UDP glucose dehydrogenase (UGDH) (NM_001184701) Human Tagged ORF Clone

CAT#: RG229996

UGDH (tGFP-tagged) - Human UDP-glucose 6-dehydrogenase (UGDH), transcript variant 3

Product Images

Circular map for RG229996

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: UDP glucose dehydrogenase
• Synonyms: DEE84; EIEE84; GDH; UDP-GlcDH; UDPGDH; UGD
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG229996 representing NM_001184701
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGAAAGGCCGGGCAGCAGATCTGAAGTATATTGAAGCTTGTGCTAGACGCATTGTGCAAAACTCAA
  ATGGGTACAAAATTGTGACTGAGAAAAGCACAGTTCCAGTGCGGGCAGCAGAAAGTATCCGTCGCATATT
  TGATGCAAACACAAAACCCAACTTGAATTTACAGGTGCTGTCCAACCCTGAGTTTCTGGCAGAGGGAACA
  GCCATCAAGGACCTAAAGAACCCAGACAGAGTACTGATTGGAGGGGATGAAACTCCAGAGGGCCAGAGAG
  CTGTGCAGGCCCTGTGTGCTGTATATGAGCACTGGGTTCCCAGAGAAAAGATCCTCACCACTAATACTTG
  GTCTTCAGAGCTTTCCAAACTGGCAGCAAATGCTTTTCTTGCCCAGAGAATAAGCAGCATTAACTCCATA
  AGTGCTCTGTGTGAAGCAACAGGAGCTGATGTAGAAGAGGTAGCAACAGCGATTGGAATGGACCAGAGAA
  TTGGAAACAAGTTTCTAAAAGCCAGTGTTGGGTTTGGTGGGAGCTGTTTCCAAAAGGATGTTCTGAATTT
  GGTTTATCTCTGTGAGGCTCTGAATTTGCCAGAAGTAGCTCGTTATTGGCAGCAGGTCATAGACATGAAT
  GACTACCAGAGGAGGAGGTTTGCTTCCCGGATCATAGATAGTCTGTTTAATACAGTAACTGATAAGAAGA
  TAGCTATTTTGGGATTTGCATTCAAAAAGGACACTGGTGATACAAGAGAATCTTCTAGTATATATATTAG
  CAAATATTTGATGGATGAAGGTGCACATCTACATATATATGATCCAAAAGTACCTAGGGAACAAATAGTT
  GTGGATCTTTCTCATCCAGGTGTTTCAGAGGATGACCAAGTGTCCCGGCTCGTGACCATTTCCAAGGATC
  CATATGAAGCATGTGATGGTGCCCATGCTGTTGTTATTTGCACTGAGTGGGACATGTTTAAGGAATTGGA
  TTATGAACGCATTCATAAAAAAATGCTAAAGCCAGCCTTTATCTTCGATGGACGGCGTGTCCTGGATGGG
  CTCCACAATGAACTACAAACCATTGGCTTCCAGATTGAAACAATTGGCAAAAAGGTGTCTTCAAAGAGAA
  TTCCATATGCTCCTTCTGGTGAAATTCCGAAGTTTAGTCTTCAAGATCCACCTAACAAGAAACCTAAAGT
  G
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG229996 representing NM_001184701
  Red=Cloning site Green=Tags(s)
  
  MGKGRAADLKYIEACARRIVQNSNGYKIVTEKSTVPVRAAESIRRIFDANTKPNLNLQVLSNPEFLAEGT
  AIKDLKNPDRVLIGGDETPEGQRAVQALCAVYEHWVPREKILTTNTWSSELSKLAANAFLAQRISSINSI
  SALCEATGADVEEVATAIGMDQRIGNKFLKASVGFGGSCFQKDVLNLVYLCEALNLPEVARYWQQVIDMN
  DYQRRRFASRIIDSLFNTVTDKKIAILGFAFKKDTGDTRESSSIYISKYLMDEGAHLHIYDPKVPREQIV
  VDLSHPGVSEDDQVSRLVTISKDPYEACDGAHAVVICTEWDMFKELDYERIHKKMLKPAFIFDGRRVLDG
  LHNELQTIGFQIETIGKKVSSKRIPYAPSGEIPKFSLQDPPNKKPKV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001184701
• ORF Size: 1191 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001184701.2
• RefSeq Size: 3026 bp
• RefSeq ORF: 1194 bp
• Locus ID: 7358
• UniProt ID: O60701
• Cytogenetics: 4p14
• Protein Pathways: Amino sugar and nucleotide sugar metabolism, Ascorbate and aldarate metabolism, Metabolic pathways, Pentose and glucuronate interconversions, Starch and sucrose metabolism
• Gene Summary: The protein encoded by this gene converts UDP-glucose to UDP-glucuronate and thereby participates in the biosynthesis of glycosaminoglycans such as hyaluronan, chondroitin sulfate, and heparan sulfate. These glycosylated compounds are common components of the extracellular matrix and likely play roles in signal transduction, cell migration, and cancer growth and metastasis. The expression of this gene is up-regulated by transforming growth factor beta and down-regulated by hypoxia. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]