SORBS2 (NM_003603) Human Tagged ORF Clone

CAT#: RG229329

SORBS2 (tGFP-tagged) - Human sorbin and SH3 domain containing 2 (SORBS2), transcript variant 1

Product Images

Circular map for RG229329

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SORBS2
• Synonyms: ARGBP2; PRO0618
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG229329 representing NM_003603
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACACAGGGCGTGATTCTCAGTCACCAGACTCAGCAAAAGGTTTTAGAAGCGTTCGACCAAACCTAC
  AAGATAAAAGATCACCAACTCAGAGCCAGATAACAGTGAATGGAAACTCAGGAGGTGCCGTGAGTCCCAT
  GAGTTACTATCAGAGGCCGTTTTCCCCCTCGGCATATTCTCTCCCAGCCTCACTCAACTCCAGCATTGTC
  ATGCAGCACGGCACATCCCTCGATTCCACAGACACATATCCCCAGCATGCGCAGTCTCTGGATGGCACCA
  CCAGCAGCTCTATCCCCCTGTACCGATCCTCAGAGGAAGAGAAGAGAGTGACAGTCATCAAAGCCCCGCA
  TTACCCAGGGATCGGGCCCGTGGATGAATCCGGAATCCCCACAGCAATTAGAACGACAGTCGACCGGCCC
  AAGGACTGGTACAAGACGATGTTTAAGCAAATTCACATGGTGCACAAGCCGGATGATGACACAGACATGT
  ATAATACTCCTTATACATACAATGCAGGTCTGTACAACCCACCCTACAGTGCTCAGTCACACCCTGCTGC
  AAAGACCCAAACCTACAGACCTCTTTCCAAAAGCCACTCCGACAACAGCCCCAATGCCTTTAAGGATGCG
  TCCTCCCCAGTGCCTCCCCCACATGTTCCACCTCCAGTCCCGCCGCTTCGACCAAGAGATCGGTCTTCAA
  CAGAAAAGCATGACTGGGATCCTCCAGACAGAAAAGTGGACACAAGAAAATTTCGGTCTGAGCCAAGGAG
  TATTTTTGAATATGAACCTGGCAAGTCATCAATTCTTCAGCATGAAAGACCAACTGATCGCATAAATCCA
  GATGACATAGATTTAGAAAATGAGCCCTGGTATAAATTCTTTTCAGAACTGGAGTTTGGACGCCCGCCTC
  CTAAAAAGCCTCTGGACTATGTTCAAGATCATTCTTCTGGTGTTTTCAATGAGGCCTCCTTGTATCAGTC
  CTCTATAGACAGAAGCCTGGAAAGACCCATGAGTTCTGCAAGCATGGCCAGTGACTTCAGGAAGCGGAGG
  AAGAGCGAGCCTGCAGTGGGTCCACCACGGGGCTTGGGAGATCAAAGTGCGAGCAGGACTAGCCCAGGCC
  GAGTGGACCTCCCAGGATCAAGCACCACTCTTACAAAGTCTTTCACTAGCTCTTCTCCTTCTTCCCCATC
  AAGAGCAAAAGACCGTGAGTCCCCTAGAAGTTACTCATCCACTTTGACTGACATGGGGAGAAGTGCACCA
  AGGGAAAGAAGAGGAACTCCAGAAAAAGAGAAATTGCCTGCAAAAGCTGTTTATGATTTTAAGGCTCAGA
  CATCTAAGGAGTTGTCATTTAAGAAAGGAGATACTGTCTACATCCTCAGGAAAATTGATCAAAATTGGTA
  TGAGGGAGAACACCACGGGAGAGTGGGCATCTTCCCGATCTCATACGTAGAGAAACTCACACCTCCTGAG
  AAAGCACAGCCTGCAAGACCACCTCCGCCAGCCCAGCCCGGAGAAATCGGAGAAGCTATAGCCAAATACA
  ACTTCAACGCAGACACAAATGTGGAGCTGTCACTGAGAAAGGGAGATAGAGTTATTCTTCTTAAAAGAGT
  TGATCAAAACTGGTATGAAGGTAAAATCCCAGGAACCAACAGACAAGGCATCTTCCCTGTTTCCTATGTG
  GAGGTCGTCAAGAAGAACACAAAAGGTGCTGAGGACTACCCTGACCCTCCAATACCCCACAGCTATTCTA
  GTGATAGGATTCACAGCTTGAGCTCAAATAAGCCACAGCGTCCTGTGTTTACTCATGAAAATATTCAAGG
  TGGGGGGGAACCGTTTCAGGCTCTGTATAACTATACTCCCAGGAATGAAGATGAGCTGGAGCTCAGAGAA
  AGTGATGTCATTGATGTCATGGAAAAGTGTGATGACGGCTGGTTTGTGGGGACCTCAAGAAGAACCAAAT
  TCTTTGGTACTTTCCCCGGAAACTACGTCAAGAGGCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG229329 representing NM_003603
  Red=Cloning site Green=Tags(s)
  
  MNTGRDSQSPDSAKGFRSVRPNLQDKRSPTQSQITVNGNSGGAVSPMSYYQRPFSPSAYSLPASLNSSIV
  MQHGTSLDSTDTYPQHAQSLDGTTSSSIPLYRSSEEEKRVTVIKAPHYPGIGPVDESGIPTAIRTTVDRP
  KDWYKTMFKQIHMVHKPDDDTDMYNTPYTYNAGLYNPPYSAQSHPAAKTQTYRPLSKSHSDNSPNAFKDA
  SSPVPPPHVPPPVPPLRPRDRSSTEKHDWDPPDRKVDTRKFRSEPRSIFEYEPGKSSILQHERPTDRINP
  DDIDLENEPWYKFFSELEFGRPPPKKPLDYVQDHSSGVFNEASLYQSSIDRSLERPMSSASMASDFRKRR
  KSEPAVGPPRGLGDQSASRTSPGRVDLPGSSTTLTKSFTSSSPSSPSRAKDRESPRSYSSTLTDMGRSAP
  RERRGTPEKEKLPAKAVYDFKAQTSKELSFKKGDTVYILRKIDQNWYEGEHHGRVGIFPISYVEKLTPPE
  KAQPARPPPPAQPGEIGEAIAKYNFNADTNVELSLRKGDRVILLKRVDQNWYEGKIPGTNRQGIFPVSYV
  EVVKKNTKGAEDYPDPPIPHSYSSDRIHSLSSNKPQRPVFTHENIQGGGEPFQALYNYTPRNEDELELRE
  SDVIDVMEKCDDGWFVGTSRRTKFFGTFPGNYVKRL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003603
• ORF Size: 1998 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003603.6, NP_003594.3
• RefSeq Size: 3360 bp
• RefSeq ORF: 2001 bp
• Locus ID: 8470
• UniProt ID: O94875
• Cytogenetics: 4q35.1
• Domains: SH3, Sorb
• Gene Summary: Arg and c-Abl represent the mammalian members of the Abelson family of non-receptor protein-tyrosine kinases. They interact with the Arg/Abl binding proteins via the SH3 domains present in the carboxy end of the latter group of proteins. This gene encodes the sorbin and SH3 domain containing 2 protein. It has three C-terminal SH3 domains and an N-terminal sorbin homology (SoHo) domain that interacts with lipid raft proteins. The subcellular localization of this protein in epithelial and cardiac muscle cells suggests that it functions as an adapter protein to assemble signaling complexes in stress fibers, and that it is a potential link between Abl family kinases and the actin cytoskeleton. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]