTCCTCTTCCAGCTCCCATGATG
  ATGTGGGGTTTGTGACGGCAGATGCTGGTACTTGGGCCAAGGCTGCAATCAGTGATTCAGCCGACTGCTC
  TTTGAGTCCAGATGTTGATCCAGTTCTTGCTTTTCAACGAGAAGGATTTGGACGTCAGAGTATGTCAGAA
  AAACGCACAAAGCAATTTTCAGATGCCAGTCAATTGGATTTCGTTAAAACACGAAAATCAAAAAGCATGG
  ATTTAGGTAGTTCTCCCAGCAGAGATGTGGGTCCTTCCCTGGGTCTGAAGAAGTCAAGCTCGTTGGAGAG
  TCTGCAGACCGCAGTTGCCGAGGTGACTTTGAATGGGGATATTCCTTTCCATCGTCCACGGCCGCGGATA
  ATCAGAGGCAGGGGATGCAATGAGAGCTTCAGAGCTGCCATCGACAAATCTTATGATAAACCCGCGGTAG
  ATGATGATGATGAAGGCATGGAGACCTTGGAAGAAGACACAGAAGAAAGTTCAAGATCAGGGAGAGAGTC
  TGTATCCACAGCCAGTGATCAGCCTTCCCACTCTCTGGAGAGACAAATGAATGGAAACCAAGAGAAAGGT
  GATAAGACTGATAGAAAAAAGGATAAAACTGGAAAAGAAAAGAAGAAAGATAGAGATAAGGAGAAGGATA
  AAATGAAAGCCAAGAAGGGAATGCTGAAGGGCTTGGGAGACATGTTCAGGATTCAAGCCAAAACTCGAGA
  ATTTAGGGAACGACAAGCTCGAGAGCGTGACTATGCTGAAATTCAAGATTTTCATCGGACATTTGGCTGT
  GATGATGAGTTAATGTATGGGGGAGTTTCTTCTTATGAAGGTTCCATGGCTCTCAACGCTAGACCTCAGA
  GCCCACGAGAAGGGCATATGATGGATGCTTTGTATGCCCAAGTCAAGAAGCCGCGGAATTCCAAACCCTC
  ACCTGTAGACAGTAACAGATCAACTCCTAGCAATCATGATCGGATACAGCGTCTGAGGCAAGAATTTCAG
  CAAGCAAAGCAAGATGAAGATGTAGAAGATCGTCGGCGGACCTATAGTTTTGAGCAACCCTGGCCGAACG
  CACGGCCGGCGACGCAGAGCGGGCGACACTCGGTGTCCGTGGAGGTGCAGATGCAGCGGCAGCGGCAGGA
  GGAGCGCGAGAGCTCCCAGCAGGCCCAGCGCCAGTACAGCTCTCTGCCTCGGCAAAGCAGGAAAAATGCC
  AGCTCGGTCTCCCAGGACTCTTGGGAGCAGAACTACTCCCCTGGGGAAGGCTTCCAGAGTGCCAAAGAGA
  ACCCCAGGTACTCCAGCTACCAAGGCTCCAGGAACGGCTACCTGGGAGGACATGGCTTCAACGCCAGGGT
  CATGCTGGAAACTCAGGAGCTCCTTCGCCAGGAACAGAGGCGGAAGGAGCAGCAGATGAAGAAGCAGCCT
  CCTTCCGAGGGGCCCAGCAACTATGACTCGTATAAGAAAGTCCAGGACCCCAGTTACGCCCCTCCCAAGG
  GGCCCTTCCGGCAAGATGTGCCCCCCTCCCCTTCTCAGGTTGCGAGGCTGAACAGACTTCAGACTCCTGA
  GAAAGGGAGGCCCTTCTATTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG230680 representing NM_001184791
  Red=Cloning site Green=Tags(s)
  
  MKVTVCFGRTRVVVPCGDGHMKVFSLIQQAVTRYRKAIAKDPNYWIQVHRLEHGDGGILDLDDILCDVAD
  DKDRLVAVFDEQDPHHGGDGTSASSTGTQSPEIFGSELGTNNVSAFQPYQATSEIEVTPSVLRANMPLHV
  RRSSDPALIGLSTSVSDSNFSSEEPSRKNPTRWSTTAGFLKQNTAGSPKTCDRKDEDGTEEDNSRVEPVG
  HADTGLEHIPNFSLDDMVKLVEVPNDGGPLGIHVVPFSARGGRTLGLLVKRLEKGGKAEHENLFRENDCI
  VRINDGDLRNRRFEQAQHMFRQAMRTPIIWFHVVPAANKEQYEQLSQSEKNNYYSSRFSPDSQYIDNRSV
  NSAGLHTVQRAPRLNHPPEQIDSHSRLPHSAHPSGKPPSAPASAPQNVFSTTVSSGYNTKKIGKRLNIQL
  KKGTEGLGFSITSRDVTIGGSAPIYVKNILPRGAAIQDGRLKAGDRLIEVNGVDLVGKSQEEVVSLLRST
  KMEGTVSLLVFRQEDAFHPRELKAEDEDIVLTPDGTREFLTFEVPLNDSGSAGLGVSVKGNRSKENHADL
  GIFVKSIINGGAASKDGRLRVNDQLIAVNGESLLGKTNQDAMETLRRSMSTEGNKRGMIQLIVARRISKC
  NELKSPGSPPGPELPIETALDDRERRISHSLYSGIEGLDESPSRNAALSRIMGKYQLSPTVNMPQDDTVI
  IEDDRLPVLPPHLSDQSSSSSHDDVGFVTADAGTWAKAAISDSADCSLSPDVDPVLAFQREGFGRQSMSE
  KRTKQFSDASQLDFVKTRKSKSMDLGSSPSRDVGPSLGLKKSSSLESLQTAVAEVTLNGDIPFHRPRPRI
  IRGRGCNESFRAAIDKSYDKPAVDDDDEGMETLEEDTEESSRSGRESVSTASDQPSHSLERQMNGNQEKG
  DKTDRKKDKTGKEKKKDRDKEKDKMKAKKGMLKGLGDMFRIQAKTREFRERQARERDYAEIQDFHRTFGC
  DDELMYGGVSSYEGSMALNARPQSPREGHMMDALYAQVKKPRNSKPSPVDSNRSTPSNHDRIQRLRQEFQ
  QAKQDEDVEDRRRTYSFEQPWPNARPATQSGRHSVSVEVQMQRQRQEERESSQQAQRQYSSLPRQSRKNA
  SSVSQDSWEQNYSPGEGFQSAKENPRYSSYQGSRNGYLGGHGFNARVMLETQELLRQEQRRKEQQMKKQP
  PSEGPSNYDSYKKVQDPSYAPPKGPFRQDVPPSPSQVARLNRLQTPEKGRPFYS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001184791
• ORF Size: 3732 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001184791.1, NP_001171720.1
• RefSeq Size: 5677 bp
• RefSeq ORF: 3735 bp
• Locus ID: 56288
• UniProt ID: Q8TEW0
• Cytogenetics: 10p11.22-p11.21
• Protein Pathways: Adherens junction, Chemokine signaling pathway, Endocytosis, Neuroactive ligand-receptor interaction, Tight junction
• Gene Summary: This gene encodes a member of the PARD protein family. PARD family members interact with other PARD family members and other proteins; they affect asymmetrical cell division and direct polarized cell growth. Multiple alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Oct 2011]