MIER1 (NM_001146110) Human Tagged ORF Clone

CAT#: RG228431

MIER1 (tGFP-tagged) - Human mesoderm induction early response 1 homolog (Xenopus laevis) (MIER1), transcript variant 10

Product Images

Circular map for RG228431

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MIER1
• Synonyms: ER1; MI-ER1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG228431 representing NM_001146110
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTATGTTTAATTGGTTTACAGACTGTCTGTGGACTCTTTTCCTGTCAAATTACCAGCCATCTGTTG
  AATCTTCAAGTCCAGGAGGTTCAGCAACATCAGATGACCATGAATTTGATCCATCAGCTGACATGCTGGT
  TCATGATTTTGATGATGAACGAACATTAGAAGAGGAAGAAATGATGGAAGGAGAAACAAACTTCAGCTCT
  GAAATAGAAGATCTTGCAAGGGAAGGCGACATGCCAATTCATGAACTTCTCAGCCTTTATGGTTATGGTA
  GTACTGTTCGACTACCTGAAGAAGATGAGGAAGAGGAAGAAGAGGAAGAAGAAGGTGAAGATGATGAAGA
  TGCTGATAATGATGACAACAGTGGCTGTAGTGGGGAAAATAAAGAGGAGAATATAAAGGATTCATCAGGT
  CAGGAGGATGAAACTCAGTCTTCCAATGATGATCCATCACAATCTGTTGCTTCTCAAGATGCCCAGGAAA
  TAATCCGCCCACGTCGATGTAAATATTTTGATACAAATAGTGAAGTAGAAGAAGAATCTGAAGAAGATGA
  AGATTATATTCCATCAGAAGACTGGAAAAAGGAGATTATGGTGGGCTCCATGTTTCAAGCAGAAATTCCA
  GTTGGCATTTGTAGATACAAAGAAAATGAAAAAGTATATGAAAATGATGATCAGCTCCTGTGGGACCCTG
  AGTACTTACCAGAAGATAAAGTGATTATATTTCTTAAAGATGCATCTAGAAGAACAGGTGATGAGAAGGG
  TGTAGAAGCAATTCCTGAAGGATCTCACATAAAAGACAATGAACAGGCTTTATATGAATTGGTTAAATGC
  AATTTTGATACAGAAGAAGCATTGAGAAGATTAAGATTTAATGTAAAAGCAGCTAGAGAGGAATTATCTG
  TTTGGACAGAGGAAGAGTGTAGAAATTTTGAACAAGGGCTGAAGGCCTATGGAAAGGATTTTCATTTGAT
  TCAGGCTAATAAAGTCCGAACAAGGTCAGTTGGTGAATGTGTAGCATTCTATTACATGTGGAAAAAATCT
  GAACGTTATGATTTCTTTGCTCAGCAAACACGATTTGGAAAGAAGAAATATAATCTTCATCCTGGTGTAA
  CGGATTACATGGATCGTCTTCTAGACGAAAGTGAAAGTGCTGCATCTAGTCGAGCACCATCCCCTCCCCC
  AACTGCATCAAACAGTAGTAACAGCCAGTCTGAGAAAGAAGATGGCACTGTAAGCACTGCTAATCAAAAT
  GGAGTGTCATCTAATGGACCAGGTGAAATATTAAACAAAGAGGAAGTAAAAGTTGAAGGGTTACACATTA
  ATGGACCAACAGGTGGAAATAAGAAACCACTTCATGCAGATATGGATACTAATGGTTATGAAACAGATAA
  CCTTACCACTGACCCAAAACTTGCCCATATGACTGCAAGAAATGAAAATGATTTTGATGAAAAAAGTGAG
  AGACCTGCCAAAAGGCGAAGGGTAAACAGCAATGGAAAAGAAAGTCCAGGTTCTTCTGAATTTTTCCAAG
  AAGCAGTCTCACATGGGAAATTTGAAGAACTTGAAAACACAGATGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG228431 representing NM_001146110
  Red=Cloning site Green=Tags(s)
  
  MFMFNWFTDCLWTLFLSNYQPSVESSSPGGSATSDDHEFDPSADMLVHDFDDERTLEEEEMMEGETNFSS
  EIEDLAREGDMPIHELLSLYGYGSTVRLPEEDEEEEEEEEEGEDDEDADNDDNSGCSGENKEENIKDSSG
  QEDETQSSNDDPSQSVASQDAQEIIRPRRCKYFDTNSEVEEESEEDEDYIPSEDWKKEIMVGSMFQAEIP
  VGICRYKENEKVYENDDQLLWDPEYLPEDKVIIFLKDASRRTGDEKGVEAIPEGSHIKDNEQALYELVKC
  NFDTEEALRRLRFNVKAAREELSVWTEEECRNFEQGLKAYGKDFHLIQANKVRTRSVGECVAFYYMWKKS
  ERYDFFAQQTRFGKKKYNLHPGVTDYMDRLLDESESAASSRAPSPPPTASNSSNSQSEKEDGTVSTANQN
  GVSSNGPGEILNKEEVKVEGLHINGPTGGNKKPLHADMDTNGYETDNLTTDPKLAHMTARNENDFDEKSE
  RPAKRRRVNSNGKESPGSSEFFQEAVSHGKFEELENTDD
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001146110
• ORF Size: 1587 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001146110.2
• RefSeq Size: 5646 bp
• RefSeq ORF: 1590 bp
• Locus ID: 57708
• UniProt ID: Q8N108
• Cytogenetics: 1p31.3
• Gene Summary: This gene encodes a protein that was first identified in Xenopus laevis by its role in a mesoderm induction early response (MIER). The encoded protein functions as a transcriptional regulator. Alternatively spliced transcript variants encode multiple isoforms, some of which lack a C-terminal nuclear localization signal. [provided by RefSeq, May 2013]