Repulsive Guidance Molecule A (RGMA) (NM_001166286) Human Tagged ORF Clone

CAT#: RG230079

RGMA (tGFP-tagged) - Human RGM domain family, member A (RGMA), transcript variant 2

Product Images

Circular map for RG230079

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Repulsive Guidance Molecule A
• Synonyms: RGM
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG230079 representing NM_001166286
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTATGGGGAGAGGGGCAGGACGTTCAGCCCTGGGATTCTGGCCGACCCTCGCCTTCCTTCTCTGCA
  GCTTCCCCGCAGCCACCTCCCCGTGCAAGATCCTCAAGTGCAACTCTGAGTTCTGGAGCGCCACGTCGGG
  CAGCCACGCCCCAGCCTCAGACGACACCCCCGAGTTCTGTGCAGCCTTGCGCAGCTACGCCCTGTGCACG
  CGGCGGACGGCCCGCACCTGCCGGGGTGACCTGGCCTACCACTCGGCCGTCCATGGCATAGAGGACCTCA
  TGAGCCAGCACAACTGCTCCAAGGATGGCCCCACCTCGCAGCCACGCCTGCGCACGCTCCCACCGGCCGG
  AGACAGCCAGGAGCGCTCGGACAGCCCCGAGATCTGCCATTACGAGAAGAGCTTTCACAAGCACTCGGCC
  ACCCCCAACTACACGCACTGTGGCCTCTTCGGGGACCCACACCTCAGGACTTTCACCGACCGCTTCCAGA
  CCTGCAAGGTGCAGGGCGCCTGGCCGCTCATCGACAATAATTACCTGAACGTGCAGGTCACCAACACGCC
  TGTGCTGCCCGGCTCAGCGGCCACTGCCACCAGCAAGCTCACCATCATCTTCAAGAACTTCCAGGAGTGT
  GTGGACCAGAAGGTGTACCAGGCTGAGATGGACGAGCTCCCGGCCGCCTTCGTGGATGGCTCTAAGAACG
  GTGGGGACAAGCACGGGGCCAACAGCCTGAAGATCACTGAGAAGGTGTCAGGCCAGCACGTGGAGATCCA
  GGCCAAGTACATCGGCACCACCATCGTGGTGCGCCAGGTGGGCCGCTACCTGACCTTTGCCGTCCGCATG
  CCAGAGGAAGTGGTCAATGCTGTGGAGGACTGGGACAGCCAGGGTCTCTACCTCTGCCTGCGGGGCTGCC
  CCCTCAACCAGCAGATCGACTTCCAGGCCTTCCACACCAATGCTGAGGGCACCGGTGCCCGCAGGCTGGC
  AGCCGCCAGCCCTGCACCCACAGCCCCCGAGACCTTCCCATACGAGACAGCCGTGGCCAAGTGCAAGGAG
  AAGCTGCCGGTGGAGGACCTGTACTACCAGGCCTGCGTCTTCGACCTCCTCACCACGGGCGACGTGAACT
  TCACACTGGCCGCCTACTACGCGTTGGAGGATGTCAAGATGCTCCACTCCAACAAAGACAAACTGCACCT
  GTATGAGAGGACTCGGGACCTGCCAGGCAGGGCGGCTGCGGGGCTGCCCCTGGCCCCCCGGCCCCTCCTG
  GGCGCCCTCGTCCCGCTCCTGGCCCTGCTCCCTGTGTTCTGC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG230079 representing NM_001166286
  Red=Cloning site Green=Tags(s)
  
  MGMGRGAGRSALGFWPTLAFLLCSFPAATSPCKILKCNSEFWSATSGSHAPASDDTPEFCAALRSYALCT
  RRTARTCRGDLAYHSAVHGIEDLMSQHNCSKDGPTSQPRLRTLPPAGDSQERSDSPEICHYEKSFHKHSA
  TPNYTHCGLFGDPHLRTFTDRFQTCKVQGAWPLIDNNYLNVQVTNTPVLPGSAATATSKLTIIFKNFQEC
  VDQKVYQAEMDELPAAFVDGSKNGGDKHGANSLKITEKVSGQHVEIQAKYIGTTIVVRQVGRYLTFAVRM
  PEEVVNAVEDWDSQGLYLCLRGCPLNQQIDFQAFHTNAEGTGARRLAAASPAPTAPETFPYETAVAKCKE
  KLPVEDLYYQACVFDLLTTGDVNFTLAAYYALEDVKMLHSNKDKLHLYERTRDLPGRAAAGLPLAPRPLL
  GALVPLLALLPVFC
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001166286
• ORF Size: 1302 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001166286.2
• RefSeq Size: 3060 bp
• RefSeq ORF: 1305 bp
• Locus ID: 56963
• UniProt ID: Q96B86
• Cytogenetics: 15q26.1
• Gene Summary: This gene encodes a member of the repulsive guidance molecule family. The encoded protein is a glycosylphosphatidylinositol-anchored glycoprotein that functions as an axon guidance protein in the developing and adult central nervous system. This protein may also function as a tumor suppressor in some cancers. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]