GDPD2 (NM_001171193) Human Tagged ORF Clone

CAT#: RG230121

GDPD2 (tGFP-tagged) - Human glycerophosphodiester phosphodiesterase domain containing 2 (GDPD2), transcript variant 4

Product Images

Circular map for RG230121

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: GDPD2
• Synonyms: GDE3; OBDPF
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG230121 representing NM_001171193
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTGGTCCCTGGCATTCCTGCTGGTCATCTCTCTACTGGTCACATATGCATCCTTGCTATTGGTCC
  TGGCCCTGCTCCTGCGGCTTTGTAGACAGCCCCTGCATCTGCACAGCCTCCACAAGGTGCTGCTGCTCCT
  CATTATGCTGCTTGTGGCGGCTGGCCTTGTGGGACTGGACATCCAATGGCAGCAGGAGTGGCATAGCTTG
  CGTGTGTCACTGCAGGCCACAGCCCCATTCCTTCATATTGGAGCAGCCGCTGGAATTGCCCTCCTGGCCT
  GGCCTGTGGCTGATACCTTCTACCGTATCCACCGAAGAGGTCCCAAGATTCTGCTACTGCTCCTATTTTT
  TGGAGTTGTCCTGGTCATCTACTTGGCCCCCCTATGCATCTCCTCACCCTGCATCATGGAACCCAGAGAC
  TTACCACCCAAGCCTGGGCTGGTGGGACACCGAGGGGCCCCCATGCTGGCTCCCGAGAACACCCTGATGT
  CCTTGCGGAAGACAGCTGAATGCGGAGCTACTGTGTTTGAGACTGATGTGATGGTCAGCTCCGATGGGGT
  CCCCTTCCTCATGCATGATGAGCACCTCAGCAGGACCACGAATGTAGCCTCTGTATTCCCAACCCGAATC
  ACAGCCCACAGCAGTGACTTCTCCTGGACTGAACTGAAGAGACTCAATGCTGGATCCTGGTTCCTAGAGA
  GGCGACCCTTCTGGGGGGCCAAACCGCTGGCAGGCCCTGATCAGAAAGAGGCTGAGAGTCAGACGGTACC
  AGCATTAGAAGAGCTATTGGAGGAAGCTGCAGCCCTCAACCTTTCCATCATGTTCGACTTGCGCCGACCC
  CCACAGAACCACACATACTATGACACTTTTGTGATCCAGACATTGGAGACTGTGCTGAATGCAAGGGTGC
  CCCAAGCCATGGTCTTTTGGCTACCAGATGAAGATCGGGCTAATGTCCAACGACGGGCACCTGGAATGCG
  CCAGATATATGGACGTCAGGGAGGCAACAGAACGGAGAGGCCCCAGTTTCTTAACCTCCCCTATCAAGAT
  CTGCCACTATTGGATATCAAGGCATTGCATAAGGATAATGTCTCGGTGAACCTATTTGTAGTGAACAAGC
  CCTGGCTCTTCTCTCTGCTTTGGTGTGCAGGGGTGGATTCGGTCACCACCAACGACTGCCAGCTGCTGCA
  GCAGATGCGTTACCCTATCTGGCTTATTACCCCTCAAACCTACCTAATCATATGGGTCATTACCAATTGT
  GTTTCCACCATGCTGCTTTTGTGGACCTTCCTCCTCCAAAGAAGATTTGTTAAGAAGAGAGGGAAAACTG
  GCTTAGAAACAGCAGTGCTGCTGACAAGGATCAACAATTTCATGATGGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG230121 representing NM_001171193
  Red=Cloning site Green=Tags(s)
  
  MDWSLAFLLVISLLVTYASLLLVLALLLRLCRQPLHLHSLHKVLLLLIMLLVAAGLVGLDIQWQQEWHSL
  RVSLQATAPFLHIGAAAGIALLAWPVADTFYRIHRRGPKILLLLLFFGVVLVIYLAPLCISSPCIMEPRD
  LPPKPGLVGHRGAPMLAPENTLMSLRKTAECGATVFETDVMVSSDGVPFLMHDEHLSRTTNVASVFPTRI
  TAHSSDFSWTELKRLNAGSWFLERRPFWGAKPLAGPDQKEAESQTVPALEELLEEAAALNLSIMFDLRRP
  PQNHTYYDTFVIQTLETVLNARVPQAMVFWLPDEDRANVQRRAPGMRQIYGRQGGNRTERPQFLNLPYQD
  LPLLDIKALHKDNVSVNLFVVNKPWLFSLLWCAGVDSVTTNDCQLLQQMRYPIWLITPQTYLIIWVITNC
  VSTMLLLWTFLLQRRFVKKRGKTGLETAVLLTRINNFMME
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001171193
• ORF Size: 1380 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001171193.1, NP_001164664.1
• RefSeq Size: 2176 bp
• RefSeq ORF: 1383 bp
• Locus ID: 54857
• UniProt ID: Q9HCC8
• Cytogenetics: Xq13.1
• Protein Families: Transmembrane
• Gene Summary: This gene encodes a member of the glycerophosphodiester phosphodiesterase enzyme family. The encoded protein hydrolyzes glycerophosphoinositol to produce inositol 1-phosphate and glycerol. This protein may have a role in osteoblast differentiation and growth. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2010]