CASS4 (NM_001164116) Human Tagged ORF Clone

CAT#: RG228580

CASS4 (tGFP-tagged) - Human Cas scaffolding protein family member 4 (CASS4), transcript variant 1

Product Images

Circular map for RG228580

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CASS4
• Synonyms: C20orf32; CAS4; HEFL; HEPL
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG228580 representing NM_001164116
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGGAACAGGCATCATGGACTGTGCGCCCAAGGCACTCCTGGCCAGGGCACTTTATGACAACTGCC
  CTGACTGCTCTGACGAGCTGGCTTTCAGCAGAGGGGACATCCTGACCATTCTGGAGCAACACGTGCCAGA
  AAGCGAGGGTTGGTGGAAGTGTTTGCTCCATGGGAGGCAAGGCCTGGCCCCTGCCAACCGCCTCCAAATC
  CTCACGGAGGTCGCTGCAGACAGGCCGTGCCCCCCATTCCTGAGAGGCCTGGAAGAAGCTCCTGCCAGCT
  CAGAGGAGACCTATCAGGTGCCCACTCTACCCCGCCCTCCCACTCCAGGCCCCGTTTATGAGCAGATGAG
  GAGTTGGGCGGAGGGGCCCCAGCCCCCTACTGCCCAAGTCTATGAATTCCCCGACCCTCCCACCAGTGCC
  AGAATCATCTGTGAAAAGACTCTCAGCTTTCCAAAACAGGCCATCCTCACGCTTCCCAGACCTGTCCGGG
  CCTCACTGCCGACTCTGCCTTCCCAGGTGTATGACGTGCCTACCCAGCACCGGGGCCCCGTGGTCCTGAA
  GGAGCCAGAGAAGCAGCAGTTATATGACATACCAGCCAGCCCCAAGAAGGCAGGACTCCATCCCCCAGAC
  AGCCAAGCAAGTGGGCAGGGTGTTCCCCTGATATCAGTGACTACCTTAAGAAGAGGCGGTTACAGCACAT
  TACCAAATCCTCAGAAATCGGAATGGATTTATGACACTCCAGTGTCTCCAGGAAAGGCCAGCGTCAGAAA
  CACGCCTCTCACCAGCTTTGCGGAAGAATCAAGGCCCCACGCTCTCCCCAGTTCCAGCTCCACTTTCTAC
  AATCCTCCAAGTGGCAGATCCAGGTCCCTCACTCCACAACTGAATAACAATGTGCCCATGCAGAAAAAAC
  TCAGCCTTCCAGAAATTCCTTCTTATGGCTTTCTTGTACCCAGAGGCACATTTCCTTTGGATGAAGATGT
  CAGCTACAAGGTTCCTTCAAGCTTTCTGATTCCCCGAGTGGAACAGCAGAACACCAAGCCCAATATTTAT
  GACATCCCTAAAGCAACGTCGAGTGTTTCTCAGGCTGGGAAGGAGCTGGAGAAAGCCAAGGAGGTGTCAG
  AGAATTCCGCGGGCCATAATTCCTCATGGTTCTCCAGACGGACAACTTCCCCATCTCCTGAACCGGACAG
  ATTATCAGGTTCCAGTTCTGACAGCAGAGCTAGCATCGTTTCCTCGTGCTCCACCACATCCACCGACGAC
  TCCTCCAGCTCTTCCTCGGAGGAGTCAGCAAAGGAGCTCTCCTTGGACCTGGATGTGGCCAAGGAGACAG
  TGATGGCTCTGCAGCACAAGGTGGTCAGCTCTGTCGCTGGCCTGATGCTCTTTGTCAGCAGGAAGTGGAG
  ATTCCGAGACTATCTGGAGGCCAACATTGATGCAATCCACAGGTCCACTGATCACATAGAAGAATCTGTA
  AGAGAATTTCTGGATTTTGCCCGAGGAGTCCATGGGACTGCCTGTAACCTCACTGACAGTAACCTTCAGA
  ACAGAATTCGGGACCAGATGCAGACCATCTCCAACTCCTACCGCATCCTGCTTGAAACAAAGGAAAGCTT
  GGATAATCGCAATTGGCCTCTGGAAGTTCTTGTGACTGACAGTGTCCAGAACAGCCCAGATGACCTTGAG
  AGGTTTGTCATGGTGGCACGGATGCTTCCAGAAGACATCAAGAGGTTTGCCTCCATTGTCATTGCCAATG
  GAAGGCTCCTTTTTAAGCGGAACTGTGAAAAGGAAGAGACTGTGCAGTTGACCCCAAATGCAGAATTTAA
  GTGTGAAAAATACATCCAGCCTCCCCAAAGAGAAACTGAATCACACCAAAAGAGTACCCCTTCCACTAAG
  CAAAGGGAAGATGAACACTCTTCTGAACTATTAAAGAAAAATAGGGCAAATATCTGTGGACAGAATCCTG
  GCCCTCTTATACCTCAGCCTTCGAGTCAACAGACTCCTGAGAGGAAACCCCGCTTATCTGAACACTGCCG
  GCTCTACTTTGGGGCGCTCTTCAAAGCCATCAGCGCATTTCACGGCAGCCTCAGCAGCAGCCAGCCCGCG
  GAGATCATCACTCAGAGCAAGCTGGTCATCATGGTGGGACAGAAGCTGGTGGACACGCTGTGCATGGAGA
  CCCAGGAGAGGGACGTGCGCAACGAGATCCTCCGTGGCAGCAGTCACCTCTGCAGCCTGCTCAAGGACGT
  AGCGCTGGCCACTAAGAATGCCGTGCTCACGTACCCCAGCCCTGCCGCGCTGGGGCACCTCCAGGCGGAG
  GCTGAGAAGCTGGAGCAACACACGCGGCAGTTCAGAGGGACACTGGGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG228580 representing NM_001164116
  Red=Cloning site Green=Tags(s)
  
  MKGTGIMDCAPKALLARALYDNCPDCSDELAFSRGDILTILEQHVPESEGWWKCLLHGRQGLAPANRLQI
  LTEVAADRPCPPFLRGLEEAPASSEETYQVPTLPRPPTPGPVYEQMRSWAEGPQPPTAQVYEFPDPPTSA
  RIICEKTLSFPKQAILTLPRPVRASLPTLPSQVYDVPTQHRGPVVLKEPEKQQLYDIPASPKKAGLHPPD
  SQASGQGVPLISVTTLRRGGYSTLPNPQKSEWIYDTPVSPGKASVRNTPLTSFAEESRPHALPSSSSTFY
  NPPSGRSRSLTPQLNNNVPMQKKLSLPEIPSYGFLVPRGTFPLDEDVSYKVPSSFLIPRVEQQNTKPNIY
  DIPKATSSVSQAGKELEKAKEVSENSAGHNSSWFSRRTTSPSPEPDRLSGSSSDSRASIVSSCSTTSTDD
  SSSSSSEESAKELSLDLDVAKETVMALQHKVVSSVAGLMLFVSRKWRFRDYLEANIDAIHRSTDHIEESV
  REFLDFARGVHGTACNLTDSNLQNRIRDQMQTISNSYRILLETKESLDNRNWPLEVLVTDSVQNSPDDLE
  RFVMVARMLPEDIKRFASIVIANGRLLFKRNCEKEETVQLTPNAEFKCEKYIQPPQRETESHQKSTPSTK
  QREDEHSSELLKKNRANICGQNPGPLIPQPSSQQTPERKPRLSEHCRLYFGALFKAISAFHGSLSSSQPA
  EIITQSKLVIMVGQKLVDTLCMETQERDVRNEILRGSSHLCSLLKDVALATKNAVLTYPSPAALGHLQAE
  AEKLEQHTRQFRGTLG
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001164116
• ORF Size: 2358 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001164116.2
• RefSeq Size: 3179 bp
• RefSeq ORF: 2361 bp
• Locus ID: 57091
• UniProt ID: Q9NQ75
• Cytogenetics: 20q13.31
• Gene Summary: Docking protein that plays a role in tyrosine kinase-based signaling related to cell adhesion and cell spreading. Regulates PTK2/FAK1 activity, focal adhesion integrity, and cell spreading.[UniProtKB/Swiss-Prot Function]