DISC1 (NM_001164546) Human Tagged ORF Clone

CAT#: RG228453

DISC1 (tGFP-tagged) - Human disrupted in schizophrenia 1 (DISC1), transcript variant i

Product Images

Circular map for RG228453

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DISC1
• Synonyms: C1orf136; SCZD9
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG228453 representing NM_001164546
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAGGCGGGGGTCCTCAGGGCGCCCCAGCCGCCGCCGGCGGCGGCGGCGTGAGCCACCGCGCAGGCA
  GCCGGGATTGCTTACCACCTGCAGCGTGCTTTCGGAGGCGGCGGCTGGCACGGAGGCCGGGCTACATGAG
  AAGCTCGACAGGGCCTGGGATCGGGTTCCTTTCCCCAGCAGTGGGCACACTGTTCCGGTTCCCAGGAGGG
  GTGTCTGGCGAGGAGTCCCACCACTCGGAGTCCAGGGCCAGACAGTGTGGCCTTGACTCGAGAGGCCTCT
  TGGTCCGGAGCCCTGTTTCCAAGAGTGCAGCAGCCCCTACTGTGACCTCTGTGAGAGGAACCTCGGCGCA
  CTTTGGGATTCAGCTCAGAGGTGGCACCAGATTGCCTGACAGGCTTAGCTGGCCGTGTGGCCCTGGGAGT
  GCTGGGTGGCAGCAAGAGTTTGCAGCCATGGATAGTTCTGAGACCCTGGACGCCAGCTGGGAGGCAGCCT
  GCAGCGATGGAGCAAGGCGTGTCCGGGCAGCAGGCTCTCTGCCATCAGCAGAGTTGAGTAGCAACAGCTG
  CAGCCCTGGCTGTGGCCCTGAGGTCCCCCCAACCCCTCCTGGCTCTCACAGTGCCTTTACCTCAAGCTTT
  AGCTTTATTCGGCTCTCGCTTGGCTCTGCCGGGGAACGTGGAGAAGCAGAAGGCTGCCCACCATCCAGAG
  AGGCTGAGTCCCATTGCCAGAGCCCCCAGGAGATGGGAGCCAAAGCTGCCAGCTTGGACGGGCCTCACGA
  GGACCCGCGATGTCTCTCTCGGCCCTTCAGTCTCTTGGCTACACGGGTCTCTGCAGACTTGGCCCAGGCC
  GCAAGGAACAGCTCCAGGCCAGAGCGTGACATGCATTCTTTACCAGACATGGACCCTGGCTCCTCCAGTT
  CTCTGGATCCCTCACTGGCTGGCTGTGGTGGTGATGGGAGCAGCGGCTCAGGGGATGCCCACTCTTGGGA
  CACCCTGCTCAGGAAATGGGAGCCAGTGCTGCGGGACTGCCTGCTGAGAAACCGGAGGCAGATGGAGGTA
  ATATCCTTAAGATTAAAACTTCAGAAACTTCAGGAAGATGCAGTTGAGAATGATGATTATGATAAAGCTG
  AGACGTTACAACAAAGATTAGAAGACCTGGAACAAGAGAAAATCAGCCTGCACTTTCAACTTCCTTCAAG
  GCAGCCAGCTCTTAGCAGTTTCCTGGGTCACCTGGCAGCACAAGTCCAGGCTGCCTTGCGCCGTGGGGCC
  ACTCAGCAGGCCAGCGGAGATGACACCCACACCCCACTGAGAATGGAGCCGAGGCTGTTGGAACCCACTG
  CTCAGGACAGCTTGCACGTGTCCATCACGAGACGAGACTGGCTTCTTCAGGAAAAGCAGCAGCTACAGAA
  AGAAATCGAAGCTCTCCAAGCAAGGATGTTTGTGCTGGAAGCCAAAGATCAACAGCTGAGAAGGGAAATA
  GAGGAGCAAGAGCAGCAACTCCAGTGGCAGGGCTGCGACCTGACCCCACTGGTGGGCCAGCTGTCCCTGG
  GTCAGCTGCAGGAGGTCAGCAAGGCCTTGCAGGACACCCTGGCCTCAGCCGGTCAGATTCCCTTCCATGC
  AGAGCCACCGGAAACCATAAGGAGAAACAAGTGTGAAGGAAAATACTATGAAGTACATGGAAACACT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG228453 representing NM_001164546
  Red=Cloning site Green=Tags(s)
  
  MPGGGPQGAPAAAGGGGVSHRAGSRDCLPPAACFRRRRLARRPGYMRSSTGPGIGFLSPAVGTLFRFPGG
  VSGEESHHSESRARQCGLDSRGLLVRSPVSKSAAAPTVTSVRGTSAHFGIQLRGGTRLPDRLSWPCGPGS
  AGWQQEFAAMDSSETLDASWEAACSDGARRVRAAGSLPSAELSSNSCSPGCGPEVPPTPPGSHSAFTSSF
  SFIRLSLGSAGERGEAEGCPPSREAESHCQSPQEMGAKAASLDGPHEDPRCLSRPFSLLATRVSADLAQA
  ARNSSRPERDMHSLPDMDPGSSSSLDPSLAGCGGDGSSGSGDAHSWDTLLRKWEPVLRDCLLRNRRQMEV
  ISLRLKLQKLQEDAVENDDYDKAETLQQRLEDLEQEKISLHFQLPSRQPALSSFLGHLAAQVQAALRRGA
  TQQASGDDTHTPLRMEPRLLEPTAQDSLHVSITRRDWLLQEKQQLQKEIEALQARMFVLEAKDQQLRREI
  EEQEQQLQWQGCDLTPLVGQLSLGQLQEVSKALQDTLASAGQIPFHAEPPETIRRNKCEGKYYEVHGNT
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001164546
• ORF Size: 1677 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001164546.2
• RefSeq Size: 2363 bp
• RefSeq ORF: 1680 bp
• Locus ID: 27185
• UniProt ID: Q9NRI5
• Cytogenetics: 1q42.2
• Gene Summary: This gene encodes a protein with multiple coiled coil motifs which is located in the nucleus, cytoplasm and mitochondria. The protein is involved in neurite outgrowth and cortical development through its interaction with other proteins. This gene is disrupted in a t(1;11)(q42.1;q14.3) translocation which segregates with schizophrenia and related psychiatric disorders in a large Scottish family. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]