PPM1M (NM_144641) Human Tagged ORF Clone

CAT#: RG229147

PPM1M (tGFP-tagged) - Human protein phosphatase, Mg2+/Mn2+ dependent, 1M (PPM1M), transcript variant 1

Product Images

Circular map for RG229147

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PPM1M
• Synonyms: PP2C-eta; PP2CE; PP2Ceta
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG229147 representing NM_144641
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACCTCAATGGCCGCTGCATCTGCCCCAGTGACCCTCAGTTTGTGGAGGAAAAGGGCATCAGGGCAG
  AAGACTTGGTGATCGGGGCATTGGAGAGTGCCTTTCAGGAATGTGATGAGGTGATCGGGCGGGAGCTGGA
  GGCCTCAGGCCAGATGGGCGGCTGCACAGCCCTGGTGGCTGTGTCCCTGCAGGGAAAGCTGTACATGGCC
  AATGCTGGGGATAGCAGGGCCATCTTGGTGCGGAGAGATGAGATACGGCCACTGAGCTTCGAGTTCACCC
  CAGAGACTGAGCGGCAGCGGATCCAGCAGCTGGCCTTTGTCTATCCTGAGCTTCTGGCTGGTGAGTTCAC
  CCGACTGGAGTTCCCTCGGCGGCTGAAGGGGGATGACTTGGGACAGAAGGTTTTGTTCAGGGATCACCAC
  ATGAGTGGCTGGAGCTACAAACGTGTGGAGAAATCGGATCTCAAGTACCCACTGATCCATGGACAGGGTA
  GGCAGGCTCGGTTACTAGGAACACTGGCTGTCTCCCGGGGCCTGGGAGACCATCAGCTCAGAGTCCTGGA
  CACAAACATCCAGCTCAAGCCCTTCTTGCTCTCTGTGCCACAGGTGACTGTGCTGGATGTGGACCAGCTG
  GAGCTACAGGAGGATGATGTGGTTGTCATGGCAACTGATGGACTCTGGGATGTACTGTCCAACGAGCAGG
  TGGCATGGCTGGTGCGGAGCTTCCTCCCTGGGAACCAAGAGGACCCACACAGGTTCTCAAAGCTGGCCCA
  GATGCTGATACACAGCACACAGGGAAAGGAAGACAGTCTCACAGAGGAAGGGCAGGTGTCCTACGATGAC
  GTCTCTGTGTTCGTGATTCCCTTGCACAGTCAGGGCCAAGAGAGCAGTGACCAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG229147 representing NM_144641
  Red=Cloning site Green=Tags(s)
  
  MHLNGRCICPSDPQFVEEKGIRAEDLVIGALESAFQECDEVIGRELEASGQMGGCTALVAVSLQGKLYMA
  NAGDSRAILVRRDEIRPLSFEFTPETERQRIQQLAFVYPELLAGEFTRLEFPRRLKGDDLGQKVLFRDHH
  MSGWSYKRVEKSDLKYPLIHGQGRQARLLGTLAVSRGLGDHQLRVLDTNIQLKPFLLSVPQVTVLDVDQL
  ELQEDDVVVMATDGLWDVLSNEQVAWLVRSFLPGNQEDPHRFSKLAQMLIHSTQGKEDSLTEEGQVSYDD
  VSVFVIPLHSQGQESSDH
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144641
• ORF Size: 894 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144641.2, NP_653242.2
• RefSeq Size: 2054 bp
• RefSeq ORF: 1380 bp
• Locus ID: 132160
• UniProt ID: Q96MI6
• Cytogenetics: 3p21.2
• Domains: PP2C
• Protein Families: Druggable Genome, Phosphatase