AREB6 (ZEB1) (NM_001174094) Human Tagged ORF Clone

CAT#: RG230651

ZEB1 (tGFP-tagged) - Human zinc finger E-box binding homeobox 1 (ZEB1), transcript variant 7

Product Images

Circular map for RG230651

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: AREB6
• Synonyms: AREB6; BZP; DELTAEF1; FECD6; NIL2A; PPCD3; TCF8; ZFHEP; ZFHX1A
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG230651 representing NM_001174094
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAGTTACAAATTATAATACTGTGGTAGAAACAAATTCAGATTCAGATGATGAAGACAAACTGCATA
  TTGTGGAAGAAGAAAGTGTTACAGATGCAGCTGACTGTGAAGGTGTACCAGAGGATGACCTGCCAACAGA
  CCAGACAGTGTTACCAGGGAGGAGCAGTGAAAGAGAAGGGAATGCTAAGAACTGCTGGGAGGATGACAGA
  AAGGAAGGGCAAGAAATCCTGGGGCCTGAAGCTCAGGCAGATGAAGCAGGATGTACAGTAAAAGATGATG
  AATGCGAGTCAGATGCAGAAAATGAGCAAAACCATGATCCTAATGTTGAAGAGTTTCTACAACAACAAGA
  CACTGCTGTCATTTTTCCTGAGGCACCTGAAGAGGACCAGAGGCAGGGCACACCAGAAGCCAGTGGTCAT
  GATGAAAATGGAACACCAGATGCATTTTCACAATTACTCACCTGTCCATATTGTGATAGAGGCTATAAAC
  GCTTTACCTCTCTGAAAGAACACATTAAATATCGTCATGAAAAGAATGAAGATAACTTTAGTTGCTCCCT
  GTGCAGTTACACCTTTGCATACAGAACCCAACTTGAACGTCACATGACATCACATAAATCAGGAAGAGAT
  CAAAGACATGTGACGCAGTCTGGGTGTAATCGTAAATTCAAATGCACTGAGTGTGGAAAAGCTTTCAAAT
  ACAAACATCACCTAAAAGAGCACTTAAGAATTCACAGTGGAGAGAAGCCATATGAATGCCCAAACTGCAA
  GAAACGCTTTTCCCATTCTGGCTCCTATAGCTCACACATAAGCAGTAAGAAATGTATCAGCTTGATACCT
  GTGAATGGGCGACCAAGAACAGGACTCAAGACATCTCAGTGTTCTTCACCGTCTCTTTCAGCATCACCAG
  GCAGTCCCACACGACCACAGATACGGCAAAAGATAGAGAATAAACCCCTTCAAGAACAACTTTCTGTTAA
  CCAAATTAAAACTGAACCTGTGGATTATGAATTCAAACCCATAGTGGTTGCTTCAGGAATCAACTGTTCA
  ACCCCTTTACAAAATGGGGTTTTCACTGGTGGTGGCCCATTACAGGCAACCAGTTCTCCTCAGGGCATGG
  TGCAAGCTGTTGTTCTGCCAACAGTTGGTTTGGTGTCTCCCATAAGTATCAATTTAAGTGATATTCAGAA
  TGTACTTAAAGTGGCGGTAGATGGTAATGTAATAAGGCAAGTGTTGGAGAATAATCAAGCCAATCTTGCA
  TCCAAAGAACAAGAAACAATCAATGCTTCACCCATACAACAAGGTGGCCATTCTGTTATTTCAGCCATCA
  GTCTTCCTTTGGTTGATCAAGATGGAACAACCAAAATTATCATCAACTACAGTCTTGAGCAGCCTAGCCA
  ACTTCAAGTTGTTCCTCAAAATTTAAAAAAAGAAAATCCAGTCGCTACAAACAGTTGTAAAAGTGAAAAG
  TTACCAGAAGATCTTACTGTTAAGTCTGAGAAGGACAAAAGCTTTGAAGGGGGGGTGAATGATAGCACTT
  GTCTTCTGTGTGATGATTGTCCAGGAGATATTAATGCACTTCCAGAATTAAAGCACTATGACCTAAAGCA
  GCCTACTCAGCCTCCTCCACTCCCTGCAGCAGAAGCTGAGAAGCCTGAGTCCTCTGTTTCATCAGCTACT
  GGAGATGGCAATTTGTCTCCTAGTCAGCCACCTTTAAAGAACCTCTTGTCTCTCCTAAAAGCATATTATG
  CTTTGAATGCACAACCAAGTGCAGAAGAGCTCTCAAAAATTGCTGATTCAGTAAACCTACCACTGGATGT
  AGTAAAAAAGTGGTTTGAAAAGATGCAAGCTGGACAGATTTCAGTGCAGTCTTCTGAACCATCTTCTCCT
  GAACCAGGCAAAGTAAATATCCCTGCCAAGAACAATGATCAGCCTCAATCTGCAAATGCAAATGAACCCC
  AGGACAGCACAGTAAATCTACAAAGTCCTTTGAAGATGACTAACTCCCCAGTTTTACCAGTGGGATCAAC
  CACCAATGGTTCCAGAAGTAGTACACCATCCCCATCACCTCTAAACCTTTCCTCATCCAGAAATACACAG
  GGTTACTTGTACACAGCTGAGGGTGCACAAGAAGAGCCACAAGTAGAACCTCTTGATCTTTCACTACCAA
  AGCAACAGGGAGAATTATTAGAAAGGTCAACTATCACTAGTGTTTACCAGAACAGTGTTTATTCTGTCCA
  GGAAGAACCCTTGAACTTGTCTTGCGCAAAAAAGGAGCCACAAAAGGACAGTTGTGTTACAGACTCAGAA
  CCAGTTGTAAATGTAATCCCACCAAGTGCCAACCCCATAAATATCGCTATACCTACAGTCACTGCCCAGT
  TACCCACAATCGTGGCCATTGCTGACCAGAACAGTGTTCCATGCTTAAGAGCGCTAGCTGCCAATAAGCA
  AACGATTCTGATTCCCCAGGTGGCATACACCTACTCAACTACGGTCAGCCCTGCAGTCCAAGAACCACCC
  TTGAAAGTGATCCAGCCAAATGGAAATCAGGATGAAAGACAAGATACTAGCTCAGAAGGAGTATCAAATG
  TAGAGGATCAGAATGACTCTGATTCTACACCGCCCAAAAAGAAAATGCGGAAGACAGAAAATGGAATGTA
  TGCTTGTGATTTGTGTGACAAGATATTCCAAAAGAGTAGTTCATTATTGAGACATAAATATGAACACACA
  GGTAAAAGACCTCATGAGTGTGGAATCTGTAAAAAGGCATTTAAACACAAACATCATTTGATTGAACACA
  TGCGATTACATTCTGGAGAAAAGCCCTATCAATGTGACAAATGTGGAAAGCGCTTCTCACACTCTGGGTC
  TTATTCTCAACACATGAATCATCGCTACTCCTACTGTAAGAGAGAAGCGGAAGAACGTGACAGCACAGAG
  CAGGAAGAGGCAGGGCCTGAAATCCTCTCGAATGAGCACGTGGGTGCCAGGGCGTCTCCCTCACAGGGCG
  ACTCGGACGAGAGAGAGAGTTTGACAAGGGAAGAGGATGAAGACAGTGAAAAAGAGGAAGAGGAGGAGGA
  TAAAGAGATGGAAGAATTGCAGGAAGAAAAAGAATGTGAAAAACCACAAGGGGATGAGGAAGAGGAGGAG
  GAGGAGGAAGAAGTGGAAGAAGAAGAGGTAGAAGAGGCAGAGAATGAGGGAGAAGAAGCAAAAACTGAAG
  GTCTGATGAAGGATGACAGGGCTGAAAGTCAAGCAAGCAGCTTAGGACAAAAAGTAGGCGAGAGTAGTGA
  GCAAGTGTCTGAAGAAAAGACAAATGAAGCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG230651 representing NM_001174094
  Red=Cloning site Green=Tags(s)
  
  MKVTNYNTVVETNSDSDDEDKLHIVEEESVTDAADCEGVPEDDLPTDQTVLPGRSSEREGNAKNCWEDDR
  KEGQEILGPEAQADEAGCTVKDDECESDAENEQNHDPNVEEFLQQQDTAVIFPEAPEEDQRQGTPEASGH
  DENGTPDAFSQLLTCPYCDRGYKRFTSLKEHIKYRHEKNEDNFSCSLCSYTFAYRTQLERHMTSHKSGRD
  QRHVTQSGCNRKFKCTECGKAFKYKHHLKEHLRIHSGEKPYECPNCKKRFSHSGSYSSHISSKKCISLIP
  VNGRPRTGLKTSQCSSPSLSASPGSPTRPQIRQKIENKPLQEQLSVNQIKTEPVDYEFKPIVVASGINCS
  TPLQNGVFTGGGPLQATSSPQGMVQAVVLPTVGLVSPISINLSDIQNVLKVAVDGNVIRQVLENNQANLA
  SKEQETINASPIQQGGHSVISAISLPLVDQDGTTKIIINYSLEQPSQLQVVPQNLKKENPVATNSCKSEK
  LPEDLTVKSEKDKSFEGGVNDSTCLLCDDCPGDINALPELKHYDLKQPTQPPPLPAAEAEKPESSVSSAT
  GDGNLSPSQPPLKNLLSLLKAYYALNAQPSAEELSKIADSVNLPLDVVKKWFEKMQAGQISVQSSEPSSP
  EPGKVNIPAKNNDQPQSANANEPQDSTVNLQSPLKMTNSPVLPVGSTTNGSRSSTPSPSPLNLSSSRNTQ
  GYLYTAEGAQEEPQVEPLDLSLPKQQGELLERSTITSVYQNSVYSVQEEPLNLSCAKKEPQKDSCVTDSE
  PVVNVIPPSANPINIAIPTVTAQLPTIVAIADQNSVPCLRALAANKQTILIPQVAYTYSTTVSPAVQEPP
  LKVIQPNGNQDERQDTSSEGVSNVEDQNDSDSTPPKKKMRKTENGMYACDLCDKIFQKSSSLLRHKYEHT
  GKRPHECGICKKAFKHKHHLIEHMRLHSGEKPYQCDKCGKRFSHSGSYSQHMNHRYSYCKREAEERDSTE
  QEEAGPEILSNEHVGARASPSQGDSDERESLTREEDEDSEKEEEEEDKEMEELQEEKECEKPQGDEEEEE
  EEEEVEEEEVEEAENEGEEAKTEGLMKDDRAESQASSLGQKVGESSEQVSEEKTNEA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001174094
• ORF Size: 3321 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001174094.2
• RefSeq Size: 6275 bp
• RefSeq ORF: 3324 bp
• Locus ID: 6935
• UniProt ID: P37275
• Cytogenetics: 10p11.22
• Protein Families: Transcription Factors
• Gene Summary: This gene encodes a zinc finger transcription factor. The encoded protein likely plays a role in transcriptional repression of interleukin 2. Mutations in this gene have been associated with posterior polymorphous corneal dystrophy-3 and late-onset Fuchs endothelial corneal dystrophy. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Mar 2010]